LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis
- 13 January 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in Cell Cycle
- Vol. 19 (3), 300-316
- https://doi.org/10.1080/15384101.2019.1708016
Abstract
Background: Long non-coding RNAs (lncRNAs) have been reported to participate in many diseases including non-small cell lung cancer (NSCLC), thus our objective was to investigate the impact of lncRNA SBF2-AS1 modulating microRNA-302a (miR-302a) expression on radiosensitivity of NSCLC. Methods: The expression of SBF2-AS1, miR-302a and muscleblind-like 3 (MBNL3) in NSCLC tissues of the radiotherapy-sensitive and radiotherapy-resistant groups was tested. The radiosensitivity of parent and resistant strains (NCI-H1299 and NCI-H1299R cells) was detected. Further, cells were treated with si-SBF2-AS1 and miR-302a mimics to determine their roles in proliferation and apoptosis of parent strain and resistant strain cells as well as transfected cells. The in-vivo growth capacity of the cells and the effect of radiotherapy on tumor size of NSCLC were detected. Results: Up-regulated SBF2-AS1 and MBNL3 and down-regulated miR-302a in NSCLC tissues of the radiotherapy resistant group. Overexpression of SBF2-AS1 and MBNL3 and low expression of miR-302a were witnessed in NCI-H1299R cells. Down-regulated SBF2-AS1 or up-regulated miR-302a suppressed the proliferation while boosted the apoptosis of NCI-H1299 cells and decreased the radioresistance of the NCI-H1299R cells. Silencing SBF2-AS1 or up-regulating miR-302a restrained tumor growth in vivo. Conclusion: Our study presents that high expression of miR-302a or inhibition of SBF2-AS1 can enhance the radiosensitivity and apoptosis of NSCLC cells through downregulation of MBNL3, which is a therapeutic target for NSCLC.Keywords
Funding Information
- General Program of Natural Science Foundation of Liaoning Province (2015020464)
- Major Science and Technology Platform of Liaoning Higher Institution (2916009)
- Science Research Foundation for Absorbed Talents Program of Liaoning Cancer Hospital and Special Project of Applied Technology of Population and Health by Shenyang Science and Technology Plan (18-014-4-04)
This publication has 30 references indexed in Scilit:
- Long noncoding RNA SBF2-AS1 act as a ceRNA to modulate cell proliferation via binding with miR-188-5p in acute myeloid leukemiaArtificial Cells, Nanomedicine, and Biotechnology, 2019
- MicroRNA‐331‐3p inhibits epithelial‐mesenchymal transition by targeting ErbB2 and VAV2 through the Rac1/PAK1/β‐catenin axis in non‐small‐cell lung cancerCancer Science, 2019
- Research on circadian clock genes in non-small-cell lung carcinomaChronobiology International, 2019
- Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Endometrial Cancer Cell Proliferation and Migration through Delivery of Exogenous miR-302aStem Cells International, 2019
- LncRNA SNHG16 promotes cell proliferation through miR-302a-3p/FGF19 axis in hepatocellular carcinomaNeoplasma, 2019
- miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer developmentJournal of Experimental & Clinical Cancer Research, 2018
- MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRASCellular Physiology and Biochemistry, 2017
- Prognostic Implications of miR-302a/b/c/d in Human Gastric CancerPathology and Oncology Research, 2017
- MicroRNA miR-302 Inhibits the Tumorigenecity of Human Pluripotent Stem Cells by Coordinate Suppression of the CDK2 and CDK4/6 Cell Cycle PathwaysCancer Research, 2010
- Genome-Wide Survey and Expression Profiling of CCCH-Zinc Finger Family Reveals a Functional Module in Macrophage ActivationPLOS ONE, 2008