Arabidopsis REM16 acts as a B3 domain transcription factor to promote flowering time via directly binding to the promoters of SOC1 and FT
- 1 August 2020
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 103 (4), 1386-1398
- https://doi.org/10.1111/tpj.14807
Abstract
Actin depolymerizing factor (ADF) is a key modulator for dynamic organization of actin cytoskeleton. Interestingly, it was found that the ADF1 gene silencing delays flowering, but its mechanism remains unclear. In this study, ADF1 was used as a bait to screen its interacting proteins by the yeast two-hybrid (Y2H) system. One of them, the REM16 transcription factor was identified. As one of the AP2/B3-like transcriptional factor family members, the REM16 contains two B3 domains and its transcript levels kept increasing during the floral transition stage. Overexpression of REM16 accelerates flowering while silencing of REM16 delays flowering. Gene expression analysis indicated that the key flowering activation genes such as CONSTANS (CO), FLOWERING LOCUS T (FT), LEAFY (LFY) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS (SOC1) were upregulated in the REM16 overexpression lines, while the transcription of the flowering suppression gene FLOWERING LOCUS C (FLC) was decreased. In contrast, the REM16 gene silencing lines contained lower transcript levels of the CO, FT, LFY and SOC1 but higher transcript levels of the FLC compared with the wild-type plants. It was proved that REM16 could directly bind to the promoter regions of SOC1 and FT by in vitro and in vivo assays. Genetic analysis supported that REM16 acts upstream of SOC1 and FT in flowering pathways. All these studies provided strong evidence demonstrating that REM16 promotes flowering by directly activating SOC1 and FT.Keywords
Funding Information
- National Natural Science Foundation of China (31870255, 31571389, 31600224)
This publication has 70 references indexed in Scilit:
- The rpg4-Mediated Resistance to Wheat Stem Rust (Puccinia graminis) in Barley (Hordeum vulgare) Requires Rpg5, a Second NBS-LRR Gene, and an Actin Depolymerization FactorMolecular Plant-Microbe Interactions®, 2013
- ThemicroRNA156-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3Module Regulates Ambient Temperature-Responsive Flowering viaFLOWERING LOCUS Tin ArabidopsisPlant Physiology, 2012
- Arabidopsis Actin Depolymerizing Factor4 Modulates the Stochastic Dynamic Behavior of Actin Filaments in the Cortical Array of Epidermal CellsPlant Cell, 2011
- VERDANDIIs a Direct Target of the MADS Domain Ovule Identity Complex and Affects Embryo Sac Differentiation inArabidopsisPlant Cell, 2010
- Nuclear Functions of ActinCold Spring Harbor Perspectives in Biology, 2010
- Actin-Depolymerizing Factor2-Mediated Actin Dynamics Are Essential for Root-Knot Nematode Infection ofArabidopsisPlant Cell, 2009
- Arabidopsis Actin-Depolymerizing Factor AtADF4 Mediates Defense Signal Transduction Triggered by thePseudomonas syringaeEffector AvrPphBPlant Physiology, 2009
- ACTIN DEPOLYMERIZING FACTOR9 controls development and gene expression in ArabidopsisPlant Molecular Biology, 2008
- TheGIGANTEA-Regulated MicroRNA172 Mediates Photoperiodic Flowering Independent ofCONSTANSinArabidopsisPlant Cell, 2007
- Repression of theLEAFY COTYLEDON 1/B3Regulatory Network in Plant Embryo Development byVP1/ABSCISIC ACID INSENSITIVE 3-LIKEB3 GenesPlant Physiology, 2006