Notch-Regulated Dendritic Cells Restrain Inflammation-Associated Colorectal Carcinogenesis
- 13 January 2021
- journal article
- research article
- Published by American Association for Cancer Research (AACR) in Cancer Immunology Research
- Vol. 9 (3), 348-361
- https://doi.org/10.1158/2326-6066.cir-20-0428
Abstract
Conventional dendritic cells (cDCs) play a central role in T-cell anti-tumor responses. We studied the significance of Notch-regulated DC immune responses in a mouse model of colitis-associated colorectal cancer (CRC) in which there is epithelial down-regulation of Notch/Hes1 signaling. This defect phenocopies that caused by GMDS (GDP-mannose 4,6-dehydratase) mutation in human CRCs. We found that, although wild-type immune cells restrained dysplasia progression and decreased incidence of adenocarcinoma in chimeric mice, the immune system with Notch2 deleted in all blood lineages or in only DCs promoted inflammation-associated transformation. Notch2 signaling deficiency not only impaired cDC terminal differentiation, but also down-regulated CCR7 expression, reduced DC migration and suppressed antigen cross-presentation to CD8+ T cells. Transfer of Notch-primed DCs restrained inflammation-associated dysplasia progression. Consistent with the mouse data, we observed a correlation between infiltrating cDC1 and Notch2 signaling in human CRCs and found that GMDS mutant CRCs showed decreased CCR7 expression and suppressed cDC1 signature gene expression. Suppressed cDC1 gene signature expression in human CRC was associated with a poor prognosis. In summary, our study supports an important role for Notch2 signaling in cDC1-mediated anti-tumor immunity and indicates that Notch2-controlled DCs restrain inflammation-associated colon cancer development in mice.Keywords
Funding Information
- NCI (CA222064)
- NIH (HL103827)
This publication has 49 references indexed in Scilit:
- The Cancer Genome Atlas Pan-Cancer analysis projectNature Genetics, 2013
- Human Tissues Contain CD141hi Cross-Presenting Dendritic Cells with Functional Homology to Mouse CD103+ Nonlymphoid Dendritic CellsImmunity, 2012
- Deciphering the transcriptional network of the dendritic cell lineageNature Immunology, 2012
- Notch2 Receptor Signaling Controls Functional Differentiation of Dendritic Cells in the Spleen and IntestineImmunity, 2011
- Immunity, Inflammation, and CancerCell, 2010
- Batf3 Deficiency Reveals a Critical Role for CD8α + Dendritic Cells in Cytotoxic T Cell ImmunityScience, 2008
- Notch-dependent control of myelopoiesis is regulated by fucosylationBlood, 2008
- DNA damage induced by chronic inflammation contributes to colon carcinogenesis in miceJCI Insight, 2008
- Notch–RBP-J signaling controls the homeostasis of CD8− dendritic cells in the spleenThe Journal of Experimental Medicine, 2007
- Regulation of dendritic-cell differentiation by bone marrow stroma via different Notch ligandsBlood, 2006