Journal Journal of Life Sciences Research

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Memis Özdemir, Zeynep Sönmez, Mehmet Topal
Journal of Life Sciences Research, Volume 5, pp 1-5; doi:10.20448/journal.504.2018.51.1.5

Abstract:The aim of the study was to determine the gene and genotype frequencies of the bGH/AluI and CSN3/HinfI gene in the animal material obtained from organic farm by PCR-RFLP method. A total of 248 cattle of Holstein breed were genotyped for the bGH/AluI and CSN3 HinfI polymorphism. In bGH gene region, the LL, LV and VV genotype frequencies were 0.50, 0.48 and 0.02 respectively and In CSN3 gene region, the AA, AB and BB genotype frequencies were 0.71, 0.23 and 0.06 respectively. Both bGH/AluI and CSN3/HinfI genotypes were not found to be in equilibrium within the breed. Also, both heterozygosity were found at a high rate as 0.478 and 0.234 and the calculated FIS values were -0.24 and 0.20 respectively.
Jin-Hua Ouyang, Hong-Zhen Li
Journal of Life Sciences Research, Volume 4, pp 14-19; doi:10.20448/journal.504.2017.42.14.19

Md. Ashrafuzzaman Gulandaz, Abu Sayed, M Nur-A- Alam, M.A. Hossain, Mahedi Hasan, Mashiur Rahman
Journal of Life Sciences Research, Volume 4, pp 5-13; doi:10.20448/journal.504.2017.41.5.13

Shola K. Babatunde, Deboye O. Kolawole, Majekodunmi R. Adedayo, Adeyinka E. Ajiboye, Abudllahai T. Ajao, Omolara N. Mustapha
Journal of Life Sciences Research, Volume 4, pp 1-4; doi:10.20448/journal.504.2017.41.1.4

Etta H. E., Okon E. A.
Journal of Life Sciences Research, Volume 3, pp 44-49; doi:10.20448/journal.504/2016.3.4/504.3.44.49

Abstract:A breeding investigation was carried out to evaluate the effect of Phyllanthus amarus (Schonn. and Thonn.) on the phenotypic characters of hatchlings of Giant African Land Snails (GALS), Achachatina marginata. The phenotypic traits of one hundred (100) hatchlings investigated were live (body) weight (BW), shell mouth length (SML), shell mouth width (SMW), shell length (SL), shell width (SW) and Feed Intake (FI). The snails were randomly assigned to treatment groups A(10%), B(20%), C (30%) inclusion of Phyllanthus amarus powdered leaves into the normal chow of the hatchlings and D (control), with twenty-five (25) snails in each group. 5 weeks after commencement of treatment, the live (body) weights (BW) and shell length (SL) of the snails showed significant differences (p0.05) The feed intake was highly significant (p<0.01) in all the groups. From the results obtained, P. amarus leaf meal was well tolerated by the hatchlings and is recommended to snail breeders, as a feed supplement for A. marginata hatchlings bred for commercial purposes.
Iskandar Mavlyanov, Abdurashid Ashirmetov, Zafar Mavlyanov, Bekhzod Abdullaev
Journal of Life Sciences Research, Volume 3, pp 50-56; doi:10.20448/journal.504/2016.3.4/504.3.50.56

Xin- Ming Cao, Ting Liu, Qing- Ping Hu
Journal of Life Sciences Research, Volume 3, pp 40-43; doi:10.20448/journal.504/2016.3.3/504.3.40.43

Abstract:The oil from clove buds had a wide range of pharmacological action and bioactivities and is widely used in the medicine, food and flavouring industries. In this work, the antibacterial activity of the essential oil (EO) and oleoresin (OL) from clove buds against several food-related bacteria were evaluated based on Oxford cup method, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays, and the results showed that there is no obvious difference in antibacterial activity between essential oil and oleoresin, and they exhibited better antibacterial activity against test bacteria, and the antibacterial effects depended on its concentrations and action time. Kill-time assay also showed the essential oil and oleoresin from clove buds had a significant inhibitory effect on the growth rate of surviving Staphylococcus aureus and Escherichia coli.
Nemat Olimov, Zaynab Sidametova
Journal of Life Sciences Research, Volume 3, pp 34-39; doi:10.20448/journal.504/2016.3.3/504.3.34.39

Abstract:Background. Distress, due to pain, fear/anxiety, dyspnea, or delirium is common among critically ill patients. Distress may manifest clinically as agitation that is often associated with ventilator asynchrony and vital sign abnormalities. Regardless, distress needs to be treated to comfort the patient, ameliorate agitation that may interfere with supportive care, and attenuate increases in sympathetic tone, which may have untoward physiological effects. Purpose. Common sedative-analgesic medications used to treat distress in critically ill adults. Identifying the cause of distress and using this information to select the optimal sedative-analgesic agent is discussed separately. The aim of the study was to evaluate effectiveness of the new sedative drug complex “Flegmen” in preclinical study. Materials and Methods. The elemental composition of sedative assemblage "Flegmen" was analyzed by the method of mass spectrometry induction coupled plasma on the instrument ICP-MS 7500a AT the company "Agilent Technologies". Sedative assemblage "Flegmen" contains 39 elements. In the greatest number of macrocells in the sedative assemblage "Flegmen" contains chlorine, phosphorous, bromine, sodium, chromium, titanium, zinc and strontium. Also set the content of calcium (9.6 µg), which relieves the consequences of stress. Amino acid composition of sedative collection was studied analyzer. Identification of amino acids of the collection and their quantitative content in nanomolar was calculated according to the integrator, which is supplied with amino acid analyzer. Clinical studies were performed in the Republican Specialized Scientific-Practical Center (RSSPC) of Therapy and Medical Rehabilitation, the Department of Neurology, Departments of GP-therapy, Clinical Allergology, Propedeutics of Internal Diseases, Hematology, and Professional Diseases in the Tashkent Medical Academy. Experiments and Results. The study included 90 patients who received drug, which is studied, and 60 patients received the drug comparison on outpatient and inpatient care. Clinical study was divided three particular clinical trials. Patients were divided into two groups: basic and control. Mean age was 44.5±3.61 year. According to evaluation of the effectiveness of the drug "Flegmen" was found extremely tolerability in all 90 (100%) patients. Side effects were not revealed in the research. Conclusion. It was confirmed that sedative assemblage "Flegmen" is the most effective at treatment of patients with various diseases followed by an asteno-neurotic syndrome and neuroses that allows to recommend it for use in practical health care.
I. Bushara, M.O. Mudalal, Dafalla M. Mekki, S.A. Babhker, ElGazafey Besheer
Journal of Life Sciences Research, Volume 3, pp 30-33; doi:10.20448/journal.504/2016.3.2/504.2.30.33

Abstract:The experiment was conducted on desert male goats to evaluate the effect feeding regimen and castration on body measurements and their relationship between carcass measurements, slaughter weight and hot carcass weight. Seventy two (72) male desert goats aged 4-5 months and weighing 12.1 Kg (11.7-12.4 Kg) were used in these experiments. Goats were divided randomly into three groups; each group (24 males) was divided into two sub groups (12 males) in Factorial experimental randomized design. The results revealed that nutrition had significant (p<0.01) effect on all body measurements (body length, belly girth, heart girth, height at wither, width of hips and height at rump), on the other hand castration and interaction of (feeding regimen and castration) had significant (p<0.01) effect on total change in body length and total change in height at wither. Correlation coefficients between slaughter weight and body measurements were significant in all feeding regime except heart girth and belly girth in grazing with supplementation and zero grazing goats respectively were significant. All correlation coefficients between hot carcass weight and body measurements were significant in all feeding regime except the wither height in free grazing goats and wither height and rump height in zero grazing goats were significant.DOI: 10.20448/journal.504/2016.3.2/504.2.30.33
Moksia Froumsia, Louis Zapfack, Pierre Marie Mapongmetsem, Bernard-Aloys Nkongmeneck
Journal of Life Sciences Research, Volume 3, pp 18-29; doi:10.20448/journal.504/2016.3.2/504.2.18.29

Abstract:The study focused on fuel wood economic value and the influences of woody cutting on woody species in the flooded plains woodland area. Sahelian woodland suffers from cutting down trees for fuel wood, to insure household energy demands. Investigations were carried out near a sample of users of resources and an inventory of stems of exploited species in woodland. Households in 15 villages and four markets were explored and 496 actors included in the exploitation and the use of firewood and charcoal, as regard 204 men and 292 women, were interviewed individually. Fuel wood quantity and economic value was made through a register. Through 48 transects of 2 000 m length and 20 m each, the availability, the intensity of cutting down trees, measurements and observations were made and noted on stems. Stems which the circumference > 10 cm were counted and their diameter was measured. The sources of domestic energy mostly used were firewood and charcoal which remained easily accessible and available. Completely stem cut down and partial stems cut were noted as the exploitation mode. The charcoal was produced traditionally, using furnace. Significant quantity of firewood (2186.59 t) and charcoal (28340 t) were estimated. It varied periodically a year with a substantial drop. The quantity sale represent 95 % and brought annual income of 122035.8 $ (Firewood) and 31 630 $ (charcoal). The incomes contributed significantly in the rural households’ economy and brought an additive to their much reduced agricultural yields. A number of 33 woody species were noted as the mostly exploited for fuel wood. Among them Anogeissus leiocarpus, Balanites aegyptiaca, Prosopis Africana, Detarium microcarpum and Pseudocedrela kotschyi were firstly mention. The total number of stumps and the partial cut of stems were significant representing respectively 20.91 % and 9.64 % of stems counted. The exploitation was intensive and selective on stems. Cutting down of stems was constituted a major factor for requested species degradation. Results indicated evidence of the impacts of fuel wood exploitation on the woody species. The study concludes that fuel wood yield in the sahelian woodland has not yet reached alarming proportions and can be contained. It could be an imperative to set up an alternative system to ensure sustainable management of resources. Solutions through introduction and popularization of natural gas, biogas, solar energy and the improved hearths could effectively contribute to reduce the intensity of this activity and to guarantee the maintenance of the ecological balance on the already fragile sahelian ecosystems.DOI: 10.20448/journal.504/2016.3.2/504.2.18.29
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