Journal Information
ISSN / EISSN : 0147-619X / 1095-9890
Published by: Elsevier BV (10.1016)
Total articles ≅ 2,588
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Latest articles in this journal

, Karmen Jeseničnik, Lauren P. Elam, Andrej Kastrin, Luka Predojević,
Published: 22 January 2022
The publisher has not yet granted permission to display this abstract.
Semiramis Castro-Jaimes, Gabriela Guerrero, Elena Bello-López,
Published: 22 December 2021
The publisher has not yet granted permission to display this abstract.
, Alexandra Helleux, Sören Abel
Published: 18 November 2021
Abstract:
We developed a simplified, highly efficient Gateway reaction that recombines target DNA to expression (destination) plasmids in vivo and subsequently conjugates the final vector into a recipient strain, all in a single step. This recipient strain does not need to contain any selective marker and can be freely chosen as long as it is sensitive to ccdB counterselection and can be targeted by the RP4α conjugation system. Our protocol is simple, robust, and cost effective. It works in 96-well plate format and performs across a range of temperatures. We designed modular, minimal destination vectors containing a modified Gateway insert to ease vector design by providing locations for insertion of tags, promoters, or conjugations. To demonstrate the utility of our system, we created destination vectors with split adenylate cyclase tags for bacterial two-hybrid (B2H) studies and screened a library of diguanylate cyclases for protein-protein interactions in a single step.
Daniel Pérez-García, Violeta Larios-Serrato, Rogelio Rojas-Rios, Jorge Erick Otero-Olarra, Itza Mendoza-Sanchez, ,
Published: 17 November 2021
The publisher has not yet granted permission to display this abstract.
Li Liu, Yue Chen, Shiqin Yu, Jian Chen,
Published: 10 July 2021
The publisher has not yet granted permission to display this abstract.
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