Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe
- 21 April 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Organic & Biomolecular Chemistry
- Vol. 19 (15), 3389-3395
- https://doi.org/10.1039/d0ob02378g
Abstract
Visualization of sub-cellular organelles allows the determination of various cellular processes and the underlying mechanisms. Herein, we report a fluorescent probe, bearing push-pull substituents emitting at 600 nm and its application in cellular imaging. The probe shows dual imaging of mitochondria and nucleoli and maps mitochondrial viscosity in live cells under various physiological variations and show minimum cytotoxicity. Nucleolar staining is confirmed by RNAase digestion.Funding Information
- Science and Engineering Research Board (CRG/2018/004020, ECR/2016/000913)
- Department of Biotechnology, Ministry of Science and Technology, India (BT/PR15214/BRB/10/1449/2015, BT/RLF/Re-entry/45/2015)
- Agence Nationale de la Recherche (19-CE29-0005-01)
This publication has 54 references indexed in Scilit:
- A Self-Calibrating Bipartite Viscosity Sensor for MitochondriaJournal of the American Chemical Society, 2013
- Molecular Rotors as Fluorescent Viscosity Sensors: Molecular Design, Polarity Sensitivity, Dipole Moments Changes, Screening Solvents, and Deactivation Channel of the Excited StatesEuropean Journal of Organic Chemistry, 2011
- Mitochondrial Dysfunction: Common Final Pathway in Brain Aging and Alzheimer’s Disease—Therapeutic AspectsMolecular Neurobiology, 2010
- Effects of CCCP-induced mitochondrial uncoupling and cyclosporin A on cell volume, cell injury and preconditioning protection of isolated rabbit cardiomyocytesJournal of Molecular and Cellular Cardiology, 2003
- Staurosporine induces apoptosis through both caspase-dependent and caspase-independent mechanismsOncogene, 2001
- Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase CellsThe Journal of cell biology, 2001
- Molecular mechanism of staurosporine-induced apoptosis in osteoblastsPharmacological Research, 2000
- [13] Maximum entropy method of data analysis in time-resolved spectroscopyMethods in Enzymology, 1994
- Influx and efflux kinetics of cationic dye binding to respiring mitochondriaBiophysical Chemistry, 1992
- Effect of viscosity and temperature on torsional relaxation of molecular rotorsThe Journal of Physical Chemistry, 1982