Test-retest repeatability of ultrasonographic shear wave elastography in a rat liver fibrosis model: toward a quantitative biomarker for preclinical trials
Open Access
- 1 January 2021
- journal article
- research article
- Published by Korean Society of Ultrasound in Medicine in Ultrasonography
- Vol. 40 (1), 126-135
- https://doi.org/10.14366/usg.19088
Abstract
Purpose: This study evaluated the test-retest repeatability and measurement variability of ultrasonographic shear wave elastography (SWE) for liver stiffness in a rat liver fibrosis model. Methods: In 31 Sprague-Dawley rats divided into three groups (high-dose, low- dose, and control), liver fibrosis was induced by intraperitoneal administration of thioacetamide for 8 weeks. A dedicated radiographer performed SWE to measure liver stiffness in kilopascals in two sessions at a 3-day interval. We calculated correlations between liver stiffness and histopathologic results, measurement variability in each session using coefficients of variation (CoVs) and interquartile/median (IQR/M), and test-retest repeatability between both sessions using the repeatability coefficient. Results: Different levels of liver fibrosis in each group were successfully induced in the animal model. The mean liver stiffness values were 8.88 +/- 1.48 kPa in the control group, 11.62 +/- 1.70 kPa in the low-dose group, and 11.91 +/- 1.73 kPa in the high-dose group. The correlation between collagen areas and liver stiffness values was moderate (r=0.6). In all groups, the second session yielded lower CoVs (i.e., more reliable results) for liver stiffness than the first session, suggesting a training effect for the operator. The mean IQR/M values were also lower in the second session than in the first session, which had four outliers (0.21 vs. 0.12, P<0.001). The test-retest repeatability coefficient was 3.75 kPa and decreased to 2.82 kPa after removing the four outliers. Conclusion: The use of ultrasonographic SWE was confirmed to be feasible and repeatable for evaluating liver fibrosis in preclinical trials. Operator training might reduce variability in liver stiffness measurements.Keywords
Funding Information
- National Research Foundation of Korea (2019R1I1A1A01060744)
This publication has 36 references indexed in Scilit:
- Principles and clinical application of ultrasound elastography for diffuse liver diseaseUltrasonography, 2014
- Quantification of the Pirimicarb Resistance Allele Frequency in Pooled Cotton Aphid (Aphis gossypii Glover) Samples by TaqMan SNP Genotyping AssayPLOS ONE, 2014
- Intraobserver and interobserver variability in computed tomography size and attenuation measurements in patients with renal cell carcinoma receiving antiangiogenic therapy: Implications for alternative response criteriaCancer, 2013
- Quantification of Liver Viscoelasticity with Acoustic Radiation Force: A Study of Hepatic Fibrosis in a Rat ModelJapanese Journal of Clinical Oncology, 2013
- Experimental liver fibrosis research: update on animal models, legal issues and translational aspectsFibrogenesis & Tissue Repair, 2013
- Deletion of clock gene Per2 exacerbates cholestatic liver injury and fibrosis in miceExperimental and Toxicologic Pathology, 2012
- Differential Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Thioacetamide-Induced Chronic Liver InjuryJournal of Korean Medical Science, 2010
- In vivo Quantification of Liver Stiffness in a Rat Model of Hepatic Fibrosis with Acoustic Radiation ForceJapanese Journal of Clinical Oncology, 2009
- Increased stiffness of the rat liver precedes matrix deposition: implications for fibrosisAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2007
- ImageJ for microscopyBioTechniques, 2007