Cell Biology International Reports

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ISSN : 0309-1651
Published by: Elsevier BV (10.1016)
Total articles ≅ 3,629
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H Aström
Published: 1 September 1992
Cell Biology International Reports, Volume 16, pp 871-81

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Published: 1 August 1992
Cell Biology International Reports, Volume 16

R Van Driel, A Verkleij
Published: 1 August 1992
Cell Biology International Reports, Volume 16

P Creti, A Capasso, M Grasso, E Parisi
Cell Biology International Reports, Volume 16, pp 427-432; https://doi.org/10.1016/s0309-1651(06)80062-7

Abstract:
The adenylate cyclase system present in particulate fractions prepared from two planarian species was tested for sensitivity to various neurotransmitters. While dopamine and other catecholamines were ineffective, serotonin was capable of stimulating the enzyme. Among the various serotonin agonists tested, only 8-OH-DPAT resulted effective on the adenylate cyclase activity, thus suggesting the presence in planarians of a serotonin receptor of the type 5-HT1A.
S Harrishooker, G Sanford, V Montgomery, R Rivers, N Emmett
Cell Biology International Reports, Volume 16, pp 433-450; https://doi.org/10.1016/s0309-1651(06)80063-9

Abstract:
Low density lipoproteins (LDL) are thought to play a major role in cardiovascular diseases such as atherosclerosis. Much remains to be done to understand the cellular effects of LDL and how the extracellular matrix (ECM) influences these effects. We found that LDL produced a dose dependent increase in vascular smooth muscle cell (SMC) proliferation. The ECM altered the proliferative response of SMC to LDL: on collagen I there was a 66% inhibition, endothelial cell derived-ECM a 2-fold increase, and collagen IV no difference in proliferation compared to paired controls. LDL affected SMC motility (cell area and shape factor) but the extent and direction of the effect depended on whether the cells were cultured on uncoated or coated dishes. LDL treated cultures had a 5-fold lower migration rate but net movement was not different, suggesting that LDL decreased SMC random movement. There was a dose-dependent accumulation of lipid by SMC incubated with LDL and, subsequently, cytoplasmic lipid droplets were observed. Cells cultured on uncoated plates showed an increased cholesterol content as a function of LDL concentration. In contrast, cells cultured on a collagen IV matrix showed no net change in cholesterol content over the range of LDL concentrations studied. Hence, the uptake of LDL cholesterol appears to be completely inhibited by this matrix. These studies indicate that the influence of LDL on several SMC parameters is modulated by ECM components.
C Hunt, H Stebbings
Cell Biology International Reports, Volume 16, pp 465-474; https://doi.org/10.1016/s0309-1651(06)80065-2

Abstract:
Results presented here indicate that the high molecular weight microtubule-associated proteins isolated from the ovaries of the hemipterans, Oncopeltus fasciatus and Notonecta glauca, while absent from nervous tissue, are present in the testes of the respective species. Here they are seen to be located to extensive microtubule aggregates within the cells surrounding the sperm, but not obviously to the sperm themselves.
M Bilej, J Rejnek, L Tuckova
Cell Biology International Reports, Volume 16, pp 481-485; https://doi.org/10.1016/s0309-1651(06)80067-6

Abstract:
The Staphylococcal protein A (SpA) binding protein was detected on the surface of annelid coelomocytes. The flow cytometric analysis revealed that 50% coelomocytes of Lumbricus terrestris react with SpA, a figure six times higher than the number of positive coelomocytes found in Eisenia foetida.
M Leclerc, M Bajelan
Cell Biology International Reports, Volume 16, pp 487-490; https://doi.org/10.1016/s0309-1651(06)80068-8

Abstract:
The axial organ (A.O. cells) of the sea star Asterias rubens is a primitive immune organ. The total population was fractionated into two populations: adherent (B-like) and non-adherent (T-like) to nylon wool. The adherent cells resemble mammalian B lymphocytes and bear homologous human T cell receptor (beta chain) to a higher degree than T-like cells which resemble T lymphocytes.
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