Rejuvenation Research

Journal Information
ISSN / EISSN: 15491684 / 15578577
Total articles ≅ 1,517

Latest articles in this journal

Ms. Hui-Han Ma, Ms. Jun-Ru Wen, Hao Fang, Ms. Shan Su, Ms. Can Wan, Ms. Chao Zhang, Ms. Fang-Mei Lu, Ms. Ling-Ling Fan, Guang-Liang Wu, Ms. Zi-Yi Zhou, et al.
Published: 3 February 2023
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0054

Abstract:
Ischemia stroke is thought to be one of vascular risks associated with neurodegenerative diseases, such as Alzheimer's disease (AD). Hydroxysafflor yellow A (HSYA) has been reported to protect against stroke and AD, while the underlying mechanism remains unclear. In this study, SH-SY5Y cell model treated with oxygen glucose deprivation/reperfusion (OGD/R) was used to explore the potential mechanism of HSYA. Results from CCK-8 showed that 10μM HSYA restored the cell viability after OGD 2 h/R 24 h. HSYA reduced the levels of malondialdehyde and ROS, while improved the levels of superoxide dismutase and glutathione peroxidase. Furthermore, apoptosis was inhibited and the expression of brain-derived neurotrophic factor was improved after HSYA treatment. In addition, the expression levels of amyloid-β peptides (Aβ) and BACE1 were decreased by HSYA, as well as the expression levels of binding immunoglobulin heavy chain protein, PKR-like ER kinase pathway and activating transcription factor 6 pathway, while the expression level of protein disulfide isomerase was increased. Based on these results, HSYA might reduce Aβ toxicity after OGD/R by interfering with apoptosis, oxidation, and neurotrophic factors, as well as relieving endoplasmic reticulum stress.
Jitendra Kumar Arya, Raushan Kumar, Ms. Akanksha Singh, Ms. Parisha Srivastava, Arun Kumar Yadawa, Syed Ibrahim Rizvi
Published: 27 January 2023
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0032

Abstract:
Increasing age is the single largest risk factor for a variety of chronic illnesses. As a result, the capability to target the aging process has the potential to lead to increased health span. A lack of appropriate glucoregulatory control is a recurring issue associated with aging and chronic illness, even though many longevity therapies result in the preservation of glucoregulatory control. In this study, we suggest that targeting glucose metabolism to improve regulatory control can help to slow the aging process. Male Wistar rats, both young (age 4 months) and old (age 24 months), were given Acarbose (30mg/kg b.w.) for 6 weeks. An array of oxidative stress indicators was assessed after the treatment period, including plasma antioxidant capacity as determined by the ferric reducing ability of plasma (FRAP), reactive oxygen species (ROS), lipid peroxidation (MDA), reduced glutathione (GSH), total plasma thiol (SH), plasma membrane redox system (PMRS), protein carbonyl (PCO), advanced oxidation protein products (AOPPs), advanced glycation end products (AGEs), and sialic acid (SA) in control and treated groups. When compared to controls, acarbose administration increased FRAP, GSH, SH, and PMRS activities in both age groups. The treated groups, on the other hand, showed substantial decreases in ROS, MDA, PCO, AOPP, AGE, and SA levels. The effect of Acarbose on almost all parameters was more evident in old-aged rats. Acarbose significantly increased PMRS activity in young rats, here the effect was less prominent in old rats. Our data supports the restoration of antioxidant levels in older rats following short-term Acarbose treatment. The findings corroborate the potential role of Acarbose as a putative CRM.
Ms. Lijuan He, Ms. Xiaojun Jin, Hui Liu
Published: 21 January 2023
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0052

Abstract:
Objective: This study aimed to establish a complement-tolerance test as a marker of protein fragility and discuss its clinical significance. Methods: Total complement activity (TCA) of serum was measured using a self-hemolysis colorimetric method. Human O-erythrocytes and rabbit anti-human O-erythrocyte antibodies were used to replace sheep erythrocytes and the corresponding hemolysin for the hemolysis test, respectively. The antigen-antibody specific binding activated the classical pathway of complement, generating a membrane attack complex (MAC), and the red blood cells rupture. A complement-tolerance test (CTT) was established to measure complement heat tolerance according to the sensitivity of complement proteins to temperature, which was calculated according to differences in TCA at different temperatures. The smaller the CTT, the stronger the complement resistance to heat. The method was applied to the detection of diabetic patients and healthy controls. Results: The mean value of CTT (Mean) = 0.063 ± 0.003 with a coefficient of variation of 4.8% for the same specimen tested for complementary thermal resistance on five consecutive days, which is a good stability of the assy. Application of CTT on samples from patients with different ages revealed significantly higher mean CTT values for elderly patients (≥60-years old) relative to those for younger patients (20–40-years old) (p < 0.05). Additionally, the mean CTT values for diabetic patients were significantly higher than those for healthy patients (p < 0.001). Conclusion: We successfully established a method that uses complement thermal resistance as a marker of protein fragility, with the results demonstrating the ability of the CTT identify age- and disease-related variations in patient samples and its potential efficacy for clinical application.
Ms. Chaeyeong Kim, Ms. Hye-Min Bae,
Published: 20 January 2023
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0051

Abstract:
Data regarding plant extracts with antiaging properties, particularly via the biological process involving telomeres and telomerase, are limited. Thus, the present study aimed to investigate the effects of Acanthopanax senticosus extract (ASE) supplementation on leukocyte telomere length (LTL), telomerase, and inflammatory and metabolic markers in adult animal models. A freeze-dried product of ethanol extracts was prepared using a mixture product of stem and root ASE. In a 24-week experiment that included 24-week-old Sprague Dawley male rats, experimental rats (n = 10) were administrated with 7 mg/day of ASE dissolved in saline and control rats (n = 10) with saline. All rats had access to chow and tap water ad libitum. Their LTL and plasma levels of telomerase and inflammatory and metabolic markers were assayed and compared between the two groups. The experimental rats showed significantly longer LTL (P-value < 0.05) and lower plasma levels of alanine aminotransferase (P-value < 0.05) and aspartate aminotransferase (P-value = 0.08) compared with the control. In addition, LTL was correlated with the aforementioned biochemical parameters of liver function test among experimental rats only. No significant differences in plasma levels of telomerase and inflammatory and metabolic markers were observed. These findings indicate that ASE supplementation may attenuate LTL shortening and reduce liver biochemical parameters, indicating its potential anti-aging and hepatoprotective effects without any adverse metabolic response.
, Tatsuyuki Ishii, Toru Asou, Kazuo Kishi
Published: 16 January 2023
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0048

Abstract:
Chronic senescence, such as aging, contributes to age-related tissue dysfunction and disease development. The accumulation of senescent fibroblasts and the senescence-associated secretory phenotype are particularly implicated in this process. Removal of senescent cells has been reported to prevent tissue dysfunction and extend life span during aging. ABT-263 (navitoclax), which inhibits antiapoptotic proteins, is a leading antiaging drug; however, its role in human skin aging is unclear. This study aimed to determine the rejuvenating effects of ABT-263 on aging skin using a human skin graft mouse model. We assessed the viability of ABT-263-treated skin fibroblasts after inducing senescence. Aged human skin was transplanted under the back skin of nude mice and injected intraperitoneally with the drug or control. Analysis of the skin specimens revealed that ABT-263 induced selective elimination of senescent dermal fibroblasts. Senescent human skin treated with ABT-263 exhibited a decrease in the number of senescent cells and in the expression of aging-related secretory phenotype molecules, such as matrix metalloproteinases and interleukins and an increase in collagen density. Our results indicate that selective removal of senescent skin cells with ABT-263 can improve the aging phenotype of human skin without side effects. ABT-263 is, thus, a novel potential therapeutic agent for skin aging.
Kento Takaya, Toru Asou, Kazuo Kishi
Published: 26 December 2022
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0056

Abstract:
The current understanding of skin aging is that senescent fibroblasts accumulate within the dermis and subcutaneous fat to cause abnormal tissue remodeling and extracellular matrix dysfunction, triggering a senescence-associated secretory phenotype (SASP). A novel therapeutic approach to prevent skin aging is to specifically eliminate senescent dermal fibroblasts; this requires the identification of specific protein markers for senescent cells. Apolipoprotein D (ApoD) is involved in lipid metabolism and antioxidant responses and is abundantly expressed in tissues affected by age-related diseases such as Alzheimer's disease and atherosclerosis. However, its behavior and role in skin aging remain unclear. In this study, we examined whether ApoD functions as a marker of aging using human dermal fibroblast aging models. In cellular senescence models induced via replicative aging and ionizing radiation exposure, ApoD expression was upregulated at the gene and protein levels and correlated with senescence-associated β-galactosidase activity and the decreased uptake of the proliferation marker BrdU, which was concomitant with the upregulation of SASP genes. Furthermore, ApoD-positive cells were found to be more abundant in the aging human dermis using fluorescence flow cytometry. These results suggest that ApoD is a potential clinical marker for identifying aging dermal fibroblasts.
Mei Yang, Yi Liu, Yuxia Ma, Wenhao Wang
Published: 14 December 2022
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0050

Abstract:
Acute pulmonary thromboembolism (APTE) has become a non-negligible clinical concern due to its high mortality and complex symptoms. Early diagnosis and prognostic assessment of APTE are of great significance for the long-term benefits of patients, especially elderly patients. Elderly patients with pulmonary embolism (n=250) who presented to our hospital from January 2018 to July 2021 were recruited into this study. In addition, 50 healthy elderly people with no history of allergies were selected as the control group. An enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentrations of D-dimer and signal peptide-CUB-EGF domain-containing protein-1 (SCUBE1) in their plasma. Right ventricular volume contraction time (ICT), ejection time (ET), isovolumic relaxation time (IRT) were determined by Doppler ultrasound. Right ventricular Tei-index=(ICT+IRT)/ET. High plasma D-dimer, plasma SCUBE1, and right ventricular Tei index are risk factors for poor prognosis in APTE patients after treatment. Plasma D-dimer, plasma SCUBE1 and right ventricular Tei index have predictive value for poor prognosis in APTE patients. Their combined detection (0.256*DD + 0.04*SCUBE1 + 10.188*Tei) can improve the sensitivity and specificity of prediction. There is a predictive value of combined plasma D-Dimer, SECUBE1 and right ventricular Tei-index for the prognosis of elderly patients with APTE.
Alla I. Potapovich, Tatyana V. Kostyuk, Tatyana V. Shman, Tatyana I. Ermilova, Tatyana G. Shutava,
Published: 6 December 2022
Rejuvenation Research; https://doi.org/10.1089/rej.2022.0031

Abstract:
The work investigated effects of plant polyphenolic compounds (PPs) on responses of cultured human HaCaT keratinocytes to ultraviolet radiation in the C range (UV-C). The experimental data obtained indicate a cytoprotective effect of the PPs added immediately after UV-C exposure. The efficiency of PPs was lowered in the order: acacetin ≥ silybin > quercetin. It was also studied the influence of PPs on phosphorylation of histones H2AX and the number of single-strand DNA breaks in the nuclei of keratinocytes. Using the comet-assay and gamma H2AX staining followed by fluorescence microscopy it has been established that PPs can reduce DNA damage in the nuclei of keratinocytes exposed to UV-C radiation. It is concluded that the PPs can diminish the destructive effect of UV radiation on the skin cells, activating the process of repairing genetic damage.
Xiaojie Liu, Ms. Mengyu Li, Ms. Chen Jian, Ms. Fuxiao Wei, Ms. Huanle Liu, Ke Li, Xue-Mei Qin
Published: 1 December 2022
Rejuvenation Research, Volume 25, pp 275-290; https://doi.org/10.1089/rej.2022.0039

Abstract:
Constipation is one of the most common gastrointestinal disorders, whose incidence increasing with age. As one of the main components, Astragalus polysaccharide (APS) has been used to treat a variety of diseases. This study aimed to explore the effects of APS on the improvement of gastrointestinal function and learning memory in elderly rats with constipation. In this study, both 16S rRNA sequencing-based microbiome and 1NMR-based metabolomics were applied to demonstrate the effects of APS on host metabolism and gut microbiota of the elderly rats with constipation. On top of this, we constructed both inter-and inner-layer networks, intuitively showing the correlations among behavioral indicators, intestinal bacteria, and differential metabolites. Our results showed that APS significantly ameliorated the constipation and the cognitive dysfunctions of rats. Microbiome analysis revealed that APS raised the relative abundance of Blautia wheras decreased the relative abundance of Lactobacillus in the elderly rats with constipation. Additionally, APS decreased the levels of acetate, butyrate, and propionate in the fecal samples, correspondingly regulating glycolysis/gluconeogenesis metabolism and pyruvate metabolism. These findings lay solid foundations for understanding the pathogenesis of constipation in the elderly, and also offer a promising new treatment strategy for constipation in the elderly.
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