Fifteen strains of bacteria were successfully isolated from agriculture soil samples contains pesticides and from soil moisture around them. The isolates were capable of utilizing chlorpyrifos (Cp) and malathion as the sole source of carbon, phosphorus and energy. High yielding isolate KSCM-08 was identified based on16SrRNA sequencing. Genomic DNA was successfully extracted from bacterial isolate KSCM-08, and the total DNA content was determined to be 734±23 µg/gm. The absorbance ratio of extracted DNA at A260/280 has been found to be 1.96±0.2. 1381 nucleotide sequence of the PCR amplified 16s rRNA gene from bacterial isolate KSCM-08. The 16s rRNA gene sequence comprises 344 bp of adenine (A), 280 bp of thymine (T), 430 bp of guanine (G), and 327 bp of cytosine (C). The G+C percentage was determined to be 54.8%. The BLAST report demonstrates the similar sequences with the KSCM-08 16s rRNA sequence, along with their percentage of identity. The BLASTn analysis of the 1381 bp length 16s rRNA gene sequence of KSCM-08 against the GenBank database revealed that KSCM-08 exhibits similarity with Bacillus species. The evolutionary divergence of bacterial isolate KSCM-08 with its relative members was determined. A satisfactory result was established by the use of the 16S rRNA gene as a marker to evaluate the phylogenetic relationship and the bacteria was confirmed as Bacillus safensis.

Pharmacovigilance plays an vital role in modern technology which helps in monitoring and assessing the high-quality of Drugs, detection and stopping of any unfavorable outcomes of drugs. Pharmacovigilance starts with case processing: this involve safety data collection and coding, case management reporting and submission. In case of clinical trial, it is the investigator or in case of post-marketing trial, it is either the physician or the prescriber or the patient himself who reports the adverse event or any drug-related problem. Pharmacovigilance case processing helps to get clear information regarding the drug and outcomes by using various software technologies. Arugs database, cemflow, vigibase and ICSR are few software used nowadays. Case processing helps to monitor and track all serious adverse events, and medically significant ADRs and other medical related product information followed by timely processing and reporting of such information according with company and regulatory reporting.

The novel coronavirus has become rapidly widespread, resulting in an epidemic throughout China followed by a pandemic, an increasing number of cases in various countries throughout the world. During the Post COVID-19 period, the quality of life of several people has been impacted to varying extents. The physical health of the patients has shown a significant decline after getting recovered from COVID-19. We have done a community survey and have assessed the physical health of 400 subjects and have observed that there is a limitation in the physical activities of the subjects.

Diabetic Mellitus is a group of metabolic disorders characterized by hyperglycaemia, which occurs due to defects in insulin secretion, insulin action, or both. Diabetics distress is an emotional distress response characterized by extreme apprehension, discomfort, or dejection due to a perceived inability to cope with the challenges and demands of living with diabetes. The goals of clinical pharmacist is to management of about medication adherence, to evaluate the quality of life, identify depression levels among diabetic patients, to identify anxiety levels in diabetic patients, to evaluate diabetic distress and its effects among diabetics. Diabetic distress is the major problem associated with diabetics which is making patients losing their self-confidence and positive attitude towards life. Due to diabetic distress, depression and anxiety significant percentage of patients work exhibited poor quality of life. Proper counselling, continuous interaction, effective therapy by the clinical pharmacists could improve patients quality of life, diabetes causative and medication adherence.

Background:  The First Case Of A Novel Coronavirus (COVID-19) Infection Was Detected In Wuhan, Fever And Respiratory Symptoms Have Been Frequently Reported In Patients Infected With This Virus. Aim: It Was Aimed To Detect The Most Commonly Occurred Clinical Symptoms Of COVID19 In Hospitals And Community Settings. Method: Patients Presenting To Southern Hospitals And Community Settings For COVID-19 Infection Were Included In The Study. Assessment Of Demographics And Background Information Of Covid-19 Infection Is Done And Next The Patients Were Questioned For 22 Symptoms And The Results Were Compared. Listing Out The Symptoms And Symptoms Status And Functionality Status Prior to The Collection Of All Data For Statistical Analysis To Represent The Results. Results: A Total Of 403 Patients With COVID-19 Positive Status Were Included In The Study. The Most Common Symptoms In The COVID-19 Positive Group Were: Cough (81.9%), Shortness Of Breath (78.7%), Sneezing (77.4%), Fever (91.4%), Headache (89.6%), Loss Of Taste (69.2%), And Muscle Pain (80.1%). Conclusion: Our Study Showed That Taste And Cough Were Important Markers In COVID-19 Infection.

Chronic kidney disease is a growing epidemic worldwide. Nearly two-thirds of CKD patients have diabetes mellitus, hypertension, and both comorbid conditions. Several disease management programs are developed and they implement with help of physicians, and highly trained clinical pharmacists to improve disease management and improve clinical outcomes. The goals of clinical pharmacist in the management of CKD patients by focusing on Four key areas: To obtain information about medication non-adherence, To assess the patient complications for dialysis, to assess the prevalence of chronic kidney disease patients, To evaluate the clinical pharmacist’s role in improving patient medication adherence, To identify the correlation between CKD and other comorbidities, To observe the drug interactions in prescribed drugs. Lack of patient knowledge on CKD disease conditions, and limited reimbursement, lack of primary care by physicians and health care professionals are all barriers that must be overcome with help of the clinical pharmacist role in the management of CKD.

Quantum dots also known as zero-dimensional Nano materials, which have very distinctive Properties Such as Electro chemiluminescent, Fluorescent, Photoluminescent. because of their unique properties quantum dots have a revolutionary role in Biomedical research. Quantum dots Like Zinc oxide quantum dots, Graphene Quantum dots, Glutathione conjugated carbon quantum dots (GSH-CQDs) and L-cysteine capped ZnS:Mn quantum dots have many applications in diagnosis and treatment. They can easily cross blood brain barrier hence they be used as drug carriers further more because of their capabilities they gather widespread attention in the biomedical research.

Background: ADRs are frequent in patients of MDR-TB on DOTs-Plus drug regimen. Current study was carried out in tertiary care hospital. Recognition of types and frequency of adverse drug reactions in Intensive and continuation phase of MDR- TB Patients. Methods: It was a prospective observational study conducted in Damien Foundation Urban Leprosy & TB Centre, Nellore. All the MDR-TB patients admitted at the directly observed treatment, short course plus (DOTS plus) Center were enrolled and were monitored for ADRs. The causality and severity of the reactions were determined using Naranjo algorithm. Results: A total of 200 tuberculosis patients of MDR-TB on DOTS therapy were enrolled for the study. Out of 200 patients, 10 were dropouts, 10 defaulted so 190 patients assessed for ADRs, 129 patients developed 109 (56.48%) adverse drug reactions. The higher numbers of ADRs were observed in age group 31- 40yrs followed by 21-30yrs which were more common in men. Majority of adverse drug reactions were Gastrointestinal (GI) problems 48 (37.20%), followed by Ototoxicity 7 (5.42%) and Psychiatric Manifestations 3 (2.321 %) and skin problems 3 (2.32%). On evaluation of the causality of ADRs, majority were found to be Possible (57.95%). The severity assessment showed that most of the patients ADRs were of moderate level (42.04%). Conclusions: ADRs are major factor limiting completion of drug therapy under RNTCP and occurrence of drug resistance which requires attention of all health care professionals.

An infectious disease is caused by a parasite (Virus, Bacteria, and Protozoa etc). The infecting organism or pathogen interferes with the normal functioning of the host and can lead to chronic wounds, gangrene and even death. So, providing knowledge regarding the common infectious diseases is very much essential criteria to the common peoples. The study method was proceeded to assess the knowledge, attitudes and practices of the patient regarding the infectious diseases by the self administered knowledge, attitudes and practices (KAP) questionnaire (base line readings) and then included the handover of pre evaluated patient information leaflet as a counselling aid to the study participants. Analysed through Paired t test. The follow up study results were found significant (*P<0.05) when compared to baseline results. The scores obtained for self prepared patient information leaflet used is as in between the 60-70. So, the patient information leaflet (PIL) is considered as ‘standard’ as per the Flesh Reading Ease Formula. Among all Flesh Reading Ease values patient information leaflet for Dengue fever has shown highest value that is 67.40 and lowest value is obtained for the patient information leaflet of Malaria that is 60.05. Our study concluded that the prepared information leaf let for few infectious diseases prevalence and incidence of anticoagulant induced internal bleeding was high. So, risk management plays important role for the better outcome of the therapy.

In the present research work Zileuton tablets were formulated by employing compression coating technology. Initially the core tablets were prepared by using various concentrations of super disintegrates, the formulated core tablets were coated with the polymers by using compression coating technology. All the core and compress coated tablet formulations were subjected to various physical and chemical evaluation tests for core and compress coated tablets. The thickness, hardness and weight variation shown by all the tablet formulations were found within the official pharmacopoeial limits. In vitro release of Zileuton of core tablet formulations ZF5 showed faster drug release after 15 min. Faster drug release can be correlated with the high disintegration and friability observed in this study.