Jurnal Teknologi Laboratorium
ISSN / EISSN : 2338-5634 / 2580-0191
Published by: Poltekkes Kemenkes Yogyakarta (10.29238)
Total articles ≅ 54
Latest articles in this journal
Jurnal Teknologi Laboratorium, Volume 9, pp 159-167; https://doi.org/10.29238/teknolabjournal.v9i2.247
This research is to investigate the parameters which may affect the mobilization of stem cells in patients receiving autologous hematopoietic peripheral blood stem cell transplantation (PBSCT). A retrospective study was carried out using the data derived from the medical files of 242 patients who received PBSCT. Descriptive, clinical, and laboratory parameters were compared between patients with successful and unsuccessful stem cell mobilization. Successful stem cell mobilization ratio was 4.463 times higher when preemptive plerixafor was administrated; 1.032 times higher when CD34+ cell count increased 1 unit at the beginning of mobilization. The white blood cell count was inversely correlated with the success of mobilization. An increase of 1 unit in WBC count was associated with a 1.027 times decrease in the success rate. The data indicated that the administration of preemptive plerixafor and CD34+ cell count at the beginning of mobilization were directly related to the success of mobilization after PBSCT. On contrary, WBC count was inversely associated with the success rate.
Jurnal Teknologi Laboratorium, Volume 9, pp 186-191; https://doi.org/10.29238/teknolabjournal.v9i2.235
T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.
Jurnal Teknologi Laboratorium, Volume 9, pp 145-150; https://doi.org/10.29238/teknolabjournal.v9i2.258
Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL.
Jurnal Teknologi Laboratorium, Volume 9, pp 128-135; https://doi.org/10.29238/teknolabjournal.v9i2.237
Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.
Jurnal Teknologi Laboratorium, Volume 9, pp 151-158; https://doi.org/10.29238/teknolabjournal.v9i2.213
Erythrocyte index can form erythrocyte morphology in the peripheral blood smear preparation. Lead exposure in Tambaklorok exceeds the threshold that affects anemia. The purpose of this study was to determine the erythrocyte index confirmation with erythrocyte morphology in the peripheral blood smear preparation. A cross-sectional study was conducted in 2month in Tambaklorok Semarang residents and 104 samples were taken using the purposive technique. Erythrocyte index was measured using the hematological analyzer and erythrocyte morphology in the peripheral blood smear preparation using Giemsa painting. Erythrocyte index confirmation with the peripheral blood smear preparation was analyzed using the Gamma statistical relationship test. The results showed that the erythrocyte index value was mostly in the normal category, i.e., MCH 68 (64.4%), MCHC 61 (58.6%) and MCH 58 (56%) and below normal category were MCH 45 (42.95%), MCHC 41 (39.4%), and MCV 36 (34.3%). Erythrocyte morphology was mostly hypochromic, namely 46 (44.23%), normochrome 40 (38.46%), and hyperchrome 18 (17.3%). Relationship of MCH with erythrocyte color p-value 0.037 with size p-value 0.038. Conclusion of erythrocyte index confirmation, especially MCH with the peripheral blood smear preparation, there was a match on the color and size of erythrocytes, while the MCV and MCHC values had no significant relationship.
Jurnal Teknologi Laboratorium, Volume 9, pp 168-175; https://doi.org/10.29238/teknolabjournal.v9i2.204
Congenital toxoplasmosis can cause damage and death to the fetus, to prevent this case, toxoplasmosis testing is important for the woman of childbearing age. One of the methods to screening the presence of T. gondii in the blood is Polymerase Chain Reaction (PCR). One of the T. gondii genes which can be used as a marker is the B1 gene. There are many toxoplasmosis cases in Indonesia, but the data is still difficult to find in West Bandung Regency. This study aimed to determine the number of toxoplasmosis cases in a woman of childbearing age in West Bandung Regency using the B1 gene as a marker and to determine the factors that influence these cases by conducting statistical analysis on the results of the questionnaire. The sample used in this study was 50 women of childbearing age (got married and domiciled in West Bandung). All samples have met the inclusion criteria and signed the informed consent. DNA from blood specimens was isolated using the Wizard Genomic DNA Purification Kit. The concentration and purity of isolated DNA were measured using a nanodrop device. Besides, the B1 gene from T. gondii was amplified using a pair of specific primers and visualized by the agarose electrophoresis method. Data were analyzed using the logistic regression method. The results showed that 7 women of childbearing age women (14%) in West Bandung Regency had toxoplasmosis. Frequent contact with pets, especially cats, was a significant factor (p
Jurnal Teknologi Laboratorium, Volume 9, pp 192-198; https://doi.org/10.29238/teknolabjournal.v9i2.243
The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining. The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of
Jurnal Teknologi Laboratorium, Volume 9, pp 176-185; https://doi.org/10.29238/teknolabjournal.v9i2.228
Abroma augusta L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, A. augusta is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of A. augusta L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of A. augusta was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH3, CH2, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based A. augusta.
Jurnal Teknologi Laboratorium, Volume 9, pp 136-144; https://doi.org/10.29238/teknolabjournal.v9i2.207
Grass-jelly is one of the most popular plants consumed by people in various forms. Contamination can cause various diseases, one of those is typhoid fever by Salmonella typhi. The purpose of this study was to detect S. typhi in grass-jelly based on molecular detection by amplification of the fliC gene using PCR. Validation was done by culture methods on SSA media and biochemical testing. The fliC gene amplification results in grass-jelly samples (A1, A2, B1, B2, C1, and C3) showed the DNA fragments size of about 1500 bp. Colony and biochemical characters isolate Peterongan were lead to S. typhi, whereas another isolate was another Salmonella spp. Grass-jelly samples from the Peterongan market in Semarang were positively contaminated by S. typhi and isolate from Pedurungan and the minimarket was another Salmonella spp. Molecular-based food testing is fast enough and accurate for detecting types of bacterial contaminants but the amplification of only the fliC gene cannot specific for S. typhi.
Jurnal Teknologi Laboratorium, Volume 9, pp 103-114; https://doi.org/10.29238/teknolabjournal.v9i1.230
Coronaviruses (COVS) are viruses transmitted through droplets of sputum from an infected person. Analyses identify COVS as zoonotic pathogens, possibly resulting from human-animal contact at animal markets. They share overlapping genetic characteristics with the avian influenza viruses from China. COVS released from humans through droplets of sputum and may land on various surfaces, which poses exposure risks; as studies have shown the virus can exist intact for a relatively long period of time (several days). The recent highly pathogenic COVS outbreak (COVID-19) emerged in Wuhan, China in 2019, include Severe Acute Respiratory Syndrome (SARS-COVS). This highly transmittable disease causes pneumonia and severe respiratory illnesses similar to SARS and MERS; it has a global mortality rate of about 6.13%. The virus has rapidly become a global pandemic, causing major global issues, including health, economic, and age-preference, among other issues. This text summarizes the nature of the emerging COVID-19 global pandemic while analyzing several factors concerning the etiology of the virus. This is done in an urgent effort to educate and provide relevant information about the virus.