The low survival rate in Sumatran fish fingerlings transportation is a problem related to metabolic disturbances that cause death. The addition of nutmeg seed oil in the transportation medium is expected to reduce the rate of respiration and metabolism, so that fish mortality can be minimized. The purpose of this study was to determine the effective concentration of nutmeg seed oil to maintain the highest survival rate of Sumatran fish fry transported during treatment and to analyze its effect on induction time and recovery time. This research was carried out from January to March 2021 in the Ciparanje wet laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research was conducted experimentally using a factorial randomized group design (FRGD) consisting of two factors, namely the concentration of four levels (0.03, 0.06, 0.09 ml/L and control) and duration of three levels (3, 5, and 7 hours) which was repeated three times. This research uses a closed transportation system. Parameters observed were induction time and conscious recovery time of test fish, post-transportation and post-maintenance survival for 7 days and water quality consisting of temperature, DO, pH, and ammonia. The results showed that the effective nutmeg seed oil for the anesthesia of Sumatran fish fry transported was 0.03 ml/L with a transportation duration of 3 hours because it had an induction time of 08:07, recovery time of 02:41 and postoperative survival rate. transportation by 98.33% and post-maintenance for 7 days by 91.49%. Temperature parameters are 22.7oC, DO is ±10.3 mg/L, pH is ± 6.83 and ammonia is ±0.0010 mg/L.