Sanguisorba officinalis L. derived from herbal medicine prevents intestinal inflammation by inducing autophagy in macrophages
Open Access
- 19 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 10 (1), 1-13
- https://doi.org/10.1038/s41598-020-65306-4
Abstract
Disturbed activation of autophagy is implicated in the pathogenesis of inflammatory bowel disease. Accordingly, several autophagy-related genes have been identified as Crohn's disease susceptibility genes. We screened the autophagy activators from a library including 3,922 natural extracts using a high-throughput assay system. The extracts identified as autophagy activators were administered to mice with 2% dextran sodium sulfate (DSS). Among the autophagy inducers, Sanguisorba officinalis L. (SO) suppressed DSS-induced colitis. To identify the mechanism by which SO ameliorates colitis, epithelial cell and innate myeloid cells-specific Atg7-deficient mice (Villin-cre; Atg7(f/f) and LysM-cre; Atg7(f/f) mice, respectively) were analyzed. SO-mediated inhibition of colitis was observed in Villin-cre; Atg7(f/f) mice. However, SO and a mixture of its components including catechin acid, ellagic acid, gallic acid, and ziyuglycoside II (Mix(4)) did not suppressed colitis in LysM-cre; Atg7(f/f) mice. In large intestinal macrophages (M phi) of Atg7(f/f) mice, SO and Mix(4) upregulated the expression of marker genes of anti-inflammatory M phi including Arg1, Cd206, and Relma. However, these alterations were not induced in LysM-cre; Atg7(f/f) mice. These findings indicate that SO and its active components ameliorate DSS-induced colitis by providing intestinal M phi with anti-inflammatory profiles via promotion of Atg7-dependent autophagy.This publication has 64 references indexed in Scilit:
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