Multiomics-based dissection of citrus flavonoid metabolism using a Citrus reticulata × Poncirus trifoliata population
Open Access
- 1 March 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Horticulture Research
- Vol. 8 (1), 1-12
- https://doi.org/10.1038/s41438-021-00472-8
Abstract
Deciphering the genetic basis of plant secondary metabolism will provide useful insights for genetic improvement and enhance our fundamental understanding of plant biological processes. Although citrus plants are among the most important fruit crops worldwide, the genetic basis of secondary metabolism in these plants is largely unknown. Here, we use a high-density linkage map to dissect large-scale flavonoid metabolic traits measured in different tissues (young leaf, old leaf, mature pericarp, and mature pulp) of an F1 pseudo-testcross citrus population. We detected 80 flavonoids in this population and identified 138 quantitative trait loci (QTLs) for 57 flavonoids in these four tissues. Based on transcriptional profiling and functional annotation, twenty-one candidate genes were identified, and one gene encoding flavanone 3-hydroxylase (F3H) was functionally verified to result in naturally occurring variation in dihydrokaempferol content through genetic variations in its promoter and coding regions. The abundant data resources collected for diverse citrus germplasms here lay the foundation for complete characterization of the citrus flavonoid biosynthetic pathway and will thereby promote efficient utilization of metabolites in citrus quality improvement.Keywords
Funding Information
- Huazhong Agricultural University Scientific & Techno- logical Self-Innovation Foundation
This publication has 50 references indexed in Scilit:
- Polyphenolic contents in Citrus fruit juices: authenticity assessmentZeitschrift für Lebensmittel-Untersuchung und Forschung, 2014
- Differentiation of lemon essential oil based on volatile and non-volatile fractions with various analytical techniques: a metabolomic approachFood Chemistry, 2014
- Genetic analysis of the metabolome exemplified using a rice populationProceedings of the National Academy of Sciences of the United States of America, 2013
- Genome-wide association mapping of leaf metabolic profiles for dissecting complex traits in maizeProceedings of the National Academy of Sciences of the United States of America, 2012
- An evolutionary view of functional diversity in family 1 glycosyltransferasesThe Plant Journal, 2011
- The Genetic Map of Artemisia annua L. Identifies Loci Affecting Yield of the Antimalarial Drug ArtemisininScience, 2010
- Production of two mandarin × trifoliate orange hybrid populations via embryo rescue with verification by SSR analysisEuphytica, 2007
- Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrusThe Plant Journal, 2004
- Semisynthesis of Linarin, Acacetin, and 6-Iodoapigenin Derivatives from DiosminJournal of Natural Products, 2004
- Regulation of Metabolism in Transgenic PlantsAnnual Review of Plant Physiology and Plant Molecular Biology, 1995