Advanced Biology

Journal Information
ISSN / EISSN : 2701-0198 / 2701-0198
Current Publisher: Wiley (10.1002)
Former Publisher:
Total articles ≅ 91
Current Coverage
SCIE
Filter:

Latest articles in this journal

Published: 24 May 2021
by Wiley
Advanced Biology, Volume 5; doi:10.1002/adbi.202100776

Tim Blomeier, Patrick Fischbach, Leonie‐Alexa Koch, Jennifer Andres, Miguel Miñambres, Hannes Michael Beyer, Matias Daniel Zurbriggen
Published: 24 May 2021
by Wiley
Advanced Biology, Volume 5; doi:10.1002/adbi.202170051

Abstract:
In article number 2000307, Tim Blomeier and co-workers describe the Blue Light-Operated CRISPR/Cas13b-mediated mRNA Knockdown. “Lockdown” introduces optical control of RNA levels utilizing a blue light-dependent switch to induce expression of CRISPR/Cas13b, which mediates sequence-specific knockdown of exo- and endogenous mRNA. Further, they combined the tool with other optogenetic switches for the efficient downregulation of target proteins in mammalian cells.
Published: 24 May 2021
by Wiley
Advanced Biology, Volume 5; doi:10.1002/adbi.202170052

Lian He, Peng Tan, Yun Huang, Yubin Zhou
Published: 24 May 2021
by Wiley
Advanced Biology, Volume 5; doi:10.1002/adbi.202170053

Abstract:
In article number 2000541, Yubin Zhou, Yun Huang, and co-workers developed innovative optogenetic tools to control antibody-antigen interactions, named Sunbody and Moonbody after the Chinese mythological figures Hou Yi the Sun-shooter and Chang'e the Lady in the Moon, respectively. These photoswitchable modular antibody-like scaffolds contain three allosterically sensitive complementarity-determining regions (symbolized by three rabbits on the moon and three flares on the sun surface), which enable optical control of protein degradation, protein delocalization, immunogenic cell death, transcriptional programming, and CRISPR-aided base editing.
Carlos Honrado, Sara J Adair, John H Moore, Armita Salahi, Todd W Bauer, Nathan S Swami
Published: 20 May 2021
The publisher has not yet granted permission to display this abstract.
Zhi Lin, Jiao Liu, Rui Kang, ,
Published: 20 May 2021
by Wiley
Advanced Biology; doi:10.1002/adbi.202100396

The publisher has not yet granted permission to display this abstract.
Carlos Honrado, Sara J. Adair, John H. Moore, Armita Salahi, Todd W. Bauer,
Published: 20 May 2021
by Wiley
Advanced Biology; doi:10.1002/adbi.202100438

The publisher has not yet granted permission to display this abstract.
Naimeh Rafatian, Katrina Vizely, Hadel Al Asafen, Anastasia Korolj,
Published: 19 May 2021
by Wiley
Advanced Biology; doi:10.1002/adbi.202000190

The publisher has not yet granted permission to display this abstract.
Puja Jain, Akihiro Nishiguchi, Georg Linz, Matthias Wessling, Andreas Ludwig, Rolf Rossaint, Martin Möller,
Published: 13 May 2021
by Wiley
Advanced Biology; doi:10.1002/adbi.202000427

Abstract:
Alveolar‐capillary basement membrane (BM) is ultra‐thin (<2 µm) extracellular matrix that maintains integral epithelial‐endothelial cell layers. In vitro reconstructions of alveolar‐capillary barrier supported on synthetic scaffolds closely resembling the fibrous and ultra‐thin natural BM are essential in mimicking the lung pathophysiology. Although BM topology and dimensions are well known to significantly influence cellular behavior, conventionally used BM mimics fail to recreate this natural niche. To overcome this, electrospun ultra‐thin 2 µm poly(caprolactone) (PCL) nanofibrous mesh is used to establish an alveolar‐capillary barrier model of lung endothelial/epithelial cells. Transepithelial electrical resistance (TEER) and permeability studies reveal integral tight junctions and improved mass transport through the highly porous PCL meshes compared to conventional dense membranes with etched pores. The chemotaxis of neutrophils is shown across the barrier in presence of inflammatory response that is naturally impeded in confined regions. Conventional requirement of 3 µm or larger pore size can lead to barrier disruption due to epithelial/endothelial cell invasion. Despite high porosity, the interconnected BM mimic prevents barrier disruption and allows neutrophil transmigration, thereby demonstrating the physiological relevance of the thin nanofibrous meshes. It is envisioned that these bipolar cultured barriers would contribute to an organ‐level in vitro model for pathological disease, environmental pollutants, and nanotoxicology.
Shaohua Ma, Haoran Zhao, Edgar A Galan
Published: 13 May 2021
The publisher has not yet granted permission to display this abstract.
Back to Top Top