Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models
Open Access
- 7 December 2016
- journal article
- Published by Portico in BoneKEy Reports
- Vol. 5, 851
- https://doi.org/10.1038/bonekey.2016.84
Abstract
Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.Keywords
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