Integrative analysis of transcriptomic data for identification of T-cell activation-related mRNA signatures indicative of preterm birth
Open Access
- 27 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-12
- https://doi.org/10.1038/s41598-021-81834-z
Abstract
Preterm birth (PTB), defined as birth at less than 37 weeks of gestation, is a major determinant of neonatal mortality and morbidity. Early diagnosis of PTB risk followed by protective interventions are essential to reduce adverse neonatal outcomes. However, due to the redundant nature of the clinical conditions with other diseases, PTB-associated clinical parameters are poor predictors of PTB. To identify molecular signatures predictive of PTB with high accuracy, we performed mRNA sequencing analysis of PTB patients and full-term birth (FTB) controls in Korean population and identified differentially expressed genes (DEGs) as well as cellular pathways represented by the DEGs between PTB and FTB. By integrating the gene expression profiles of different ethnic groups from previous studies, we identified the core T-cell activation pathway associated with PTB, which was shared among all previous datasets, and selected three representative DEGs (CYLD, TFRC, and RIPK2) from the core pathway as mRNA signatures predictive of PTB. We confirmed the dysregulation of the candidate predictors and the core T-cell activation pathway in an independent cohort. Our results suggest that CYLD, TFRC, and RIPK2 are potentially reliable predictors for PTB.Keywords
Funding Information
- Korean National Cancer Center (1810861-3)
- Korea Health Industry Development Institute (HI18C0378)
- National Research Foundation of Korea (NRF-2020R1A2C3011850)
This publication has 49 references indexed in Scilit:
- Changes in the perinatal outcomes of twin pregnancies delivered at a tertiary referral center in Korea during a 24-year period from 1995 to 2018Obstetrics & Gynecology Science, 2020
- Preterm Birth: A Narrative Review of the Current Evidence on Nutritional and Bioactive Solutions for Risk ReductionNutrients, 2019
- Initiation of human parturition: signaling from senescent fetal tissues via extracellular vesicle mediated paracrine mechanismObstetrics & Gynecology Science, 2019
- Risk Factors for Preterm Birth in Advanced Maternal AgeJOURNAL OF THE KOREAN SOCIETY OF MATERNAL AND CHILD HEALTH, 2018
- Differential expression and methylation of integrin subunit alpha 11 and thrombospondin in the amnion of preterm birthObstetrics & Gynecology Science, 2018
- Long-Term Impact of Preterm BirthClinics in Perinatology, 2017
- Preterm Birth and Its Long-Term Effects: Methylation to MechanismsBiology, 2014
- Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000The Lancet, 2012
- Limits of Human Viability in the United States: A Medicolegal ReviewPEDIATRICS, 2011
- Cognitive and Behavioral Outcomes of School-Aged Children Who Were Born PretermJama-Journal Of The American Medical Association, 2002