Journal Visnyk of Dnipropetrovsk University. Biology, medicine

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V. Y. Kalynovskyi, A. S. Pustovalov, G. Y. Grodzyuk, N. S. Andriushyna, M. E. Dzerzhynsky
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 106-111; doi:10.15421/021619

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V. Y. Gasso, A. M. Hahut, S. V. Yermolenko
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 127-131; doi:10.15421/021623

Abstract:The grass snake Natrix natrix (Linnaeus, 1758) is a partly hygrophilous species, distributed throughoutUkraine. This snake may be considered as a test object for environmental biomonitoring. Modern biochemical methods make it possible to obtain new scientific data on the effects of anthropogenic pressure on reptiles. Blood is a sensitive and informative indicator of the condition of an organism as it responds quickly to most changes in exogenous and endogenous factors, and reflects negative influences on both individual and, indirectly, populations. Changes in biochemical parameters may be used as biomarkers of the state of health of reptiles in ecosystems under varying degrees of anthropogenic pressure. Due the increase in anthropogenic influence the development and introduction of new methods of perceptual research, collection of up-to-date information and development of a database of reptile biochemical parameters have become an urgent priority. We collected mature individuals of the grass snake in floodplain ecosystems on the right bank of the Dnieper River in Dnipropetrovsk city. Grass snakes from floodplain habitats on the left bank of theSamaraRiver (O.L. Belgard Prysamarskii International Biosphere Station, Novomoskovsk district, Dnipropetrovsk province) were studied as the control specimens. Our study demonstrated statistically significant differences between snakes from the study sites in the amount of albumin, urea and urea nitrogen, and inorganic phosphorus, as well as in alanine aminotransferase (ALT) and alkaline phosphatise (AP) activity. The amount of albumin in the blood serum of specimens from the anthropogenically transformed areas was significantly lower (by 25%) than in that of the snakes caught in the control habitats. Decrease of the albumin concentration usually indicates abnormal processes in the kidneys and liver. According to the changes observed in the concentration of albumin, a corresponding increase in the albumin to globulin ratio by about 30% was found. A statistically significant increase in alkaline phosphatase activity may demonstrate negative physiological changes in tissues of the liver and bones. Increased activities of ALT and AP indicate certain changes in the physiological condition of the liver of snakes from the anthropogenically transformed site. Other studied parameters did not exceed the limits found for different species of reptiles. Significant differences between certain biochemical parameters in the serum of snakes from the studied regions may reflect pathological processes in the grass snakes from transformed ecosystems, mainly in their liver and kidneys. The results can be used in future studies as benchmarks for assessing the condition of the organism of this species in the conditions of anthropogenic stress.
M. I. Hariv, B. V. Gutyj
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 123-126; doi:10.15421/021622

Abstract:This article presents the results of research into the influence of the complex liposomal preparation Butaintervit on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning. Intramuscular injection of carbon tetrachloride into rats at a dose of 0.25 ml per100 gof body weight causes antigenic load on the body and leads to disruption of protein synthesis function in the liver. This is shown by reduction in blood levels of total protein and its fractions. Thus, the level of albumin in the serum of rats under the conditions of oxidative stress was 70% lower than in clinically healthy animals. However, the level of total protein in the serum was only 10% lower. This is because, along with the decrease of albumin content in the serum, the levels of globulin protein fraction increased by 8.8%. This has led to albumin/globulin disparities in the serum of sick animals. As a result, the value of A/G coefficient was 0.28 ± 0.03, compared to 0.52 ±0.02 inclinically healthy rats. For the normalization of functional state of the liver under oxidative stress it is advisable to apply the liposomal preparation Butaintervite, which in its structure contains butafosfan, interferon, thistle and vitamins A, D and E. Under conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group we have found significant increase in the levels of total protein and albumins and a decrease in serum globulin in the animals on the fifth and tenth days of the experiment. On the fourteenth day of the experiment under the conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group the normalization of protein synthesis function in the liver was observed. The level of indicators of total protein, albumin, globulin and the coefficient of albumin/globulin compared with the control group of animals were within normal values.
A. G. Hurieva, J. V. Semerak, A. S. Anatsky
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 101-105; doi:10.15421/021618

Abstract:The necessity has been widely noted of improving the nutritional value of forage mixtures used in the poultry industry through the use of enzyme preparations on the basis of phytase for splitting phytic acid and its salts contained in the feed. This technological approach is relevant in feeding farm animals, particularly poultry, as they are unable to synthesise endogenous phytase. On the base of the Agro-Industrial Complex "Oril-Leader" we researched the influence of the enzyme preparation Ladozim Proxy as a feed component for broiler chickens and laying hens on the growth and biochemical parameters of the birds and their egg-laying capacity. The feed ration consisted of wheat, corn, sunflower meal, extruded soy and protein-vitamin complex Biomix. For the research experimental and control groups of broiler chickens were formed (50 birds in each group) and laying hens (20 birds in each group). The experimental feeding period was 35 days for the chickens and 30 days for the hens. The research used physical-chemical methods for determining the content of nitrogen, calcium and phosphorus in the feed, the blood and the excrement of the birds. Statistical data processing was carried out by ANOVA. It was established that the use of Ladozim Proxy in the amount of 60–100 mg/kg feed promotes an increase of the live weight of chickens by 21%. The findings can be explained by an increase of the feed nutrients available for digestion in the gastrointestinal tracts of the poultry due to the hydrolytic action of the enzyme complex added to the phytic-containing components of the feed. The result of these phenomena is also a positive dynamic in assimilation of nitrogen, calcium and phosphorus in the feed by chickens of the experimental group: use of these elements increased by 10–16%. The preparation had no effect on hematological indices of the organisms of the poultry and increased the amount of calcium and phosphorus in the blood. The use of the preparation has not only physiological, but also economic importance because it allows a reduction in feed intake and expenditure on feed for poultry reared for agricultural production. The use of the phytase preparation does not affect the quantitative indicators of egg-laying by hens, but an increase in the thickness of the egg shells was noted, due to deposition of calcium in the organism of the experimental group.
R. Yanko, V. Berezovsky, M. Levashov, L. Plotnikova, O. Chaka
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 90-95; doi:10.15421/021616

Abstract:The aim of the study was to investigate and compare the effect of exogenous melatonin administration on the morphofunctional state of the exocrine and endocrine parts of the pancreases of spontaneously-hypertensive rats (SHR line) in spring and autumn. The research was conducted on 48 male SHR line rats, age 4 months. The rats of the experimental group received melatonin (Unipharm Inc.,USA) every day (at 10 am) orally at a dose of 5 mg/kg of body weight. The duration of the experiment was 28 days. Morphofunctional activity of the exocrine and endocrine parts of the pancreas was assessed using morphological and morphometric methods. The histological preparations were made by the standard methods. The slides were photographed using a digital camera on a Nikon microscope. The morphometric analysis was performed on digital images using the computer program Image J. The morphological signs of the exocrine pancreas activity were greater in the control rats in spring compared to autumn. Conversely, the activity of the endocrine part of the pancreas in control animals was higher in autumn, than in spring. Exocrine pancreas activity increases after administration of melatonin regardless of time of the year. This was evidenced by the increase in size and height of the epithelial acini, and the number of exocrinocytes placed in them. The width of the interlobular and interacinus connective tissue layers in the pancreas decreased in spring (mostly) and in autumn. The activity of the endocrine part of the pancreas increased after administration of melatonin in spring. Proof of this was an increase in the number and size of islets of Langerhans and the number of endocrinocytes they contained, compared with control values. The number and size of islets of Langerhans, the number and density of the endocrinocytes they contained decreased in the rats which received melatonin in autumn. This indicates a reduction of the endocrine pancreas activity. These results may have implications for therapeutic dosing of melatonin in patients with hypertension in different seasons of the year.
R. F. Aminov, A. K. Frolov
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 96-100; doi:10.15421/021617

Abstract:The nitroblue tetrazolium test reflects the degree of activation of oxygen-dependent mechanisms of bactericidal activity of phagocytotic cells, so the increase in nbt-test and phagocytic indices shows increased activation in the bacterial process and enhanced protection with the help of the absorption ability of the cells in the body. We examined females of nonlinear rats after feeding of their offspring, which 2 weeks before and 2 weeks after mating were injected intraperitoneally with antigens of saline extract of medical leeches (4 injections) and the offspring at days 1, 15, 30, 45, 60. The dosage of salt extract antigens was carried out by determining the protein concentrations using the Lowry protein assay. The animals were immobilized using an immobilizing device. The animals were distributed into three groups: the first research and experimental group of animals was subject to the influence of medical leech salt extract antigens to the amount of 0.5 ml (at the rate of 3 µg/g of animal weight); the second group of intact animals was not subject to intervention, the third control group of animals was administered intraperitoneally saline 0.5 ml of 9% saline solution. We studied females after feeding offspring and offspring at 1, 15, 30, 60 days. The animals were kept in vivarium conditions in individual cages on a standard diet. In the experiment we used 60 females of non-linear rats and 300 of their offspring. All animals were decapitated under ether anesthesia. After that, we took blood, made a solution with crystal heparin and examined the laboratory parameters: total number of leukocytes and leukocyte formula. Evaluation of nonspecific resistance of the organism was investigated using tests which characterize the activity of neutrophils (nbt-test, phagocytosis indices). The results of our studies revealed a positive effect of antigens of saline extract of medical leeches on the absorptive and metabolic activity of neutrophils in the females and their offspring in all study periods compared with the control, an increased number of leukocytes, due to the change of the process of differentiation and deposition of cells in immunogenic organs on proliferation. Changes in immunological parameters indicate the immunostimulatory effect of AG ML on leukopoiesis and on the functional activity of neutrophils – the first line of defense against entry into the body of a variety of bacteria, fungi and protozoa.
T. Ashcheulova, N. Gerasimchuk
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 118-122; doi:10.15421/021621

The publisher has not yet granted permission to display this abstract.
N. M. Palibroda
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 83-89; doi:10.15421/021615

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U. V. Tymoshenko, S. O. Sivkovych, K. G. Garkava, R. P. Pavlyuk, G. A. Myronenko
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 112-117; doi:10.15421/021620

Abstract:We analyzed the frequency distribution of blood groups AB0, Rhesus and Kell in patients with B-cell non-Hodgkin lymphoma. An increase of D− patients (31.0%) among ill in comparison to healthy individuals (16.5%) was found. Based on published data, we exclude weakening or alteration of the D antigen for this pathology. We found an association of B-cell non-Hodgkin lymphoma and D− phenotype. It is established that the chances of developing the disease in D− individuals is 2 times higher than for D+ individuals. However, we did not find a significant association of frequency of minor antigens Rhesus (C, c, E, e, CW), K antigen Kell system, A and B antigens AB0 system and the development of B-cell non-Hodgkin lymphoma. We have analyzed the statistics of incidence of non-Hodgkin lymphoma inWestern Europeand Asian countries. We noticed a roughly twofold increase in the incidence of non-Hodgkin lymphoma inWestern Europecompared to Asian countries, where the proportion of D− individuals is almost zero. Parallel with the results of our study, it has been found that the chances of D– persons developing B-cell non-Hodgkin lymphoma are twice as high as for D+ individuals. InUkraine,BelarusandRussiathe incidence of non-Hodgkin lymphoma is almost at the level of Asian countries even though the percentage of D– individuals corresponds to Western European countries (15–16%). This can be explained by other genetic factors, or reduction in quality of care and diagnostics in post-Soviet countries compared toWestern Europe. Thus, D antigen may have a protective role in the morbidity of non-Hodgkin lymphoma.
N. V. Didyc
Visnyk of Dnipropetrovsk University. Biology, medicine, Volume 7, pp 73-77; doi:10.15421/021613

Abstract:The significant incidence of hypertensive disease among the Ukrainian population makes the search for new methods of management and treatment of patients suffering from this pathology a matter of high priority. Our research is focused on study of changes of some biochemical parameters in patients suffering from hypertensive disease as well as patients suffering from hypertensive disease and coexistent extrasystoles. Arterial hypertension leads to different structural and functional changes in the cardiovascular system. In many patients these changes may result in rhythm disorders. 120 patients have been examined (42 men and 78 women) between the ages of 27 to 81 (average 59.8) suffering from hypertensive disease of the II stage as defined by the Ukrainian Association of Cardiologists and from extrasystoles (more than 30 episodes per hour of research) of different topography and 30 persons (13 men and 17 women) suffering from hypertensive disease of the II stage between the ages 30 to 76 (average 56.4) without rhythm disturbance). The first clinical group was formed by 54 (45%) patients suffering from repeated supraventricular extrasystoles (SE) (average 62 ± 2 SE per hour of research). The second clinical group was formed by 42 (35%) patients suffering from repeated ventricular arhythmia (VA) (average 37 ± 2 VA per hour of research). The third group was formed by 24 (20%) patients suffering from both repeated SE and VA (average 48 ± 3 SE and 32 ± 3 VA per hour of research). We conducted 24 hours’ monitoring of blood pressure and electrocardiography using the “Dia Card”-system (“Solvaig”, Ukraine) for all patients. This revealed that ventricle extrasystoles and combined extrasystoles in patients with essential hypertension, II stage are associated with more unfavourable changes in 24 hours’ profile of blood pressure. The paper analyses the cardiac arhythmia structure of heart rate variability in patients with arterial hypertension (AH) and extrasystoles. The findings confirmed the presence of desynchronization in the activity of the neurohnmoral system. Thus, different mechanisms of arhythmogenesis were revealed in patients with arterial hypertension. In the results, frequent ventricular and combined extrasystoles were associated with an unfavourable blood pressure profile during 24-hours monitoring as well as higher end-diastolic left ventricular volume, diastolic dysfunction, aortic valve fibrosis, aorta remodeling, endothelial dysfunction and more prominent lipid disorders. Supraventricular extrasystoles were associated with hypertriglyceridemia, left ventricular diastolic dysfunction and left atrium enlargement.
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