Box–Behnken design optimisation of a green novel nanobio-based reagent for rapid visualisation of latent fingerprints on wet, non-porous substrates
- 3 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 43 (4), 881-898
- https://doi.org/10.1007/s10529-020-03052-3
Abstract
Objective Optimisation of the green novel nanobio-based reagent (NBR) for rapid visualisation of groomed fingerprints on wet non-porous substrates using response surface methodology and assessment of its stability and sensitivity were attempted for forensic applications. Results Scanning electron microscopy images demonstrated successful attachments of NBR onto the constituents of fingerprints on the substrates. The highest average quality of visualised fingerprints was attained at the optimum condition (100 mg of CRL; 75 mg of acid-functionalised multi-walled carbon nanotubes; 5 h of immobilisation). The NBR produced comparable average quality of fingerprints with the commercially available small particle reagent, even after 4 weeks of storage (without any preservatives) in both chilled and sultry conditions. The NBR was sensitive enough to visualise the increasingly weaker fingerprints, particularly on glass slides. Conclusion The optimised novel NBR could be the relatively greener option for visualising latent fingerprints on wet, non-porous substrates for forensic applications.Keywords
Funding Information
- Universiti Teknologi Malaysia (Q.J130000.2526.12H51)
- Ministry of Higher Education (R.J130000.7854.4F990)
This publication has 47 references indexed in Scilit:
- Small particle reagent based on crystal violet dye for developing latent fingerprints on non-porous wet surfacesEgyptian Journal of Forensic Sciences, 2015
- Immobilization of lipases on hydrophobic supports involves the open form of the enzymeEnzyme and Microbial Technology, 2015
- Adsorption of lipase from Candida rugosa on multi walled carbon nanotubesJournal of Industrial and Engineering Chemistry, 2013
- Composition of fingermark residue: A qualitative and quantitative reviewForensic Science International, 2012
- Enzymatic esterification of fatty acid esters by tetraethylammonium amino acid ionic liquids-coated Candida rugosa lipaseJournal of Molecular Catalysis B: Enzymatic, 2012
- Active biocatalysts based on Candida rugosa lipase immobilized in vesicular silicaProcess Biochemistry, 2012
- An assessment of the effectiveness of 5-methylthioninhydrin within dual action reagents for latent fingerprint development on paper substratesScience & Justice, 2012
- Response surface methodology (RSM) as a tool for optimization in analytical chemistryTalanta, 2008
- Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoterJournal of Industrial Microbiology & Biotechnology, 2007
- Morphological, biochemical and kinetic properties of lipase fromCandida rugosaimmobilized in zirconium phosphateBiocatalysis and Biotransformation, 2007