Compromised Autophagic Effect of Polystyrene Nanoplastics Mediated by Protein Corona Was Recovered after Lysosomal Degradation of Corona
- 15 September 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 54 (18), 11485-11493
- https://doi.org/10.1021/acs.est.0c04097
Abstract
The adverse biological and ecological consequences of plastic debris have become a serious problem worldwide. Evidences have uncovered the accumulation of nanoplastics (NPs) in organisms. In a complex biological environment, proteins are prone to adsorbed onto the NPs' surface and form a protein corona layer, which mediates the interaction of NPs with cells. Here, we discovered the interaction of polystyrene (PS) NPs with protein fetal bovine serum (FBS) and altered cytotoxic effects. Mechanistically, prefabricated FBS protein corona mediated the relief of autophagic flux blockage, autophagosomes accumulation, and lysosomal damage in RAW264.7 cells caused by PS NPs. Using an individual fluorescent protein bovine serum albumin (BSA) as a corona surrogate, we demonstrated that coronal BSA remains, at least partially, on the surface of PS NPs during the initial stage of internalization and protects cell membrane from PS NPs-induced damage. However, along with the degradation of corona in lysosomes, reappearance of cytotoxicity was observed. Herein, we provided a proof of principle of the manipulation of corona on NPs' toxicity and we expect the result will promote the further safety assessment of NPs.Funding Information
- Ministry of Education of the People's Republic of China (XDJK2019TJ001)
- National Natural Science Foundation of China (21974110, 21976145)
This publication has 46 references indexed in Scilit:
- The plastic brain: neurotoxicity of micro- and nanoplasticsParticle and Fibre Toxicology, 2020
- Toxicity of Microplastics and Nanoplastics in Mammalian SystemsInternational Journal of Environmental Research and Public Health, 2020
- Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in miceScience of The Total Environment, 2018
- Microplastics in air: Are we breathing it in?Current Opinion in Environmental Science & Health, 2018
- Trophic transfer and individual impact of nano-sized polystyrene in a four-species freshwater food chainScientific Reports, 2018
- Brain damage and behavioural disorders in fish induced by plastic nanoparticles delivered through the food chainScientific Reports, 2017
- Potential Health Impact of Environmentally Released Micro- and Nanoplastics in the Human Food Production Chain: Experiences from NanotoxicologyEnvironmental Science & Technology, 2015
- Plastic debris in the open oceanProceedings of the National Academy of Sciences of the United States of America, 2014
- Classify plastic waste as hazardousNature, 2013
- Plastic Accumulation in the North Atlantic Subtropical GyreScience, 2010