Sensitivity-Enhanced Optical Fiber Biosensor Based on Coupling Effect Between SPR and LSPR
- 20 August 2018
- journal article
- research article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Sensors Journal
- Vol. 18 (20), 8303-8310
- https://doi.org/10.1109/jsen.2018.2866169
Abstract
An optical fiber biosensor based on gold nanoparticles and protein A co-modified Au film coated photonic crystal fiber (Au-PCF) is proposed and demonstrated for human IgG detection. For the electric field coupling effect between surface plasmon resonance (SPR) of Au film and localized surface plasmon resonance (LSPR) of gold nanoparticles, the refractive index sensitivity can be enhanced significantly. Besides, goat anti-human IgG is immobilized by protein A modified on the gold nanoparticles surface to interact with human IgG. Protein A can specifically bind the Fc region of the antibodies and has a high degree of orientation for capturing antibodies. Experimental results indicate that the refractive index sensitivity of Au-PCF sensor modified gold nanoparticles reaches 3915 nm/RIU, which is about 1.6 times higher than the Au-PCF sensor without gold nanoparticles modified. The lowest detection limit of human IgG based on gold nanoparticles and protein A co-modified Au-PCF sensor is 37 ng/mL, which is about 2.4 times and 6.3 times lower than gold nanoparticles-Au-PCF sensor and Au-PCF sensor, respectively. Such a sensitivity enhanced optical fiber biosensor based on SPR-LSPR coupling effect has potential application in clinical disease diagnosis and immunoassays.Keywords
Funding Information
- Natural Science Foundation of Liaoning Province (201602262)
- Fundamental Research Funds for the Central Universities (N160405001)
This publication has 34 references indexed in Scilit:
- Enhanced wavelength modulation SPR biosensor based on gold nanorods for immunoglobulin detectionTalanta, 2013
- A protein A modified Au–graphene oxide composite as an enhanced sensing platform for SPR-based immunoassayThe Analyst, 2013
- Wavelength-based localized surface plasmon resonance optical fiber biosensorSensors and Actuators B: Chemical, 2013
- Probing the Ultimate Limits of Plasmonic EnhancementScience, 2012
- Single Unlabeled Protein Detection on Individual Plasmonic NanoparticlesNano Letters, 2012
- Effects of surface density and size of gold nanoparticles in a fiber-optic localized surface plasmon resonance sensor and its application to peptide detectionMeasurement Science and Technology, 2010
- Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensorBiosensors and Bioelectronics, 2009
- Directed self-assembly of gold binding polypeptide-protein A fusion proteins for development of gold nanoparticle-based SPR immunosensorsBiosensors and Bioelectronics, 2009
- Structure of Dithiothreitol Monolayers on Au(111)The Journal of Physical Chemistry B, 2003
- Surface plasmon resonance hydrogen sensor using an optical fibre*Measurement Science and Technology, 2001