Publisher Jurnal Akuakultur Indonesia

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Adang Saputra, Tatag Budiardi, Reza Samsudin, Naufal Dwi Rahmadya
Jurnal Akuakultur Indonesia, Volume 17, pp 104-112; doi:10.19027/jai.17.2.104-112

Abstract:Snakehead Channa striata is a local specific fish species and has high economic value. Until now the production of snakehead still reelies on the catch of nature because cultivation of snakehead is still underdeveloped. The main constraint in snakehead fish farming is high mortality on snakehead juvenile rearing phase. This study was conducted to determine the best stocking density on snakehead juvenile rearing to achieve optimal production. The treatments used in this study were stocking density of 1 juvenile/L, 2 juveniles/L, and 3 juveniles/L. Snakehead juveniles with a length of 3.41 ± 0.39 cm and weight 0.28 ± 0.07 g, were reared for 42 days in the aquarium sized 40×40×40 cm with a volume of 40 L. Fishes were fed by bloodworms in ad libitum method. The result showed that the treatments did not affect the survival, growth and the ratio of RNA/DNA of snakehead juvenile. Survival of juvenile snakehead ranged 92.5‒94.58% (P>0.05). The result of water quality measurement showed that it was on optimum condition to supporting snakehead growth at 3 juveniles/L stocking density. Furthermore, recirculation can be use to maintenance water quality for optimum condition. Thus, the rearing of snakehead fish juvenile in the recirculation system can use a stocking density of 3 juveniles/L, and the recirculation system could maintain the water quality in good condition. Keywords: growth, recirculation system, snakehead fish, stocking density, survival rate ABSTRAK Ikan gabus Channa striata merupakan ikan spesifik lokal dan mempunyai nilai ekonomis tinggi. Sampai saat ini produksi ikan gabus masih mengandalkan tangkapan dari alam karena kegiatan budidaya ikan gabus masih belum banyak berkembang. Kendala utama dalam budidaya ikan gabus adalah tingginya mortalitas pada fase pemeliharaan benih. Penelitian ini dilakukan untuk menentukan padat tebar terbaik dalam upaya memperoleh pertumbuhan dan sintasan terbaik. Perlakuan yang digunakan dalam penelitian ini adalah padat tebar 1 ekor/L, 2 ekor/L, dan 3 ekor/L. Benih ikan gabus dengan panjang rata-rata 3,41± 0,39 cm dan bobot rata-rata 0,28 ± 0,07 g dipelihara selama 42 hari di dalam akuarium berukuran 40×40×40 cm dengan volume air 40 L. Benih ikan gabus diberikan pakan berupa cacing sutera secara ad libitum. Hasil penelitian menunjukkan bahwa penggunaan padat penebaran yang berbeda tidak memengaruhi sintasan dan pertumbuhan dan rasio RNA/DNA benih ikan gabus (P>0,05). Sintasan benih ikan gabus pada akhir pemeliharaan berkisar antara 92,5‒94,58%. Hasil pengukuran terhadap kualitas air pada kepadatan 3 ekor/L masih dalam kondisi optimum untuk mendukung pertumbuhan benih ikan gabus sehingga sistem resirkulasi yang digunakan dapat mempertahankan kualitas air dengan baik. Hasil penelitian menunjukkan pemeliharaan benih ikan gabus pada sistem resirkulasi sebaiknya menggunakan padat tebar 3 ekor/L dan sistem resirkulasi dapat mempertahankan kualitas air dalam kondisi baik. Kata kunci: ikan gabus,...
Wellya Wichi Meritha, Muhammad Agus Suprayudi, Juli Ekasari
Jurnal Akuakultur Indonesia, Volume 17, pp 113-119; doi:10.19027/jai.17.2.113-119

Abstract:This study aimed to evaluate the growth performance and resistance to salinity stress of striped catfish juvenile reared in biofloc with different feeding rate (FR). The treatments applied in this study were rearing the fish in biofloc with FR 5% and 8% of biomass per day, and rearing the fish with a FR of 8% per day without biofloc system as the control. The fish with an initial average length of 1.81 ± 0.20 cm were stocked in 9 units of 50 L aquaria with density of 40 ind/aquaria (800 ind/m3) for 15 days rearing period. In biofloc systems, the addition of tapioca as a source of organic carbon was done every day with an estimated C/N ratio of 10. No water exchange was done in biofloc systems, whereas regular water exchange was applied in the control. Results of the experiment showed that survival was not significantly different amongst treatments (P>0.05). However, the specific growth rate of the fish in biofloc system with a FR of 8% per day showed the highest value and was significantly different from other treatments (P<0.05). Fish reared in biofloc system tend to have lower feed conversion ratios (FCRs) than the control. The lowest FCR was found in fish reared in biofloc system with 5% FR and significantly lower than control (P<0.05). Salinity stress test was conducted by soaking 15 juveniles in water with a salinity of 20 g/L for an hour. The survival of fish after salinity stress test were significantly higher for fish reared in bifloc system than control (P<0.05). These data showed that rearing striped catfish juvenile in biofloc system could reduce FCR, increase the growth, and robustness of fish. Keywords: biofloc, feeding rate, growth, salinity stress test, striped catfish ABSTRAKPenelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan ketahanan benih ikan patin terhadap stres salinitas yang dipelihara dalam sistem bioflok dengan tingkat pemberian pakan (FR) berbeda. Perlakuan yang terdapat dalam penelitian ini adalah benih patin yang dipelihara dalam sistem bioflok dengan FR 5% dan 8% per hari, dan pemeliharaan benih dengan FR 8% per hari tanpa penambahan sumber karbon sebagai kontrol. Benih patin dengan panjang rata-rata awal 1,81 ± 0,20 cm dipelihara dalam 9 unit akuarium dengan volume air 50 L dan kepadatan 40 ekor/akuarium (800 ekor/m3) selama 15 hari. Pada sistem bioflok, penambahan tapioka sebagai sumber karbon dilakukan setiap hari dengan C/N 10. Pada sistem bioflok tidak dilakukan pergantian air, sedangkan pada kontrol dilakukan pergantian air. Kelangsungan hidup ikan tidak berbeda nyata antar perlakuan. Namun, tingkat pertumbuhan spesifik ikan dalam sistem bioflok dengan FR 8% per hari menunjukkan nilai tertinggi dan berbeda nyata antar perlakuan (P<0,05). Benih yang dipelihara pada sistem bioflok memiliki rasio konversi pakan (FCR) yang lebih rendah dibandingkan kontrol, namun tidak terdapat perbedaan yang signifikan antara perlakuan bioflok (P>0,05). Uji stres salinitas dilakukan dengan...
Zahidah Zahidah, Yuli Andriani, Yayat Dhahiyat, Isni Nurruhwati, Asep Sahidin, Herman Hamdani, Stephanie Marcelia Victoria
Jurnal Akuakultur Indonesia, Volume 17, pp 130-136; doi:10.19027/jai.17.2.130-136

Abstract:Aquaponics is new aquaculture integrating the technology of fishes and plants due to less availability of land. This study aimed to determine the optimum retention period in the aquaponics farming of sangkuriang catfish (Clarias gariepinus) integrated with water spinach (Ipomoea reptan) to generate the water to support the catfish production. This study conducted in 40 days between May to June 2016 at Ciparanje Fish Hatchery Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The design of this study was completely randomized, with five treatments and three replications. The treatments retention periods were 5, 10, 15, 20 minutes, and control (no water flow). The study showed that the retention period affected water spinach’s inorganic nutrient absorption in sangkuriang catfish farm. The 15 minutes retention period gave the most desirable result, with a flow of 0.072 L/s that reduced 58.83% of nitrate, and 33.32% of ammonia has been produced by fish farming activities. The highest specific growth rate of the sangkuriang catfish obtained in 15 minute retention period of 4.01 % Keywords: aquaponics, sangkuriang catfish, water spinach, retention periods AbstrakAkuaponik adalah teknologi akuakultur baru yang mengintegrasikan ikan dan tanaman karena ketersediaan lahan yang semakin rendah. Penelitian ini bertujuan untuk menentukan periode retensi optimum pada budidaya akuaponik ikan lele sangkuriang (Clarias gariepinus) yang terintegrasi dengan kangkung air (Ipomoea reptan) untuk menghasilkan air yang mendukung produksi lele. Penelitian ini dilakukan selama 40 hari di Laboratorium Budidaya Ikan Ciparanje, Fakultas Perikanan dan Ilmu Kelautan, Universitas Padjadjaran. Penelitian ini menggunakan rancangan acak lengkap, dengan lima perlakuan dan tiga kali ulangan. Perlakuan berupa periode retensi 5, 10, 15, 20 menit, dan kontrol (tidak ada aliran air). Hasil penelitian menunjukkan bahwa periode retensi memengaruhi penyerapan nutrisi anorganik oleh bayam air dalam budidaya ikan lele sangkuriang. Periode retensi 15 menit memberikan hasil yang paling baik, dengan aliran 0,072 L/s mengurangi 58,83% nitrat, dan 33,32% amonia dalam media budidaya ikan. Laju pertumbuhan spesifik ikan lele sangkuriangtertinggi diperoleh pada perlakuan waktu retensi 15 menit, yaitu sebesar 4,01%. Kata kunci: akuaponik, ikan lele sangkuriang, kangkung darat, waktu retensi
Rahim Rahim, Gede Suantika, Harish Muhammad
Jurnal Akuakultur Indonesia, Volume 17, pp 137-146; doi:10.19027/jai.17.2.137-146

Abstract:This research aimed to obtain the performance of super intensive white shrimp rearing using zero water discharge (ZWD) system. This study consisted of four steps, (1) activation and cultivation of nitrifying bacteria, microalgae C. calcitrans and B. megaterium; (2) acclimatization of Pacific white shrimp PL10 with 30 g/L of salinity and decreasing salinity at 2–3 g/L/day; (3) conditioning of ZWD system; (4) white shrimp rearing in 400 L of tank for ten weeks. The experiment used three treatments, (a) shrimp reared without any addition of microbial agent with water exchange conducted every week as much as10–20% of total rearing volume as control (K); (b) ZWD systems with the applications of nitrifying bacteria, (C. calcitrans and B. megaterium) without water discharge (P1); and (c) ZWD system with the application of microalgae C. calcitrans and B. megaterum without water discharge (P2). According to the results, application of nitrifying bacteria, microalgae C. calcitrans and B. megaterium were able to improve the performance of ZWD system performance of white shrimp rearing at low salinity. In addition, the ZWD system was also able to increase the growth rate and survival rate of shrimp when it compared to control. The best rearing performance was found in ZWD system with application of microalgae C. calcitrans and B. megaterium. Keywords: Litopenaeus vannamei, ZWD, low salinity, microalgae, nitrification bacteria. ABSTRAKPenelitian ini bertujuan untuk mengetahui kinerja budidaya udang putih super intensif bersalinitas rendah menggunakan sistem zero water discharge (ZWD). Penelitian ini terbagi dalam tiga, yaitu (1) aktivasi dan kultur bakteri nitrifikasi, mikroalga C. calcitrans dan B. megaterium; (2) aklimatisasi udang putih PL10 salinitas 30 g/L dan penurunan salinitas 2–3 g/L/hari; (3) pengondisian dari sistem ZWD; (4) pemeliharaan udang putih selama 10 minggu di bak bervolume 400 L. Penelitian ini menggunakan tiga perlakuan ; (a) perlakuan kontrol tanpa penambahan mikroba dan pergantian air setiap minggu sebanyak 10–20% (K) ; (b) sistem ZWD dengan bakteri nitrifikasi, mikroalga C. calcitrans dan B. megaterium tanpa pergantian air (P1); (c) sistem ZWD dengan mikroalga C. calcitrans dan B. megaterum tanpa pergantian air (P2). Berdasarkan hasil yang didapat, aplikasi bakteri nitrifikasi, mikroalga C. calcitrans dan B. megaterum mampu meningkatkan kineja sistem ZWD pada budidaya udang putih L. vannamei bersalinitas rendah. Selain itu, aplikasi bakteri nitrifikasi, mikroalga C. calcitrans dan B. megaterum pada sistem ZWD juga mampu meningkatkan laju pertumbuhan dan sintasan udang putih dibanding dengan kontrol. Kinerja pemeliharaan terbaik dijumpai pada sistem ZWD dengan aplikasi mikroalga C. calcitrans dan B. megaterum. Kata kunci: bakteri nitrifikasi, Litopenaeus vannamei, mikroalga, salinitas rendah, ZWD
Dinamella Wahjuningrum, Acep Muhamad Hidayat, Tatag Budiardi
Jurnal Akuakultur Indonesia, Volume 17, pp 94-103; doi:10.19027/jai.17.1.94-103

Abstract:This research aimed to characterize bacteria caused disease in eel Anguilla bicolor bicolor. The research was conducted in two steps. The first step included the isolation and identification of bacteria from the disease infected glass eel (average body length: 5.0±0.5 cm, average weight: 0.5±0.1 g). The observation were colony and cell morphology, physiology, and biochemical characterization of bacteria, hemolysis test, and bacteria identification performed by KIT API 20 E, KIT API 20 Strep, and KIT API 20 Listeria. The second step was Koch’s postulate, tested on healthy elver with an average length of 15.00±0.65 cm and weight of 3.00±0.75 g. The results showed three dominant species of bacteria suspected as a causative agent in eel, namely: Aeromonas hydrophila, Streptococcus agalactiae, and Listeria grayi. Koch’s postulates test proved that the Aeromonas hydrophila and Streptococcus agalactiae were virulent to Anguilla bicolor bicolor. Thus, A.hydrophila and S. agalactiae were disease-causing agent bacteria in eel. Keywords: Anguilla bicolor bicolor, bacteria, A. hydrophila, S. agalactiae ABSTRAKPenelitian ini bertujuan untuk mengkarakterisasi bakteri penyebab penyakit pada ikan sidat Anguilla bicolor bicolor. Penelitian ini dilakukan dalam dua tahap. Tahap pertama meliputi isolasi dan identifikasi bakteri dari ikan sidat kondisi sakit pada stadia glass eel. Ukuran panjang ikan sidat rata-rata 5±0,5 cm dan bobot rata-rata 0,5±0,08 g, pengamatan bentuk morfologi koloni dan morfologi sel, karakterisasi fisiologi, dan biokimia bakteri, serta uji hemolisis, dan identifikasi jenis bakteri dengan KIT API 20 E, KIT API 20 Strep, dan KIT API 20 Listeria. Tahap kedua yaitu uji postulat Koch pada ikan sidat kondisi sehat stadia elver yang berukuran panjang rata-rata 15±0,65 cm dan bobot rata-rata 3±0,75 g. Hasil penelitian diperoleh tiga jenis bakteri dominan yaitu Aeromonas hydrophila, Streptococcus agalactiae, dan Listeria grayi. Uji postulat Koch membuktikan bahwa bakteri A. hydrophila dan S. agalactiae bersifat virulen pada ikan sidat Anguilla bicolor bicolor. Dengan demikian maka bakteri A. hydrophila dan S. agalactiae sebagai bakteri penyebab penyakit pada ikan sidat. Kata kunci: Anguilla bicolor bicolor, bakteri, A. hydrophila, S. agalactiae
Suclyadi Dairun, Mia Setiawati, Muhammad Agus Suprayudi, Nur Bambang Priyo Utomo
Jurnal Akuakultur Indonesia, Volume 17, pp 87-93; doi:10.19027/jai.17.1.87-93

Abstract:Catfish farming has confronted with the problem of slow growth to reach the fillet size and less tender of fish fillets. This study aimed to investigate the effect of cinnamon leaf flour and shrimp head flour in formulated diets on growth of catfish Pangasianodon hypopthalamus. Catfish with an initial weight of 207.04 ± 2.70 g/fish reared in 12 cages with a dimension of 2×1×1.5 m3 (L×W×H) and its fish density was 15 fishes for 60 days. Fish were fed with following experimental diets: (1) Control; (2) cinnamon leaf (1%) (CL); (3) 45% of protein source from shrimp head (SH); (4) CL+SH mix. The fishes fed twice a day with feeding rate 3.5% of fish biomass. The study found that the use of cinnamon leaf and shrimp head increased the specific growth rate 1.67-1.70%, the feed efficiency 57.55-57.67%, and protein retention 55.61% compared to its control (P<0.05). Triglyceride level, cholesterol, and blood HDL were 416.00-524.05 mg/dL, 139.65-156.68 mg/dL, 73.18-103.70 mg/dL (P>0.05), respectively. HSI value ranged between 0.3-1.9% compared to its control (P<0.05).Keywords: Cinnamomum burmannii, feed, growth, Pangasianodon hypopthalmus, shrimp head. ABSTRAKBudidaya ikan patin dihadapkan pada permasalahan pertumbuhan dan kualitas daging yaitu pertumbuhan yang lambat untuk mencapai ukuran fillet serta tekstur daging kurang kompak. Tujuan penelitian ini adalah untuk menguji pengaruh penggunaan tepung daun kayu manis dan tepung kepala udang dalam pakan terhadap pertumbuhan ikan patin Pangasianodon hypopthalmus. Ikan patin dengan bobot 207,04 ± 2,70 g/ekordipelihara selama 60 hari di hapa sebanyak 12 buah (berukuran 2×1×1,5 m3)dengan padat tebar 15 ekor/jaring. Ikan diberi pakan perlakuan yang terdiri dari: (1) Kontrol; (2) daun kayu manis 1% (DKM); (3) 45% sumber protein dari kepala udang (TKU); dan (4) campuran DKM+TKU. Pemberian pakan sebanyak 2 kali sehari dengan feeding rate 3,5% dari biomassa ikan. Hasil dari penelitian penggunaan daun kayu manis dan kepala udang meningkatkan nilai laju pertumbuhan harian 1,67-1,70%, efisiensi pakan 57,55-57,67% dan retensi protein 55,61% dibanding kontrol (P<0,05). Kadar trigliserida, kolesterol, dan HDL darah berturut-turut adalah 416,00-524,05 mg/dL, 139,65-156,68 mg/dL, 73,18-103,70 mg/dL (P>0,05). Nilai HSI ikan patin berkisar antara 0,3-1,9% dibanding kontrol (P<0,05).Kata kunci: Cinnamomum burmannii, kepala udang, pakan, Pangasianodon hypopthalmus, pertumbuhan.
Enita Romasni Turnip, Widanarni Widanarni, Anja Meryandini
Jurnal Akuakultur Indonesia, Volume 17; doi:10.19027/jai.17.1.68-80

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Linuwih Aluh Prastiti, Munti Yuhana, Widanarni Widanarni
Jurnal Akuakultur Indonesia, Volume 17, pp 81-86; doi:10.19027/jai.17.1.81-86

Abstract:This study aimed to evaluate the immune response and growth performance of white shrimp administered with prebiotic mannan oligosaccharides (MOS) with dosages of (0%, 0.2%, 0.4%, and 0.8% in diet) and used in the feeding trial. Shrimps (Litopenaeus vannamei) (the initial average weight was 3.416±0.064 g) were fed at satiation, three times a day. A completely randomized design was used in the study. Shrimps were cultured at the stock density of 15 shrimps 40/L for each treatment in triplicates. After 30 days of the feeding trial, shrimp were challenged with white spot syndrome virus filtrate by intramuscular injection. The total gut bacteria, total haemocyte count (THC), phenoloxydase (PO), and respiratory burst (RB) activity were observed 4 times, before the experiment, day 30th before challenge test, day 32nd after challenge test, and day 36th the end of the experiment. The shrimp survival was observed at day 36th to evaluate the immune responses. The results showed that THC, PO activity, RB activity, growth performance, and shrimp survival administered with prebiotic 0.8% were significantly higher (P<0.05) than control. The administration of prebiotic with dose 0.8% was the best result and could effectively improve the immune responses and growth performance of whiteleg shrimp. Keywords: prebiotic, whiteleg shrimp, white spot disease ABSTRAK Tujuan dari penelitian ini adalah untuk mengevaluasi respons imun dan performa pertumbuhan pada udang vaname yang diberi prebiotik mannan-oligosaccharides (MOS) dengan dosis berbeda (0%, 0,2%, 0,4%, dan 0,8%) pada pakan. Udang vaname (Litopenaeus vannamei) (dengan rata-rata bobot 3,41 ± 0,06 g) diberi pakan tiga kali sehari secara at satiation. Penelitian ini menggunakan rancangan acak lengkap. Udang dipelihara dengan kepadatan 15 ekor per 40/L pada setiap perlakuan dengan tiga kali pengulangan. Setelah 30 hari pemberian pakan, udang diuji tantang menggunakan white spot syndrome virus dengan diinjeksi secara intramuskular. Total bakteri usus, total haemocyte count (THC), aktivitas phenoloxydase (PO), dan aktivitas respiratory burst (RB) diamati 4 kali, yaitu sebelum perlakuan hari ke-30 sebelum uji tantang, hari ke-32 setelah uji tantang, dan hari ke-36 pada akhir penelitian. Hasil penelitian menunjukkan bahwa THC, aktivitas RB, aktivitas PO, performa pertumbuhan, dan kelangsungan hidup yang diberi prebiotik dengan dosis 0,8% lebih tinggi (P<0,05) jika dibandingkan dengan kontrol (dosis 0%). Pemberian prebiotik dengan dosis 0,8% merupakan hasil terbaik dan secara efektif mampu meningkatkan respons imun dan performa pertumbuhan pada udang vaname. Kata kunci: prebiotik, udang vaname, white spot disease
Muliani Muliani, Endang Susianingsih
Jurnal Akuakultur Indonesia, Volume 17, pp 43-52; doi:10.19027/jai.17.1.43-52

Abstract:The experiment aimed to determine the effectiveness of mangrove leaves (Sonneratia alba and Bruguiera gymnorrhiza) extracts for prevention of WSSV infection in black tiger shrimp, Penaeus monodon. Mangrove leaves (S.alba and B. gymnorrhiza) were taken from Maros and Pangkep Regency, respectively. Mangrove leaves dried approximately for two weeks, made into flour, extracted using 80% methanol, and then evaporated. The yield partitioned using two types of solvents, theywere butanol and dietyleter. Anti WSSV activity test was done by injection method with the ratio of mangrove extract and WSSV was 2:1 as much as 100 µL/ind. The treatment were; A). Water fraction of S. alba + WSSV suspention; B). Butanol fraction of S. alba + WSSV suspention; C). Dietyleter fraction of S. alba+ WSSV suspention; D). Methanol extract of S. alba + WSSV suspention; E). Water fraction of B. gymnorrhiza + WSSV suspention; F). Butanol fraction of B. gymnorrhiza + WSSV suspention; G). Dietyleter fraction of B. gymnorrhiza + WSSV suspention; H). Methanol extract of B. gymnorrhiza + WSSV suspention; I). Positive control (WSSV suspention without mangrove extract). Each treatment was repeated three times with 10 days of rearing period. The results showed that the highest activity of anti-WSSV from S. alba was in dietyleter fraction and the methanol extract, while the highest activity of anti-WSSV from B. gymnorrhiza was in butanol fraction. The dietyleter fraction and the methanol extract of S. alba and the butanol fraction of B. gymnorrhiza were capable to stimulate immune response of shrimp and were effective in deactivating WSSV furthermore it increased the black tiger shrimp survival rateto 100%. Keywords: antiviral, white spot syndrome virus (WSSV), mangrove herbs, S. alba, B. gymnorrhiza, Penaeus monodon ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak dari daun mangroveSonneratia. alba dan Bruguiera gymnorrhizauntuk pencegahan infeksi WSSV pada udang windu, Penaeus monodon. Daun mangroveS.alba dan B. gymnorrhiza masing-masing diambil dari Kabupaten Maros dan Kabupaten Pangkep. Daun mangrove dikering anginkan selama dua minggu, dibuat tepung, diekstraksi dengan metanol 80%, dan dievaporasi. Rendemen yang diperoleh dipartisi menggunakan dua jenis pelarut yaitu butanol dan dietileter. Uji aktivitas anti WSSV dilakukan metode penyuntikan dengan perbandingan ekstrak mangrove dengan WSSV 2:1sebanyak 100 µL/ekor. Perlakuan yang diujikan adalah; A). Fraksi air S. alba + suspensi WSSV; B). Fraksi butanol S. alba + suspensi WSSV; C). Fraksi dietileter S. alba+ suspensi WSSV; D). Ekstrak metanol S. alba+ suspensi WSSV; E). Fraksi air B. gymnorrhiza + suspensi WSSV; F). Fraksi butanol B. gymnorrhiza + suspensi WSSV; G). Fraksi dietileter B. gymnorrhiza + suspensi WSSV; H). Ekstrak metanol B. gymnorrhiza + suspensi WSSV; I). Kontrol positif (suspensi WSSV tanpa ekstrak mangrove). Masing-masing perlakuan diulang tiga kali dengan lama pemeliharaan 10 hari....
Rio Yusufi Subhan, Eddy Supriyono, Widanarni Widanarni, Daniel Djokosetiyanto
Jurnal Akuakultur Indonesia, Volume 17, pp 53-60; doi:10.19027/jai.17.1.53-60

Abstract:The aim of this research was to determine optimum stocking density for growing-out of spiny lobster Panulirus sp. in controlled tanks that conducted for 30 days. The experimental spiny lobsters have the initial average weight of 130.39 ± 0.32 g and initial average total length of 140.70 ± 0.06 mm. This study used completely randomized design with three different stocking densities (KT10: 10 ind/m3; KT18: 18 ind/m3; and KT26: 26 ind/m3) and two replications. The parameters observed in this study included water quality (temperature, pH, salinity, dissolved oxygen, and total ammonia nitrogen), physiological responses (total haemocyte count, haemolymph glucose, and frequency of molt), and production performances, such as growth, specific growth rate, feed conversion ratio, and survival rate. The results showed that the spiny lobster could be reared in high stocking density in controlled tanks. Water quality during the study in each treatment was; temperature 26.56–28.65oC, salinity 29.7–33.6 g/L, pH 7.5–8.5, dissolved oxygen 6.15–6.58 mg/L, and total ammonia nitrogen 0.11–0.34 mg/L. The best stocking densities for spiny lobster was 18 ind/m3 (KT18) with 2.5‒3.5×106cells/mL total haemocyte counts, 24.6‒28.3 mg/dL haemolymph glucose, and 38.37 ± 3.20% frequency of molt. The final average body weight and length were 145.06 ± 0.42 g and 142.77 ± 0.19 mm, respectively. The survival rate reached 86.11 ± 3.92% with a specific growth rate 0.35 ± 0.01%/day, and feed conversion ratio 7.87 ± 0.31.Keywords: high stocking density, Panulirus sp., physiological responses, productivity. ABSTRAKTujuan dari penelitian ini adalah untuk menentukan kepadatan terbaik dalam pembesaran lobster laut Panulirus sp. yang dipelihara dalam bak terkontrol selama 30 hari. Lobster laut yang digunakan pada awal penelitian memiliki bobot 130,39 ± 0,32 g dan panjang total 140,70 ± 0,06 mm. Penelitian dilakukan menggunakan rancangan acak lengkap dengan tiga perlakuan kepadatan berbeda, yaitu: 10 ekor/m3(KT10), 18 ekor/m3(KT18), dan 26 ekor/m3(KT26) dan dua ulangan. Parameter uji yang diamati dalam penelitian ini meliputi kualitas air (suhu, salinitas, pH, DO, dan TAN), respons fisiologis (total hemosit/THC, glukosa hemolim, dan frekuensi pergantian kulit), dan kinerja produksi meliputi pertumbuhan, laju pertumbuhan spesifik, rasio konversi pakan, dan tingkat kelangsungan hidup. Hasil penelitian menunjukkan bahwa lobster laut dapat dibesarkan dengan kepadatan tinggi dalam bak terkontrol. Pengukuran nilai kualitas air pada setiap perlakuan selama pemeliharaan adalah suhu berkisar 26,56–28,65oC, salinitas 29,7–33,6 g/L, pH 7,5–8,5, DO antara 6,15–6,58 mg/L dan TAN antara 0,11–034 mg/L. Perlakuan terbaik selama penelitian adalah dengan kepadatan 18 ekor/m3 (KT18) dengan nilai THC berkisar antara 2,5–3,5×106 sel/mL, glukosa hemolim 24,6–28,3 mg/dL,dan frekuensi pergantian kulit 38,37±3,20%. Bobot dan panjang lobster akhir rata-rata pada perlakuan...
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