Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
Top Cited Papers
- 25 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 18 (3), 253-257
- https://doi.org/10.1038/s41592-021-01061-2
Abstract
Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.Funding Information
- DFG and EFRE
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