Non-Invasive Transcranial Nano-Pulsed Laser Therapy Ameliorates Cognitive Function and Prevents Aberrant Migration of Neural Progenitor Cells in the Hippocampus of Rats Subjected to Traumatic Brain Injury
- 15 April 2020
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Neurotrauma
- Vol. 37 (8), 1108-1123
- https://doi.org/10.1089/neu.2019.6534
Abstract
Traumatic brain injury (TBI) can lead to chronic diseases, including neurodegenerative disorders and epilepsy. The hippocampus, one of the most affected brain region after TBI, plays a critical role in learning and memory and is one of the only two regions in the brain in which new neurons are generated throughout life from neural stem cells (NSC) in the dentate gyrus (DG). These cells migrate into the granular layer where they integrate into the hippocampus circuitry. While increased proliferation of NSC in the hippocampus is known to occur shortly after injury, reduced neuronal maturation and aberrant migration of progenitor cells in the hilus contribute to cognitive and neurological dysfunctions, including epilepsy. Here, we tested the ability of a novel, proprietary non-invasive nano-pulsed laser therapy (NPLT), that combines near-infrared laser light (808 nm) and laser-generated, low-energy optoacoustic waves, to mitigate TBI-driven impairments in neurogenesis and cognitive function in the rat fluid percussion injury model. We show that injured rats treated with NPLT performed significantly better in a hippocampus-dependent cognitive test than did sham rats. In the DG, NPLT significantly decreased TBI-dependent impaired maturation and aberrant migration of neural progenitors, while preventing TBI-induced upregulation of specific microRNAs (miRNAs) in NSC. NPLT did not significantly reduce TBI-induced microglia activation in the hippocampus. Our data strongly suggest that NPLT has the potential to be an effective therapeutic tool for the treatment of TBI-induced cognitive dysfunction and dysregulation of neurogenesis, and point to modulation of miRNAs as a possible mechanism mediating its neuroprotective effects.Keywords
This publication has 60 references indexed in Scilit:
- The costs of traumatic brain injury: a literature reviewClinicoEconomics and Outcomes Research, 2013
- Neural stem cells: Brain building blocks and beyondUpsala Journal of Medical Sciences, 2012
- MicroRNAs in Neural Stem Cells and NeurogenesisFrontiers in Neuroscience, 2012
- Improved Cognitive Function After Transcranial, Light-Emitting Diode Treatments in Chronic, Traumatic Brain Injury: Two Case ReportsPhotomedicine and Laser Surgery, 2011
- Temporally Specified Genetic Ablation of Neurogenesis Impairs Cognitive Recovery after Traumatic Brain InjuryJournal of Neuroscience, 2011
- MicroRNA Regulation of Neural Stem Cells and Neurogenesis: Figure 1.Journal of Neuroscience, 2010
- Epigenetic choreographers of neurogenesis in the adult mammalian brainNature Neuroscience, 2010
- Additive effects of physical exercise and environmental enrichment on adult hippocampal neurogenesis in miceFrontiers in Neuroscience, 2009
- The Timing of Differentiation of Adult Hippocampal Neurons Is Crucial for Spatial MemoryPLoS Biology, 2008
- Neurogenesis in the adult hippocampusCell and tissue research, 2007