Characterization and in vitro assembly of tick‐borne encephalitis virus C protein
Open Access
- 8 June 2020
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 594 (12), 1989-2004
- https://doi.org/10.1002/1873-3468.13857
Abstract
Tick‐borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical–chemical approaches, such as size‐exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid‐like structure with that of dengue C protein.Funding Information
- Grantová Agentura České Republiky (GA18‐27204S)
This publication has 60 references indexed in Scilit:
- Intracellular Nucleic Acid Delivery by the Supercharged Dengue Virus Capsid ProteinPLOS ONE, 2013
- Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface ProteinsJournal of Virology, 2012
- The G-Patch Domain of Mason-Pfizer Monkey Virus Is a Part of Reverse TranscriptaseJournal of Virology, 2012
- In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRVJournal of Virology, 2012
- Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged ProteinACS Chemical Biology, 2010
- Effect of Dimerizing Domains and Basic Residues on In Vitro and In Vivo Assembly of Mason-Pfizer Monkey Virus and Human Immunodeficiency VirusJournal of Virology, 2010
- Structural analysis of viral nucleocapsids by subtraction of partial projectionsJournal of Structural Biology, 2007
- Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactionsNature Protocols, 2006
- Distinct Roles for Nucleic Acid in In Vitro Assembly of Purified Mason-Pfizer Monkey Virus CANC ProteinsJournal of Virology, 2006
- FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATIONAnnual Review of Microbiology, 1990