Tissue‐specific study across the stem of Taxus media identifies a phloem‐specific TmMYB3 involved in the transcriptional regulation of paclitaxel biosynthesis
- 30 January 2020
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 103 (1), 95-110
- https://doi.org/10.1111/tpj.14710
Abstract
Taxus stem barks can be used for paclitaxel extraction. However, the composition of taxoids across the whole stem and stem tissue‐specificity of paclitaxel biosynthesis‐related enzymes remain largely unknown. Cultivated Taxus media trees were used for chemical composition and protein analyses of major stem tissues using an integrated metabolomic and proteomic approach, and the role of TmMYB3 in paclitaxel biosynthesis was investigated. The metabolomic landscape analysis showed differences in stem tissue‐specific accumulation of metabolites. Phytochemical analysis revealed that paclitaxel highly accumulated in the phloem. Ten key enzymes involved in paclitaxel biosynthesis were identified, most of which were predominantly produced in the phloem. The full‐length sequence of TmMYB3 and partial promoter sequences of five paclitaxel biosynthesis‐related genes were isolated. Several MYB recognition elements were found in the promoters of TBT, DBTNBT and TS. Further in vitro and in vivo investigations indicated that TmMYB3 was involved in paclitaxel biosynthesis by activating the expression of TBT and TS. Differences in the taxoid composition of different stem tissues suggested that the whole stem of T. media has potential for biotechnological applications. Phloem‐specific TmMYB3 plays a role in the transcriptional regulation of paclitaxel biosynthesis, and may explain the phloem‐specific accumulation of paclitaxel.Keywords
This publication has 63 references indexed in Scilit:
- Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all diseaseJournal of Experimental Botany, 2013
- Bark removal and population structure of Taxus wallichiana Zucc. in a temperate mixed conifer forest of western HimalayaEnvironmental Monitoring and Assessment, 2012
- Transcriptional profile of Taxus chinensis cells in response to methyl jasmonateBMC Genomics, 2012
- Functional analysis of a WRKY transcription factor involved in transcriptional activation of the DBAT gene in Taxus chinensisPlant Biology, 2012
- Rapid Qualitative and Quantitative Determination of Seven Valuable Taxanes from VariousTaxusSpecies by UFLC‐ESI‐MS and UFLC‐DADPlanta Medica, 2010
- Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactionsNature Protocols, 2007
- Cytochrome P450 oxygenases of Taxol biosynthesisPhytochemistry Reviews, 2006
- How Far Can a Molecule of Weak Acid Travel in the Apoplast or Xylem?Plant Physiology, 2006
- Taxane Diterpenoids from the Stem Bark of Taxus maireiJournal of Natural Products, 2001