Development of Ogura CMS restorers in Brassica oleracea subspecies via direct RfoB gene transformation
- 6 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 134 (4), 1123-1132
- https://doi.org/10.1007/s00122-020-03757-z
Abstract
Key message The Ogura CMS RfoB restorer developing via RfoB gene transformation was utilized to produce specific morphological Ogura CMS restorers and clubroot resistance lines in Brassica oleracea subspecies. Abstract Brassica oleracea vegetables including cabbage, cauliflower, kohlrabi, Brussels sprouts and Chinese kale are morphologically very different despite being members of the same species. The Ogura cytoplasmic male sterility (CMS) system is the most stable strategy for the hybrid breeding of these species. However, this limits the utilization of some excellent genes due to the lack of fertile restorer genes in the system. Herein, to efficaciously use Ogura CMS, the Ogura CMS RfoB restorer was produced by transforming the modified RfoB restorer gene into the Ogura CMS line ‘CMS2016’ of B. oleracea var. capitata. This gene was shown to recover fertility of natural Ogura CMS lines in B. oleracea subspecies and create transient Ogura CMS RfoB restorers such as the clubroot resistance Ogura CMS RfoB restorer. Interestingly, clubroot resistant individuals without transgenic elements were screened in the progenies of hybrids between B. oleracea inbred lines and the clubroot resistance Ogura CMS RfoB restorer. In addition, 18 different morphological Ogura CMS restorers were developed to specifically recover fertile of Ogura CMS cultivars in B. oleracea subspecies.Keywords
Funding Information
- National Key Research and Development Program of China (2017YFD0101804)
- Technology Innovation and Application Development Program of Chongqing (cstc2019.jscx-gksbX0117)
- Fundamental Research Funds for the Central Universities (XDJK2019B046, SWU118063)
- Fundamental Research Funds for the Central Universities (XDJK2018C090)
This publication has 34 references indexed in Scilit:
- Superior CMS (Ogura) lines with better combining ability improve yield and maturity in cauliflower (Brassica oleracea var. botrytis)Euphytica, 2011
- Physical localization and genetic mapping of the fertility restoration geneRfoin canola (Brassica napusL.)Genome, 2009
- Agrobacterium-mediated transformation of Brassica napus and Brassica oleraceaNature Protocols, 2008
- Cytoplasmic male sterility: a window to the world of plant mitochondrial–nuclear interactionsTrends in Genetics, 2007
- A novel locus for clubroot resistance in Brassica rapa and its linkage markersTheoretical and Applied Genetics, 2003
- The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeatsThe Plant Journal, 2003
- Protoplast fusion-derived Ogura male sterile cauliflower with cold tolerancePlant Cell Reports, 1992
- Transient Gene Expression in Protoplasts of Phaseolus vulgaris Isolated from a Cell Suspension CulturePlant Physiology, 1991
- Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusionTheoretical and Applied Genetics, 1990
- Cytoplasmic male sterility in rapeseed (Brassica napus L.): female fertility of restored rapeseed with "Ogura" and cybrids cytoplasmsGenome, 1988