Benefits of Zebrafish Xenograft Models in Cancer Research
Open Access
- 11 February 2021
- journal article
- review article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
As a promising in vivo tool for cancer research, zebrafish have been widely applied in various tumor studies. The zebrafish xenograft model is a low-cost, high-throughput tool for cancer research that can be established quickly and requires only a small sample size, which makes it favorite among researchers. Zebrafish patient-derived xenograft (zPDX) models provide promising evidence for short-term clinical treatment. In this review, we discuss the characteristics and advantages of zebrafish, such as their transparent and translucent features, the use of vascular fluorescence imaging, the establishment of metastatic and intracranial orthotopic models, individual pharmacokinetics measurements, and tumor microenvironment. Furthermore, we introduce how these characteristics and advantages are applied other in tumor studies. Finally, we discuss the future direction of the use of zebrafish in tumor studies and provide new ideas for the application of it.Keywords
This publication has 205 references indexed in Scilit:
- Orthotopic transplantation of retinoblastoma cells into vitreous cavity of zebrafish for screening of anticancer drugsMolecular Cancer, 2013
- Imaging zebrafish neural circuitry from whole brain to synapseFrontiers in Neural Circuits, 2013
- In vivo genome editing using a high-efficiency TALEN systemNature, 2012
- In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal RhabdomyosarcomaCancer Cell, 2012
- Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequencyLeukemia, 2012
- Neutrophil‐mediated experimental metastasis is enhanced by VEGFR inhibition in a zebrafish xenograft modelThe Journal of Pathology, 2012
- Calpain 2 is required for the invasion of glioblastoma cells in the zebrafish brain microenvironmentJournal of Neuroscience Research, 2011
- Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discoveryBritish Journal of Pharmacology, 2008
- Transparent Adult Zebrafish as a Tool for In Vivo Transplantation AnalysisCell Stem Cell, 2008
- In vivo drug discovery in the zebrafishNature Reviews Drug Discovery, 2005