Dental Journal (Majalah Kedokteran Gigi)
ISSN / EISSN : 19783728 / 24429740
Current Publisher: universitas airlangga (10.20473)
Total articles ≅ 636
Latest articles in this journal
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 57-60; doi:10.20473/j.djmkg.v52.i2.p57-60
Abstract:Background: Erbium chromium or erbium lasers constitute preferred instruments for the preparation of that section of tooth adjacent to the pulp chamber rather than high-speed drills, especially in cases of dental injury. Their advantages can support modified operations in achieving optimum recovery and avoiding complications related to the healing process. Purpose: The aim of the study was to describe another modality of the assistance of erbium, chromium:yattrium-scandium-gallium-garnet (Er,Cr:YSGG) laser in traumarelated pulp injury affecting the anterior teeth. Case(s): The results of laser treatment applied to four pulp injuries of three children were analyzed in this case report. Case Management: Er,Cr:YSGG laser-assisted cavity preparation was performed without resort to a local anesthetic, the cavities being sealed with mineral trioxide aggregate (MTA). None of the cases demonstrated symptomatic or peri-radicular pathology during clinical or radiographic examinations. Conclusion: The results contained in this case report support the application in pediatric dentistry of a Er,Cr:YSGG laser to pulpotomy cavities as part of the treatment of traumatic pulpal injuries to permanent incisors.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 95-99; doi:10.20473/j.djmkg.v52.i2.p95-99
Abstract:Background: Loss of tooth structure is a consideration in the performance of restorative treatment involving nanofilled composite resins. Material polymerization factors and water absorption can affect the hardness of composite resins. Imperfect polymerization producing an oxygen inhibited layer (OIL) and causing water absorption can even compromise the hardness of nanofilled composite resins. Tamarind soft drink, on the other hand, has an acidic pH that compromises the hardness of nanofilled composite resins. Purpose: This study aimed to reveal the effects of glycerin application on the hardness of nanofilled composite resins immersed in tamarind soft drinks. Methods: The research constituted a laboratory experiment using 24 nanofilled composite resin samples with diameters of 5mm or 2mm, divided into six groups, namely: Group G, Group G AS 60, Group G AS 120, Group TG, Group TG AS 60, and Group TG AS 120. Glycerin was applied to the surfaces of three groups before curing, while the other three groups were not treated with glycerin. Finishing was subsequently conducted on all samples using a highspeed handpiece and superfine finishing bur, before they were polished with a low speed handpiece. The samples were then divided into specific groups, namely: a group with a 120-minute immersion time, a group with a 60-minute immersion time, and a group which was not immersed and maintained at a temperature of 37oC. Each sample was tested at three points using a Vickers hardness tester (VHT). Results: The results showed that the groups with glycerin had a higher hardness level than those groups. In addition, the non-immersed groups had a higher hardness level than those groups which were immersed. The one-way ANOVA test results confirmed that there was a statistically significant difference (p
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 90-94; doi:10.20473/j.djmkg.v52.i2.p90-94
Abstract:Background: Trauma-induced ulcers constitute one of the painful abnormalities affecting the oral cavity about which numerous individuals complain. The prevalence of traumatic ulcers (15-30% of cases) is relatively high compared to other oral lesions. Unfortunately, the use of anti-inflammatory drugs classified as steroids can lead to contraindications and serious side effects. Hence, the use of natural ingredients represents an alternative treatment. One such ingredient is red pomegranate containing bioactive elements acting as anti-inflammatory, antibacterial, antimicrobial, and antioxidant agents which accelerate the healing process in traumatic ulcers. Purpose: The aim of this study was to determine the effects of the topical application of red pomegranate (Punica granatum Linn) extract on the healing process in traumatic ulcers in Wistar rats. Methods: 24 male rats, aged 2-3 months, were divided into six groups, namely; two control groups (PC: triamcinolone acetonide and NC: sterile aquades) and four treatment groups (red pomegranate extract gel at respective concentrations of 12.5%, 25%, 50% and 75%). A traumatic ulcer was subsequently created using a flame-shaped burnisher tip 2mm in diameter and heated for ±15 seconds. The diameter traumatic ulcer of each research subject was measured and observed until it healed within the remedial parameters determined by means of a UNC-15 periodontal probe which had been placed on it. The data obtained was analyzed using Kruskal-Wallis and Mann-Whitney tests. Results: Topically applied red pomegranate extract gel can reduce the width of the ulcerated area, while also accelerating traumatic ulcer healing. There was a significant difference in the healing time between the group using red pomegranate extract gel at a concentration of 75% and the positive control group using 0.1% triamcinolone acetonide with p=0.44 (p
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 66-70; doi:10.20473/j.djmkg.v52.i2.p66-70
Abstract:Background: Many Iraqi children of high socioeconomic status (SES) families attend dental clinics presenting predominantly cavitated and painful multiple carious lesions. The factors responsible for dental caries within this sector of society need to be identified. Purpose: The aim of this study is to assess the dental care behavior of mothers and its relationship with the prevalence of dental caries in pre-school age children drawn from high SES families in northern Iraq. Methods: A study was conducted to assess the prevalence of dental caries and its relationship to oral hygiene habits in 440 pre-schoolers living in Erbil, northern Iraq. An oral hygiene questionnaire was distributed among the families. Dental examination of the children was performed to calculate the dependent factor of decayed, missing and filled teeth due to caries (dmf). All data was analyzed by means of the SPSS Microsoft statistical system using descriptive tables to identify the relationship between the dependent and independent variable dmf indexes. In order to find the significances, a Chi-square test, a Fisher’s exact test and a likelihood ratio test were used at level of p
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 81-85; doi:10.20473/j.djmkg.v52.i2.p81-85
Abstract:Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p>0.05) and none existed between serum and egg yolk (p>0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 105-109; doi:10.20473/j.djmkg.v52.i2.p105-109
Abstract:Background: Inflammation of the pericorona due to lower third molar impaction (LTMI) is often diagnosed as pericoronitis. Expression of cyclooxigenase-2 (COX-2) and caspase-1 may be induced by lipopolysacharide (LPS) and cause pyroptosis with minimal inflammation. When LPS activates toll-like receptor (TLR-4), NOD-like receptors containing domain pyrin 3 (NLRP3) inflammasome will activate the release of pro-caspase-1 to caspase-1, followed by the secretion of interleukin (IL)-1β. IL-1β and IL-23 which induces CD4+ Tcells (Th17) to produce IL-17 as a pro-inflammation cytokine. Purpose: This study aimed to identify the respective roles of COX2, caspase-1 and IL-17 in pericoronitis inflammation of the pericorona due to LTMI. Methods: Frozen section samples were produced through LTMI pericorona tissue biopsy using material provided by the Dental and Oral Clinic at Muwardi Hospital, Surakarta. The paraffin block produced was subsequently cut using a clean microtome with the resulting thin slices being placed on an object glass coated with polylysine. A diagnosis of pericoronitis was subsequently made by a pathologist. Immunohistochemical staining for COX-2, caspase-1 and IL-17 was carried out by indirect tyramide signal amplification (TSA) method. Photos were obtained by means of 100X, 200X, 400X and 1000X objective lensed microscopes to qualitatively assess the above mentioned protein expressions. T-Test was conducted in order to establish the difference in expression between the control group and pericoronitis due to LTMI. Results: The presence of a brownish yellow color indicated the expression of COX-2, caspase-1 and IL-17 in pericorona epithelial cells which visible expression categorized as moderate (30-70%). The mean expression of COX-2, caspase-1 and IL-17 was categorized as mild and there was no significant difference between the expression of the three proteins. Conclusion: COX-2, caspase-1 and IL-17 play an important role in the phyroptosis signal of LTMI pericoronitis in cases of low inflammation.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 86-89; doi:10.20473/j.djmkg.v52.i2.p86-89
Abstract:Background: Caries constitute an infectious disease that result from the interaction of bacteria with the host and the oral environment. Streptococcus mutans (S. mutans) represents the main bacterium that causes caries. The ability of S. mutans to form biofilms in the oral cavity is influenced by daily nutrient intake. This study of bacterial biofilm proteins can be used in the manufacture of kits for the detection of infectious diseases such as caries in the oral cavity. A biomarker is required for the manufacture of the detection kit. Consequently, research must first be conducted to determine the molecular weight and density of S. mutans biofilm proteins induced by several different daily nutrients, namely; 5% glucose, 5% lactose, soy protein and 5% iron. Purpose: This study aimed to analyse the density of S. mutans biofilm protein induced by 5% glucose, 5% lactose, soy protein, and 5% iron. Methods: The density of the S. mutans biofilm protein bands induced were measured using EZ Imager Gel DocTM software. Results: A band of biofilm protein (61.7 kDa) was obtained from S. mutans induced by 5% glucose, four bands of biofilm protein (180 kDa; 153,9 kDa; 43,9 kDa; 37,5 kDa) from 5% lactose induction and seven bands of biofilm protein (157,9 kDa; 86,6 kDa; 66,5 kDa; 50,1 kDa; 37,9 kDa; 32,3 kDa; 29,4 kDa) from soy protein induction. In contrast, S. mutans induced by 5% iron did not show any protein bands. The proteins that result from each inducer are of differing densities. Conclusion: The protein bands from each inducer are of different densities which can be used in the further test to make a biomarker for dental caries detection kits.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 61-65; doi:10.20473/j.djmkg.v52.i2.p61-65
Abstract:Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD. Results: A significant difference existed between the number of osteoblasts in the test groups. The highest number of osteoblasts observed was consistently that in the treatment group. Conclusion: The application of 20% snail mucin gel was effective in enhancing the number of osteoblasts in rats suffering from periodontitis.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 100-104; doi:10.20473/j.djmkg.v52.i2.p100-104
Abstract:Background: Tooth eruption, the movement of teeth toward the oral cavity clinically marked by the emergence of the cusp or incisal edge, is very important in determining the chronological age of a child. Unfortunately, tooth eruption in 6-12-year olds on the island of Madura has yet to be investigated. Purpose: This study aimed to analyze differences in permanent tooth eruption between boys and girls aged 6 to 12 years old on Madura. Methods: This study employed an observational analytic design in combination with a cross-sectional approach. The samples used in this study were selected by means of simple random sampling technique. Post-selection informed consent of the child subjects was obtained with their chronological age being assessed and determined prior to tooth eruption. The normality of the data was subsequently analyzed by application of a one sample non-parametric Kolmogorov Smirnov test. Thereafter, repeated Anova tests were conducted to determine differences in the permanent tooth eruption of the subjects. Results: Based on the normality test results, the significance value of the permanent maxillary teeth in the male subjects was 0.993, while that of their permanent mandibular teeth was 0.695. In contrast, the significance value of the permanent maxillary teeth in the female subjects was 0.970, while that of their permanent mandibular teeth was 0.918. According to the results of the repeated measure ANOVA test, differences existed in the eruption of the permanent maxillary and mandibular teeth between the males and females with a significance value (ρ) of 0.020. The mean value of permanent mandibular tooth eruption in the females was 56.59 ± SD 33.403, while that of their permanent maxillary tooth eruption was 50.77 ± SD 34.201. The mean value of the permanent mandibular tooth eruption in the males was 55.31 ± 33.024, while that of their permanent maxillary tooth eruption was 48.77 ± SD 34.201. Conclusion: On Madura, the permanent teeth of chronological 6-12-year old females, particularly their permanent mandibular canine teeth, erupt earlier than those of their male counterparts.
Dental Journal (Majalah Kedokteran Gigi), Volume 52, pp 76-80; doi:10.20473/j.djmkg.v52.i2.p76-80
Abstract:Background: As reported in several studies, prolonged or uncontrolled cases of diabetes mellitus (DM) may induce a more extreme inflammatory response. Mucosal lesions can be observed in patients who present this systemic condition. Purpose: The purpose of this study was to elaborate the management of herpes labialis, oral thrush and angular cheilitis as oral manifestations of diabetes. Case: A 49-year-old male complained of having experienced painful lip ulceration for the preceding seven days. The anamnesis of the patient revealed that, prior to lip ulceration, he had experienced high fever and malaise in addition to frequent urination at night and a tingling sensation in the toes and fingertips on waking. Case management: The patient was referred to undergo blood count, fasting blood glucose and oral glucose tolerance tests. The blood test result confirmed the patient to be suffering from DM and anemia. The immunocompromised condition of the patient prompted the occurrence of herpes labialis, oral thrush and angular cheilitis. Conclusion: Multidisciplinary treatment for herpes labialis resulting in oral candidiasis in DM patients is indispensable. Concurrent infection with HSV and candidiasis necessitates a more prolonged healing process. Consequently, it is a essential to treat the hyperglycemia which induces an immunocompromised state in diabetic patients.