Phytic acid modulates the morphology, immunological response of cytokines and β-defensins in porcine intestine exposed to deoxynivalenol and fumonisin B1
- 14 September 2021
- journal article
- research article
- Published by Brill in World Mycotoxin Journal
- Vol. 14 (3), 441-450
- https://doi.org/10.3920/wmj2020.2648
Abstract
Occurrence of mycotoxins in agricultural products represents a risk for human and animal health. Therefore, there is a requirement of strategies to mitigate their harmful impacts. This study investigated the effects of phytic acid (IP6) on the immunological response of pro-(interleukin (IL)-1β, IL-6, IL-8, IL-10, interferon (IFN)-γ, tumour necrosis factor (TNF)-α) and anti-inflammatory (IL-10) cytokines and β-defensins 1 (pBD-1) and 2 (pBD-2) in porcine jejunal explants exposed to deoxynivalenol (DON) and fumonisin B1 (FB1). The explants were exposed to the following treatments: control, DON (10 μM), DON plus IP6 2.5 mM or 5 mM, FB1 (70 μM), FB1 IP6 plus 2.5 or 5 mM. The expression levels of the cytokines were measured by RT-qPCR. The exposure to FB1 and DON induced intestinal lesions. The presence of 2.5 and 5 mM IP6 inhibited the morphological changes induced by the mycotoxins. The explants exposed to DON showed an increase in the expression of IL-1β and IL-8 and a decrease in the levels of IL-6, IFN-γ, IL-10 and pBD-2. IP6 (5 mM) decreased the expression of IL-8 and increased the expression in pBD-1 and 2 compared to DON alone. FB1 induced a significant decrease in the levels of most of the pro-inflammatory cytokines, IL-10 and pBD-1, and an increase in IL-1β expression. The addition of IP6 5 mM induced significant increase in TNF-α expression compared to FB1. Taken together, the results suggest IP6 modulates immunological changes induced by DON and FB1 on intestinal mucosa resulting in beneficial effects that contribute to intestinal homeostasis and health.This publication has 61 references indexed in Scilit:
- From the Gut to the Brain: Journey and Pathophysiological Effects of the Food-Associated Trichothecene Mycotoxin DeoxynivalenolToxins, 2013
- Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell LineApplied and Environmental Microbiology, 2013
- Antimicrobial Human β-Defensins in the Colon and Their Role in Infectious and Non-Infectious DiseasesPathogens, 2013
- Deoxynivalenol as a New Factor in the Persistence of Intestinal Inflammatory Diseases: An Emerging Hypothesis through Possible Modulation of Th17-Mediated ResponsePLOS ONE, 2013
- Green Tea, Phytic Acid, and Inositol in Combination Reduced the Incidence of Azoxymethane-Induced Colon Tumors in Fisher 344 Male RatsJournal of Medicinal Food, 2011
- The food contaminant fumonisin B1reduces the maturation of porcine CD11R1+intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infectionVeterinary Research, 2009
- Phytic Acid Exposure Alters AflatoxinB1-Induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus)Evidence-Based Complementary and Alternative Medicine, 2009
- The intestine as a possible target for fumonisin toxicityMolecular Nutrition & Food Research, 2007
- The effects of mycotoxins, fungal food contaminants, on the intestinal epithelial cell-derived innate immune responseVeterinary Immunology and Immunopathology, 2005
- Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccinationVeterinary Immunology and Immunopathology, 2004