Phenol glycosides extract of Fructus Ligustri Lucidi attenuated depressive‐like behaviors by suppressing neuroinflammation in hypothalamus of mice
- 30 June 2020
- journal article
- research article
- Published by Wiley in Phytotherapy Research
- Vol. 34 (12), 3273-3286
- https://doi.org/10.1002/ptr.6777
Abstract
Depression is partially caused by inflammation in central nervous system. This study investigated the ameliorative effects of phenol glycosides (PG) from Ligustrum lucidum Ait. (Oleaceae) on neuroinflammation and depressive‐like behavior in mice hypothalamus as well as the molecular mechanism. Mice were administered with PG extract for 2 weeks prior to treatment with LPS. The mice treated with PG extract showed resistance to LPS‐induced reduction in body weight and LPS‐induced depressive‐like behaviors shown by sucrose preference, tail suspension test, forced swimming test and open field test. LPS‐induced activation of microglial cells and elevation in protein expression of inflammatory cytokines including IL‐1β, RANTES and MCP‐1 in hypothalamus of mice were abrogated by pre‐treatment with PG extract. This extract down‐regulated expression of TLR4, MyD88, NLRP3, renin and angiotensin II and decreased proportional area of Iba‐1+ microglias in hypothalamus. Pre‐treatment with PG extract inhibited LPS‐triggered activation of CaSR/Gα11 signaling, stimulated 1‐OHase expression in hypothalamus, and enhanced circulating 1,25(OH)2D3 level. Overall, pre‐treatment with PG extract ameliorated LPS‐induced depressive‐like behaviors by repressing neuroinflammation in mice hypothalamus which was attributed to its suppression on activation of microglia and production of inflammatory cytokines via acting on TLR4 pathway, CaSR and RAS cascade associated with improving vitamin D metabolism.Keywords
Funding Information
- Ministry of Education of the People's Republic of China (IRT1270)
- Program of Shanghai Academic Research Leader (19XD1423800)
- Sanming Project of Medicine in Shenzhen (SZSM201808072)
- Program for Innovative Research Team of Ningbo Novel Photoelectric Materials and Devices
This publication has 52 references indexed in Scilit:
- Blockade of brain angiotensin II AT1 receptors ameliorates stress, anxiety, brain inflammation and ischemia: Therapeutic implicationsPsychoneuroendocrinology, 2011
- Vitamin D Receptor Attenuates Renal Fibrosis by Suppressing the Renin-Angiotensin SystemJournal of the American Society of Nephrology, 2010
- Depression and bone massAnnals of the New York Academy of Sciences, 2010
- New Secoiridoid Glucosides fromLigustrum lucidumInduce ERK and CREB Phosphorylation in Cultured Cortical NeuronsPlanta Medica, 2010
- Long-term therapeutic effect of vitamin D analog doxercalciferol on diabetic nephropathy: strong synergism with AT1 receptor antagonistAmerican Journal of Physiology-Renal Physiology, 2009
- Fructus ligustri lucidi extract improves calcium balance and modulates the calciotropic hormone level and vitamin D-dependent gene expression in aged ovariectomized ratsMenopause, 2008
- Improvement of bone properties and enhancement of mineralization by ethanol extract of Fructus Ligustri LucidiBritish Journal of Nutrition, 2008
- From inflammation to sickness and depression: when the immune system subjugates the brainNature Reviews Neuroscience, 2008
- Fructus Ligustrum lucidi inhibits inflammatory mediator release through inhibition of nuclear factor-κB in mouse peritoneal macrophagesJournal of Pharmacy and Pharmacology, 2007
- Calcium receptor stimulates chemotaxis and secretion of MCP-1 in GnRH neurons in vitro: potential impact on reduced GnRH neuron population in CaR-null miceAmerican Journal of Physiology-Endocrinology and Metabolism, 2007