Jurnal Sumberdaya Hayati

Journal Information
EISSN : 2477-037X
Published by: Journal of Consumer Sciences (10.29244)
Total articles ≅ 69
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Tila Mardhatillah, Dorly, Nina Ratna Djuita
Published: 7 June 2022
Jurnal Sumberdaya Hayati, Volume 8, pp 27-33; https://doi.org/10.29244/jsdh.8.1.27-33

Abstract:
Sweet star fruit (Averrhoa carambola L.) is one of Indonesia’s most popular fruit plants. Anatomically, the characters of the local sweet star fruit of Mekarsari Fruit Garden are not widely known, so this study aims to observe the anatomical structure of the leaves. The materials used were star fruit leaves of varieties Demak Jingga, Demak Kapur, Demak Kunir, Welahan, and Wulan. The leaves of star fruit varieties were made microscopic preparations in paradermal incisions using the whole mount method and transverse incisions using the paraffin method. The observation result of the paradermal incision of sweet star fruit leaves showed that the type of epidermal cell wall is straight and shallow grooved. The leaf stomata of five sweet star fruit varieties were parasitic. The calculation of the index value, size and density of stomata varied between varieties. The highest stomata density and index were found in the Welahan variety. The results of the transverse incision of sweet starfruit leaves showed that the five local sweet star fruit varieties have mesophyll tissue of the dorsiventral type. Wulan variety has leaves and mesophyll tissue which tends to be thicker than other varieties.
Fitri Nur`aeni, Diah Ratnadewi
Published: 2 June 2022
Jurnal Sumberdaya Hayati, Volume 8, pp 14-19; https://doi.org/10.29244/jsdh.8.1.14-19

Abstract:
Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine. When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Muhamad Azwar Syah
Published: 2 June 2022
Jurnal Sumberdaya Hayati, Volume 8, pp 20-26; https://doi.org/10.29244/jsdh.8.1.20-26

Abstract:
Lipolytic bacteria attract great attention to various biotechnology industries because of their enzymatic potential. This study aims to isolate and identify lipolytic bacteria from cashew nutshell waste using the 16S rRNA gene as a molecular marker. Lipolytic bacteria were isolated using serial dilutions and inoculated on lipolytic media. A total of 3 isolates of lipolytic bacteria were obtained from cashew nutshell waste based on screening in LA Rhodamine B. The partial sequence of 16S rRNA gene from LB15 amplified using a pair of primers 63F and 1387R having a size of 1238 bp, while BL6 and BK6 were 1283 bp, respectively. Based on genetic distance analysis and phylogenetic reconstruction, we proposed that LB15 be identified as Burkholderia sp. with 99.92% similarity. In addition, because the 16S rRNA gene sequence similarity of BL6 was 99.87% with Paraburkholderia kururiensis strain 979, BL6 was classified as Paraburkholderia kururiensis. Then, isolate BK6 was identified as Ralstonia sp. with a similarity of 99.53%. The similarity value can be used as a reference in determining the identity of bacteria. A bacterium can be categorized as the same species if it has a similarity value of more than 99%.
Henta Ria Anisa, Tri Atmowidi, Windra Priawandiputra, Sih Kahono
Published: 23 May 2022
Jurnal Sumberdaya Hayati, Volume 8, pp 8-13; https://doi.org/10.29244/jsdh.8.1.8-13

Abstract:
Pummelo (Citrus maxima) is a species that has a high economic value and several cultivars are found in Indonesia. These plants interact with many species of insects, either as predators, parasitoids, or pollinators. This study aimed to measure the diversity of insects associated with pummelo and its environmental factors. Observation of insect diversity used scan sampling method in thirty plants on sunny days. Results showed that a totally of 1,388 individual insects belonging to 17 species in 11 families and 5 orders were found, i.e., Hemiptera, Orthoptera, Coleoptera, Hymenoptera, and Lepidoptera. The dominant insect species found were Camponotus sp. (Hymenoptera: Formicidae), Tetragonula laeviceps (Hymenoptera: Apidae), and Chrysolina sp. (Coleoptera: Chrysomelidae). The number of individual insects was high in the morning (07.00-08.00), while the number of species was high in the noon.
Inama Inama, Nugroho Ponco Santoso, Reza Raihandhany, Firman Heru Kurniawan
Published: 23 May 2022
Jurnal Sumberdaya Hayati, Volume 8, pp 1-7; https://doi.org/10.29244/jsdh.8.1.1-7

Abstract:
Orchid is a plant species with a very high level of diversity, consisting of 28,000 species including 763 genera. As one of the mega-biodiversity countries, Indonesia has at least 5000 types of orchids scattered in various places. This study aims to inventory the types of Terrestrial Orchids found in KHDTK Sawala-Mandapa, Majalengka Regency, West Java Province. This qualitative research uses the roaming method with the scope of the Sawala-Mandapa area and by recording the type and coordinates of each Terrestrial Orchid encountered. The primary data taken was recorded using a note sheet to record the type and GPS to record the coordinates of the point. The results obtained are that there are at least four types of Terrestrial Orchids found, including; Geodorum densiflorum (Lam.) Schltr; Nervilia concolor (Blume) Schltr; Nervila campestris (J.J. SM) Schltr; and Perystilus djampangensis J. J. Sm. At the species level, soil orchids in both areas tend to have a clustered pattern with the size of each group at each point being 2-126 individuals.
Slamet Arif Susanto, Dianti Mayadika Putri, Ilma Rahmawati, Made Ari Sanjaya, Sulistijorini
Published: 24 December 2021
Jurnal Sumberdaya Hayati, Volume 7, pp 62-70; https://doi.org/10.29244/jsdh.7.2.62-70

Abstract:
Water availability in Mount Ciremai National Parks (MCNP) is influenced by sustainability of the vegetation. Therefore, tree regeneration around the water springs area needs to be investigated to maintain the sustainability of water springs ecosystem in the future. The purpose of this study was to analyzed and identify natural seedlings of tree regeneration around the water springs area Seda Block MCNP. The collections of vegetation and abiotic data was carried out on 9 sample plots measuring 3 m × 3 m which is purposively placed around water springs. Result show that vegetation in water springs dominate by seedlings Ficus sp., Ficus hemsleyana, and Trevesia sundaica. Shannon-Weiner diversity index in this location was moderate, but evenness and dominance index classified as high and low, respectively. Overall, seedling of trees dominated by orthodox seed-type and shade tolerant plant. The vegetation in this study dominated by plants categorized least concern (LC) based on IUCN red list. Microclimatic of study site affects 58.89% on abundance and distribution of tree seedlings. Therefore, the springs are in the Seda Block MCNP needs to be conserved and maintain its natural vegetation. Keywords: Mount Ciremai National Park, eco-hydrology, canonical correspondent analysis, conservation
Thauhidayatul Hidayah, Anggraini Barlian
Published: 24 December 2021
Jurnal Sumberdaya Hayati, Volume 7, pp 71-77; https://doi.org/10.29244/jsdh.7.2.71-77

Abstract:
Leea angulata is a commonly plant used by the Sasak people as a medicine to heal the wounds, but the effects of L. angulata on wound healing process has not been studied. The aim of this research are to determine the effects of L. angulata bark extracts on wound healing of mouse skin and its influence on the localization of FGF2. 1% of L. angulata bark extracts (P1%) on day 7 can accelerate wound closure amounted at 94.66%, Deep and Superficial Contraction Index of 0.96 and 0.1, epidermis Index and remodeling index of 1.55 and 0.81. L. angulata bark extracts stimulates the synthesis of FGF2 early (3rd day ) compared to the postive control group (5th day). FGF2 in P1% group wa found in epithelial cells in the hair follicles, fibroblasts cell in granulation tissue and epithelial cells in the epidermis. In the positive control group FGF2 was found in in epithelial cell of hair follicle, while in the negative control group, FGF2 undetectable. FGF2 in P1% group detected earlier than the positive control, showing the process of proliferation in P1% accelerated. The results confirm the L. angulata bark extracts accelerate the wound healing in proliferation phase and influence localization of FGF2 in the wound area. Keywords : Leea angulata, wound healing, FGF2, immunohistochemistry
Dela Putri Amalia, Nabila Dhiya Ulhaq, Kania Dewi Rafa, Faradiba
Published: 24 December 2021
Jurnal Sumberdaya Hayati, Volume 7, pp 57-61; https://doi.org/10.29244/jsdh.7.2.57-61

Abstract:
Physical activity is defined as any bodily movement produced by skeletal muscles. Several factors can influence a person's physical activity levels, one of them is work from home (WFH) policy caused by Covid-19. This policy could reduce a person’s physical activity. To maintain health during the Covid-19 pandemic, most people access the internet to find health-related information. However, there is no information about the physical activity level and the relationship between physical activity and health-related internet usage during the Covid-19 pandemic in Indonesian society. This study asked 83 participants from 32 cities in Indonesia to fill the questionnaire related to their activity using the International Physical Activity Questionnaire (IPAQ) and the purpose of internet usage. Most of the respondents had moderate activity levels. Females were more active than males during the Covid-19 pandemic. A higher intensity of working from home negatively correlated with a physical activity score, however, it was not statistically significant. People who used the internet to search for a healthy diet and healthy life information were more physically active than those who did not. Thus, we assumed that people who used the internet to search for a healthy diet and healthy life information have high awareness about their health. Keywords: Physical activity, IPAQ, MET, Internet usage, Indonesia
M. Ubaidilah Hasan Hasan, La Ode Fitradiansyah Fitradiansyah, Fera Susanti Susanti,
Published: 24 December 2021
Jurnal Sumberdaya Hayati, Volume 7, pp 41-48; https://doi.org/10.29244/jsdh.7.2.41-48

Abstract:
Weaver ants Oecophylla smaragdina are social insects that recognize nestmates using pheromone. This study analyzes the feeding behavior and nestmate recognition of weaver ants in the urban area of three different islands (Sumatra: Baturaja; Java: Banyuwangi; Papua: Sorong). The free ants were placed in the arena composed of chicken meat, sugar, bananas as the baits. Ten trapped ants were also located in the arena to explore the nestmate recognition between the free and the trapped ants. Five types of behaviors, i.e., approach, stalking, communication, competition, and agonistic, were recorded in 6 days. Food preferences were indicated by the number of conduct towards the feeding sites, and nestmate recognition was indicated by approaching the trapped nestmate. The results showed that the percentage of the approach behavior of free ants was the highest in the three islands but had no significant difference between Sumatra and Java and between Java and Sorong (p-value >0.05). However, the study showed significant differences for the other four behaviors observed. As predicted, the percentage of chicken meat foraged by the ants was the highest due to the carnivorous behavior. The free ants showed the nestmate recognition to the trapped ants in all behavior, except competition.
Rizkia Adhatirana, Nina Ratna Djuita, Sulistijorini Sulistijorini, Taufikurrahman Nasution
Published: 24 December 2021
Jurnal Sumberdaya Hayati, Volume 7, pp 49-56; https://doi.org/10.29244/jsdh.7.2.49-56

Abstract:
Epiphytic ferns can be found in host trees from the Angiosperm and Gymnosperm groups. Epiphytic ferns in Angiosperm plants host have been widely studied, but there is little known for Gymnosperm plants host. The aim of this study was to identify the species of epiphytic ferns in the Gymnosperm plants host at Cibodas Botanical Garden and to analyze the diversity of epiphytic ferns based on microclimate conditions and the surface texture of Gymnosperms plants host. Epiphytic ferns diversity data was obtained using purposive random sampling method. Factors that influence the occupancy of ferns are analyzed using Principal Component Analysis. Epiphytic ferns in Gymnosperm host at Cibodas Botanical Garden were identified as 18 species including 7 family. The most dominant species of epiphytic fern is Davallia denticulata (59.45%). Diversity of epiphytic fern on Gymnosperm at Cibodas Botanical Garden is moderate (H’ = 1.81).
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