Thermal adaptation of structural dynamics and regulatory function of adenine riboswitch
- 25 February 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in RNA Biology
- Vol. 18 (11), 2007-2015
- https://doi.org/10.1080/15476286.2021.1886755
Abstract
Ligand binding and temperature play important roles in riboswitch RNAs’ structures and functions. However, most studies focused on studying structural dynamics or gene-regulation function of riboswitches from the aspect of ligand, instead of temperature. Here we combined NMR, ITC, stopped-flow and in vivo assays to investigate the ligand-triggered switch of adenine riboswitch from 10 to 45 ºC. Our results demonstrated that at single-nucleotide resolution, structural regions sensed ligand and temperature diversely. Temperature had opposite effects on ligand-binding and gene-regulation of adenine riboswitch. Compared with higher temperature, the RNA bound with its cognate ligand obviously stronger, while its regulatory capacity was weakened at lower temperature. In addition, application of specific-labeled RNAs to the stopped-flow experiments identified the real-time folding of the specific positions upon ligand addition at different temperatures. The kissing loop and internal loop at the riboswitch responded to ligand and temperature differently. The distinct thermo-dynamics of adenine riboswitch exposed here may contribute to the fields of RNA sensors and drug design.Keywords
Funding Information
- National Natural Science Foundation of China (Grant no. 31872628 and 32071300 to Y. Liu)
- Zhangjiang Laboratory Foundation (Grant no. Y93Z011D01 to ZJ. Liu)
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