Chemical Analysis and Cytotoxic Evaluation of Asphodelus aestivus Brot. Flowers
- 4 May 2021
- journal article
- research article
- Published by Egypts Presidential Specialized Council for Education and Scientific Research in Egyptian Journal of Chemistry
- Vol. 64 (9), 5167-5174
- https://doi.org/10.21608/ejchem.2021.70226.3546
Abstract
Asphodelus are among many plant species used in traditional medicine, they are used by native peoples for various pathologies. Asphodelus aestivus Brot. is one of Asphodelus species which is considered an important wild medicinal plant growing mainly at Mediterranean region. The present study aims to investigate the chemical constituents and cytotoxic activity of A. aestivus flower. Petroleum ether extract was analyzed by GC/MS assay which revealed the characterization of twenty two compounds, representing 97.14% of total extract. Also, the chromatographic investigation of the aqueous methanol extract led to the isolation of ten phenolics, from which, five compounds have been isolated for the first time from A. aestivus. They were identified as kaempferol (1), kaempferol 3-O-glucoside (2), kaempferol 3,7-di-O-glucoside (3), luteolin 7-O-glucoside )4), isoorientin (5), isoorientin 7-O-glucoside (lutonarin) (6), caffeic acid (7) emodin (8), apigenin 7-O-glucoside (9) and isovitexin-7-O-glucoside (saponarin) (10). Furthermore, LC-ESI-MS chemical profiling managed to detect the minor contents and led to the annotation of thirteen additional metabolites. In addition, the petroleum ether and aqueous methanol extracts were investigated against four human carcinoma cell lines; HepG2, HCT116, MCF7 and A549 at concentration of 100 mg/mL. The results showed moderate % of inhibition against MCF7 (63.8 and 68.4) followed by HEPG2 (52.3 and 65.7), respectively. Both extracts showed weak inhibition (5.7-14.3%) on HCT-116 and A549. To the best of our knowledge, this is considered the first report of the chemical analysis and biological evaluation of A. aestivus flowers.Keywords
This publication has 18 references indexed in Scilit:
- LC‐MS‐based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majoranaPhytochemical Analysis, 2015
- Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacumtissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation methodChemistry Central Journal, 2014
- Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MSMolecules, 2014
- Two new flavonol glycosides and biological activities ofDiplotaxis harra(Forssk.) Boiss.Natural Product Research, 2013
- Flavonoid constituents and biological screening ofAstragalus bombycinusBoiss.Natural Product Research, 2013
- A study of oxidation products of free polyunsaturated fatty acids in mechanically deboned meatJournal of Food Composition and Analysis, 2009
- Fed-batch Production of Gluconic Acid by Terpene-treated Aspergillus niger SporesApplied Biochemistry and Biotechnology, 2008
- Medicinal plants popularly used in the villages of Yunt Mountain(Manisa-Turkey)Fitoterapia, 2008
- Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometryJournal of the American Society for Mass Spectrometry, 2007
- Electrospray mass spectrometry of phospholipidsMass Spectrometry Reviews, 2003