miR-30c Impedes Glioblastoma Cell Proliferation and Migration by Targeting SOX9
Open Access
- 5 February 2019
- journal article
- research article
- Published by Computers, Materials and Continua (Tech Science Press) in Oncology Research
- Vol. 27 (2), 165-171
- https://doi.org/10.3727/096504018x15193506006164
Abstract
MiR-30c has been acknowledged as a tumor suppressor in various human cancers, such as ovarian cancer, gastric cancer, and prostate cancer. However, the role of miR-30c in glioblastoma (GBM) needs to be investigated. In our study, we found that the expression of miR-30c was significantly downregulated in GBM tissues and cell lines. We found that overexpression of miR-30c inhibited cellular proliferation of GBM cells in vitro and in vivo. More GBM cells were arrested in the G0 phase after miR-30c overexpression. Moreover, we showed that miR-30c overexpression suppressed the migration and invasion of GBM cells. Mechanistically, we found that SOX9 was a direct target of miR-30c in GBM cells. Overexpression of miR-30c inhibited the mRNA and protein levels of SOX9 in GBM cells. Moreover, there was a negative correlation between the expression of miR-30c and SOX9 in GBM tissues. Finally, we showed that restoration of SOX9 in GBM cells reversed the proliferation, migration, and invasion of GBM cells transfected with miR-30c mimic. Collectively, our results demonstrated that miR-30c suppressed the proliferation, migration, and invasion of GBM cells via targeting SOX9.Keywords
This publication has 35 references indexed in Scilit:
- Neural stem cells, the subventricular zone and radiotherapy: implications for treating glioblastomaJournal of Neuro-Oncology, 2016
- Concurrent therapy to enhance radiotherapeutic outcomes in glioblastoma2016
- Immunotherapy for cancer in the central nervous system: Current and future directionsOncoImmunology, 2015
- SOX9-mediated upregulation of LGR5 is important for glioblastoma tumorigenicityBiochemical and Biophysical Research Communications, 2015
- miR-30c negatively regulates the migration and invasion by targeting the immediate early response protein 2 in SMMC-7721 and HepG2 cells.2015
- Low expression of microRNA-30c promotes invasion by inducing epithelial mesenchymal transition in non-small cell lung cancerMolecular Medicine Reports, 2014
- MiR-145 functions as a tumor-suppressive RNA by targeting Sox9 and adducin 3 in human glioma cellsNeuro-Oncology, 2013
- Canadian Recommendations for the Treatment of Recurrent or Progressive Glioblastoma MultiformeCurrent Oncology, 2011
- Exciting New Advances in Neuro-Oncology: The Avenue to a Cure for Malignant GliomaCA: A Cancer Journal for Clinicians, 2010
- Malignant Gliomas in AdultsThe New England Journal of Medicine, 2008