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Journal Jurnal Ilmu Ternak dan Veteriner

190 articles
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Endang Tri Margawati, Slamet Diah Volkandari, Indriawati Indriawati, Muhamad Ridwan
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 168-173; doi:10.14334/jitv.v23i4.1915

Abstract:Bali cattle is one of local beef cattle in Indonesia, up to present its performance indicated an inbreeding occurrence. This study was aimed to analyze the genetic diversity and relationship among Bali cattle from several locations in Indonesia based on ETH10 microsatellite marker. Ninety-four (94) DNA samples (89 Bali cattle; 5 Banteng) were analyzed. The Bali cattle samples were from 6 locations in Indonesia (15 Pulukan; 15 Nusa Penida; 14 Bima West Nusa Tenggara/WNT; 10 Mataram, WNT; 20 Riau; 15 South Borneo). DNA Banteng samples were collected from Prigen Malang of East Java. Microsatellite marker of ETH10 labelled HEX was used for amplification. Alleles were analyzed by using Cervus 3.0.7 and GenAlex 6.5. Result showed that there were five (5) alleles found in ETH10 marker i.e., 209; 213; 215; 217; and 219 bp. Average of observed (Ho) and expected (He) heterozygosity were 0.46±0.05 and 0.60±0.03, respectively. Five (5) out of 6 locations were in breeding occurrence except Bali cattle from Mataram was not inbreeding. The longest genetic relationship was between Bali cattle from Mataram and Riau whereas the closest distance was Bali cattle from South Borneo with Mataram. Banteng was closest to Bali cattle from Nusa Penida and the longest was to Bali cattle from South Borneo. This finding indicates there is inbreeding in Bali cattle, therefore it needs to be concerned in bull rotation and semen distribution for increasing the Bali cattle performance.
A. A. Affan, Fma Amirul, Aaa Ghani, S Annas, M Zamri-Saad, Ha Hassim
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 180-188; doi:10.14334/jitv.v23i4.1922

Abstract:Pregnancy ketosis has been recognized as one of the common metabolic disease affecting goat’s meat and milk production. For the present study, sixteen (n=16) individuals of pregnant does at day 80 of pregnancy had been used. A total of 8 does were categorized as control group (healthy pregnant goats), were fed on Napier grass and goat concentrate with water ad libitum, and another 8 does were considered as treatment group which categorized as ketosis based on the clinical signs and presence of ketone body in urine. Blood sample were collected from all goats for biochemical profiles analysis which were glucose, Beta-hydroxybutyrate (BHBA), free fatty acid (FFA), calcium, electrolytes (sodium, potassium, chloride), liver enzyme and hormonal levels (cortisol and insulin). Three does from each group were slaughtered and liver samples were collected for fatty acid profiles study. In this study, the BHBA, FFA, calcium, amino aspartate transferase (AST), gamma glutamyl transferase (GGT) and cortisol hormone were significantly higher in pregnancy ketosis goats as compared to control group. Meanwhile, the concentration of glucose, sodium, potassium, chloride and insulin hormones were lower in pregnancy ketosis goats as compared to control. Furthermore, the fatty acid composition in blood plasma of pregnant goat with ketosis showed higher level of palmitic, stearic and oleic acid, while in liver, palmitic, oleic and linoleic acid was found higher.
Harapin Hafid, A Napirah, Sm Sarifu, Rahman, Inderawati, Nuraini, Hasnudi
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 202-209; doi:10.14334/jitv.v23i4.1914

Abstract:This research was aimed to study the effect of electrical stimulation period on physical and organoleptic properties of Muscovy duck meat. This research used 20 female Muscovy ducks, 1.5-2 years of age. The ducks were divided into 5 groups treatments for 4 replications. The treatments were period of electrical stimulation: 0, 5, 10, 15, and 20 minutes. The result showed that period of electrical stimulation did not affect (P>0.05) cooking loss but significantly affected (P<0.05) the tenderness, color, flavour, aroma, pH, and juiciness of duck meat. The best treatment was 20 minutes stimulation.
Eslam Faid-Allah
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 159-167; doi:10.14334/jitv.v23i4.1845

Abstract:This study was carried out to evaluate the sires and dams genetically for milk production and mastitis traits in Egyptian 12 herds of Holstein cattle using Best Linear Unbiased Prediction via MTDFREML program. The data was obtained from a commercial farm called Dena, located in Cairo-Alex Desert Road (80 Km), Menofia, Egypt. Data included 4791 cows, 4227 dams and 248 sires that represented the period from 2007 to 2014. Estimating breeding values for milk production traits as cumulative milk yield at 90 days (90-DM), cumulative milk yield at 180 days (180-DM), cumulative milk yield at 270 days (270-DM), cumulative milk yield at 305 days (305-DM), and number of mastitis infection around the season of lactation (MAST). The averages of the 90-DM, 180-DM, 270-DM, 305-DM and MAST were 3026.3±655.1 kg, 5873.3±1081.1 kg, 7891.1±2692.2 kg, 9611.2±1897.9 kg, and 0.712±1.2 time/parity, respectively. Estimates of heritability for the previous traits were 0.11±0.016, 0.15±0.014, 0.18±0.012, 0.22±0.015, and 0.09±0.029, respectively; genetic variance were 47206.2 kg, 175300.6 kg, 1304654.4 kg, 792411.6 kg and 0.12 time/parity, respectively; and phenotypic variance were 429147.6 kg, 1168670.6 kg, 7248079.9 kg, 3601870.9 kg, and 1.35 time/parity, respectively. The EBV values as average, SD, (Min: Max) for sires were 0.0±0.179 (-0.4: 0.66) for MAST, 0.0±86.176 (-263.1: 245.4) for 90-DM, 0.0±227.523 (-600.3: 800.3) for 180-DM, 0.0±413.48 (-323.3: 1277.7) for 270-DM and 0.0±440.26 (-1280.9: 1565.1) for 305-DM. Also, The EBVs for dams were 0.0±0.055 (-0.14: 0.45) for MAST, 0.033±26.24 (-142.8: 103.0) for 90-DM, 0.074±76.81 (-360.2: 289.6) for 180-DM, -0.045±139.66 (-591.9: 529.2) for 270-DM and 0.266±154.1 (-666.3: 617.6) for 305-DM. These results provide that the selection of sires and dams will improve the traits of milk production and mastitis in this herd because of the wide differences in genetic potential among sires and dams.
Widaningrum, Miskiyah, D Indrasti, Hc Hidaya
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 189-201; doi:10.14334/jitv.v23i4.1547

Abstract:Lactobacillus casei and Bifidobacterium longum are probiotics commonly applied as dry starter for food system. Drying process in the production of dry starter can reduce the number of probiotics, therefore they are necessary to be encapsulated. Aim of this research was to obtain best encapsulating material for both probiotics. Encapsulation technique used in this research was extrusion with maltodextrine-alginate, sago starch-alginate, corn starch-alginate, and control of treatment skim milk-alginate (w:w) as encapsulating materials. The four encapsulating materials significantly affected the value of viability, encapsulation efficiency, number of cell in wet beads and dry beads, number of survival cell during drying process, and number of survival encapsulated cell in simulated acid and bile salt conditions. Based on viability of L. casei and B. longum, beads matrix characteristic, number of cell in wet beads and dry beads, and number of survival cells during drying process, maltodextrine-alginate was better than sago starch-alginate and corn starch-alginate, but was not as good as skim milk-alginate (control of treatment) as encapsulating material. Viability (number) of L. casei and B. longum in dry beads of maltodextrine-alginate were 4.69±0.08 log CFU/g and 5.32±0.21 log CFU/g, while number of L. casei and B. longum in dry beads of skim milk-alginate were higher 5.08±0.07 log CFU/g and 6.20±0.16 log CFU/g. L. casei more resistant than B. longum against acidic (low pH) environment. In the presence of 0.3% bile salt, L. casei and B. longum encapsulated with skim milk-alginate increased as much as 2.75±0. 02 and 1.61±0.04 log cycles, respectively.
Widya Pintaka Bayu Putra, St Nugraheni, Y Irnidayanti, S Said
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 174-179; doi:10.14334/jitv.v23i4.1862

Abstract:Gen Insulin-like Growth Factor 1 (IGF1) pada mamalia berfungsi untuk mengontrol pertumbuhan tulang dan otot. Oleh karena itu gen IGF1 banyak digunakan sebagai salah satu kandidat gen untuk seleksi ternak. Penelitian ini bertujuan untuk mengidentifikasi genotip gen IGF1 (ekson 1) menggunakan metode PCR-RFLP dengan enzim restriksi SnaBI (TAC*GTA). Sebanyak 90 ekor sampel DNA sapi Pasundan dari Kabupaten Ciamis dan Pangandaran, Jawa Barat telah digunakan pada penelitian ini. Hasil penelitian ini menunjukkan bahwa seluruh sampel yang dianalisis memiliki genotip CC dengan alel C sebagai alel yang umum pada gen IGF1/SnaBI. Genotip CC yang diperoleh pada penelitan ini disebabkan karena terdapat mutasi transisi pada posisi basa ke g.218T/C (GenBank: KF202095). Mutasi ini menyebabkan perubahan asam amino dari methionine (AUG) menjadi valine (GUG). Disimpulkan bahwa gen IGF1/SnaBI pada sapi Pasundan bersifat monomorfis dan tidak dapat digunakan untuk seleksi molekuler.
A. P. Pertiwi, L.I.T. A. Tumbelaka, M. F. Ulum
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 130-142; doi:10.14334/jitv.v23i3.1809

Abstract:Ultrasonography is a diagnostic method to image the conditions of reproductive organs and it could be supported by vaginal cytology to identify the activities of the ovaries by the types of vaginal exfoliate cells. The aims of this study was to observe reproduction organ through ultrasonography with supportive diagnostic with vaginal cytological assessment. A total of 10 individual queens were used in this study and then grouped into intact group (n=5) and spayed (ovariohysterectomy) group (n=5) based on the anamneses or their medical history. The vagina, cervix, uterus body and horns, and ovaries were imaged and measured by ultrasound. Vagina, uterine body and horn seem as pipe-like structures with hyperechoic outer lines. The lumen in uterine body and horn seem as a hyperechoic structure. The ovaries seem as round- or oval-shaped structures with anechoic follicles. The corpus luteal has thick wall and seen as anechoic in its centre part. The corpus albicans seems as a hyperechoic structure. The vagina of spayed queens seemed more corrugated than those intact queens. The cervix is seen as a hyperechoic structure linking the vagina and uterine body. Exfoliate vaginal epithelial cell types were then also be identified and counted on each queens. The results of vaginal cytology showed that proestrus occured in 3 intact queens, late metestrus in 1 intact and 3 spayed queens, anestrus in 1 spayed queen, and unidentifiable estrus stage in 1 intact and 1 spayed queens. Moreover, the morphology of cervix and uterine was affected by the activity of ovary.
T. Pasaribu, A. P. Sinurat, E. Wina, T. Purwadaria, T. Haryati, I.W. R. Susana
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 112-122; doi:10.14334/jitv.v23i3.1851

Abstract:The use of antibiotic growth promoters (AGP) has been banned as feed additives in many countries, therefore the alternatives need to be found. An in vitro experiment was conducted to study the potential of combination of some plant extract to inhibit growth of pathogen bateria that normally occur in the poultry gastro intestinal tract.The combination of three plants bioactives (liquid smoke of cashew shells of Anacardium occidentaleor CLS, Phyllanthus niruri L. extract (EM), and Synzygium aromaticum extract (EDC) were formulated and evaluated for its effectiveness to inhibit growth of Escherichia coli and Salmonella sp. in vitro.The mixtures (KE) were then made in 3 different concentrations, i.e. 100 % KE, 50% KE, and 25% KE and studied their effectiveness to inhibit growth of E. coli or Salmonella sp. using microplate reader method. The results showed that the higher the concentration of the bioactive combination (KE100) the higher the ability to inhibit the growth of E. coli or Salmonella sp. The combination of bioactive substances CAM, EM, and EDC more effectively than Zn-bacitracin antibiotics to inhibit the growth of E. coli and Salmonella sp. The optimum concentration of KE with the similar effectiveness as the AGP was 25%. It was concluded that the combination of CAM, EM, and EDC was able to inhibit the growth of E. coli and even capable to eliminate the presence of Salmonella sp. In the biological assay, a combination of CAM, EM, and EDC either extract or powder form, high dose, medium or low does not affect the weight of the spleen, bursa fabricius, and blood profile. The best combination of CAM, EM, and EDC extracts to decreases the total bacterial population and E. coli was middle dose ie 0.0625% (extract) and 0.625% (powder). Likewise for live weight gain that was a good dose of extract or powder form can replace antibiotics. It was concluded that combinations of CAM, EM, and EDC had potential as a substitute for AGP in poultry feed, especially chickens.
R. Widiastuti, Y. Anastasia
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 143-149; doi:10.14334/jitv.v23i3.1789

Abstract:This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small dairyl farms. A total of 104 samples of fresh cow's milk were collected in Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed by a high performance liquid chromatography and detected with fluorescence detector after extraction with organic solvents. Contamination of AFM1 was found on 1.96% (1/51) from the samples collected in April 2012 at concentration of 1.20 ng/L and 39.63% (21/53) from the samples collected in September 2012 at concentration of 1.20 ng/L 1.0 – 34.1 ng/L. Those positive samples were obtained from Pangalengan and Sukabumi, but none for those samples collected from Tanggamus both on collection time April nor September 2012. In those positive samples for AFM1, there is no sample contained AFM1 above the maximum level (ML) regulated in Indonesia (500 ng/L or 0.5 µg/L). Low contamination levels of AFB1 in the range of 0.38 to 6.64 µg/kg found in supplemental feed samples from the same sampling time and locations. The findings of AFM1 contamination in raw fresh milk from this study caused no harm to the consumers. However, regular monitoring on the presence of AFM1 in dairy milk and aflatoxin B1 (AFB1) in dairy cattle feed is necessary to ensure the protection of human health.
Gustian, H. Soehartono, N. Jujur, A.H. S. Wargadipura, D. Noviana
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 123-129; doi:10.14334/jitv.v23i3.1836

Abstract:This study was aimed to obtain information regarding complete blood count (CBC) profile of post implantation of stainless steel (SS) 316L as an Indonesian local product of non-degradable metal implant. Thirty adult male rat, aged approximately 12 weeks were divided into 3 groups, i.e. control group without implantation, implant group with import SS316L and implant group with Indonesian national local SS316L that developed by Agency for the Assessment and Application of Technology (BPPT). The implant groups were given implants by inserting it between femoral bone and biceps femoris muscle. On the control group, defect was made on bone without inserting an implants material. Examination of the systemic response was done with CBC before and 30 days after implantation. The analysis of red blood cells amount, haemoglobin level, haematocrite value, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell and its differentiation from each group did not show significant differences. In conclusion, stainless steel 316L of import and national local product showed non-negative effects on blood profile.
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