An in vitro cell model to study microglia activation in diabetic retinopathy
- 13 October 2021
- journal article
- research article
- Published by Wiley in Cell Biology International
- Vol. 46 (1), 129-138
- https://doi.org/10.1002/cbin.11710
Abstract
Microglial activation has been studied extensively in diabetic retinopathy. We have previously detected activation and migration of microglia in 8-week-old diabetic rat retinas. It is widely acknowledged that microglia-mediated inflammation contributes to the progression of diabetic retinopathy. However, existing cell models do not explore the role of activated microglia in vitro. In this study, microglia were subject to various conditions mimicking diabetic retinopathy, including high glucose, glyoxal, and hypoxia. Under high glucose or glyoxal treatment, microglia demonstrated only partially functional changes, while under hypoxia, microglia became fully activated showing enlarged cell bodies, enhanced migration and phagocytosis as well as an increased production of pro-inflammatory factors such as COX-2, IL-1β, and iNOS. The data indicate that hypoxia-treated microglia is an optimal in vitro model for exploration of microglia activation in diabetic retinopathy.Keywords
Funding Information
- Natural Science Foundation of Shanghai (20ZR1472600)
- Science and Technology Commission of Shanghai Municipality (18411953400, 19495800700, 20142203200)
- National Natural Science Foundation of China (82171062, 81870667, 81970810, 81970811)
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