Oligo(l-glutamic acids) in Calcium Phosphate Precipitation: Chain Length Effect
- 29 June 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in The Journal of Physical Chemistry B
- Vol. 124 (29), 6278-6287
- https://doi.org/10.1021/acs.jpcb.0c01689
Abstract
The understanding of calcium phosphate precipitation is of major interest in different fields of science, including medicine, biomaterials, and physical chemistry. The presence of additive biomacromolecules has been known to influence various stages of the precipitation process from nucleation to crystal growth. In the current work, well-defined sequences of short, negatively charged peptides, oligo(l-glutamic acids), were utilized as a model, inspired by contiguous sequences of acidic amino acids in natural biomineralization proteins. The precipitate morphology and phases, the element time profile in solution and in the precipitates, as well as the kinetics during the precipitation process were analyzed to explain the effect of these short peptides on calcium phosphate precipitation. The results show that peptides can delay the phase transformation of an amorphous precursor phase to hydroxyapatite and that there is an optimal chain length for this effect at a given concentration of peptide. This study is the first part of a two-part series and is followed by a subsequent work to reveal the mechanism by which these short peptides influence the calcium phosphate precipitation.Keywords
Funding Information
- Division of Earth Sciences (1652237)
- Division of Electrical, Communications and Cyber Systems (1542100)
- University of Akron
This publication has 37 references indexed in Scilit:
- Calcium phosphates in biomedical applications: materials for the future?Materials Today, 2015
- Biomineralization: A confluence of materials science, biophysics, proteomics, and evolutionary biologyMRS Bulletin, 2015
- The Mineral–Collagen Interface in BoneCalcified Tissue International, 2015
- Crystals competing for spaceNature Materials, 2014
- Biomimetic Calcium Phosphate Crystal Mineralization on Electrospun Cellulose-Based ScaffoldsACS Applied Materials & Interfaces, 2011
- Characterization of a calcium phosphate–TiO2 nanotube composite layer for biomedical applicationsMaterials Science and Engineering: C, 2011
- Coating Electrospun Poly(ε-caprolactone) Fibers with Gelatin and Calcium Phosphate and Their Use as Biomimetic Scaffolds for Bone Tissue EngineeringLangmuir, 2008
- Phosphorylated Proteins and Control over Apatite Nucleation, Crystal Growth, and InhibitionChemical Reviews, 2008
- Biomimetic calcium phosphate coating on electrospun poly(ɛ-caprolactone) scaffolds for bone tissue engineeringChemical Engineering Journal, 2008
- Local induction of calcium phosphate formation on TiO2 coatings on titanium via surface treatment with a CO2 laserJournal of Biomedical Materials Research, 2003