EUREKA: Life Sciences

Journal Information
ISSN / EISSN : 2504-5687 / 2504-5695
Current Publisher: OU Scientific Route (10.21303)
Total articles ≅ 238
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Olena Koshel, Fedor Pertsevoy, Sergei Sabadash, Mykola Mashkin, Valentyna Mohutova, Vadym Volokh
Published: 5 October 2020
EUREKA: Life Sciences; doi:10.21303/2504-5695.2020.001437

Abstract:
The research is devoted to the determination of the properties of the components of the developed milk-containing thermostable fillings and the establishment of the dependence of the effective viscosity on the temperature of the model system. In the work, studies are carried out for a model system containing xanthan gum; a model system containing tara gum; model system containing gelatin; a model system containing xanthan gum and tara gum; a model system containing xanthan gum, tara gum and gelatin; a model system containing xanthan gum, tara gum and sugar; model system containing xanthan gum, tara gum, skimmed milk powder; a model system containing xanthan gum, tara gum and maltodextrin. In this work, the dependence of the effective viscosity on the temperature of the model system is established. To achieve the aim, the following objectives are set: - determination of the dependence of the effective viscosity on the temperature of model systems with xanthan gum, tara gum, gelatin, sugar, skimmed milk powder and maltodextrin with different concentrations of these components; - establishment of the temperature range in which a sharp increase in the effective viscosity of the investigated model systems begins. An increase in the temperature of the system prevents gelation due to an increase in the intensity of Brownian motion and a decrease through it in the duration of the existence of bonds that arise between macromolecules. At the same time, a decrease in temperature promotes gelation, since this increases the number of contacts between macromolecules, which contributes to an increase in the strength of the so-called spatial network. In the article, the dependences of the effective viscosity on the temperature of model systems with xanthan gum, tara gum, gelatin, sugar, skimmed milk powder and maltodextrin with different concentrations of these components are obtained. Based on the obtained dependences, the established temperature ranges in which a sharp increase in the effective viscosity of the studied model systems begins
Alyona Polishchuk, Michael Zavelevich, Daniil Gluzman
Published: 30 September 2020
EUREKA: Life Sciences pp 29-33; doi:10.21303/2504-5695.2020.001429

Abstract:
The cytological and immunocytochemical features of the lymphocytes with villous morphology in peripheral blood and bone marrow in some B-lymphoproliferative disorders were studied. The diagnosis of hairy cell leukemia, a hairy cell leukemia variant, splenic marginal zone lymphoma and splenic diffuse red pulp small B-cell lymphoma was ascertained in accordance with the new revision of the WHO classification (2016). The neoplastic cells of hairy cell leukemia were determined by the presence of high tartrate resistant acid phosphatase (TRAP) activity. Cell surface expression of CD19, CD20 and CD21 antigens was detected. Also, the expression of CD25, CD103 and CD200, and in some cases cyclin D1, was found out. CD5, CD10 and CD23 were not detected. The immunophenotype of cells in splenic marginal zone lymphoma with villous processes also corresponded to the mature B cells. The expression of CD19, CD20 and CD21 was observed in all cases, CD11c – in 50% of patients, CD25 or CD5 – in 10% of patients. In 80% of patients, the pathologic cells did not show TRAP activity. In the bone marrow and peripheral blood cells of patients with diffuse red pulp lymphoma, TRAP activity was not detected. An immunophenotype in the hairy cell leukemia variant was different from those of classic HCL (CD19+CD20+CD22+CD103+CD11c+CD5–CD10–CD23–). Characterized immunophenotypical markers, which have differential diagnostic values in several forms of lymphoid tumors of B cell origin, will be important for the choice of treatment methods and prognosis
Vitaliy Fedorenko, Yana Medvid
Published: 30 September 2020
EUREKA: Life Sciences pp 21-28; doi:10.21303/2504-5695.2020.001431

Abstract:
The research was conducted in 2017–2019 years in the Institute of Plant Protection of The National Academy of Agrarian Sciences of Ukraine in the Laboratory of entomology and resistance of agricultural crops against pests (Kyiv, Ukraine). The objective of the research is investigation of particular biology features of Harmonia axyridis Pall. in comparison with the main species of coccinellids in typical agrocoenosis. The research methods are the following: laboratory method that means laboratory maintenance of coccinellids; mathematical-statistical method that means statistical processing of the obtained results. The Coccinellidae family (Latreille, 1807) has an important economic value in the human business activities. Most species are predators of aphids, scale insects, thrips, spider mites, leafhoppers, whiteflies, eggs and larvae of other small arthropods. Coccinellids are marked by their high gluttony, polyphagous, fertility. Beetles and larvae of ladybirds play a significant role in the quantity control of pests of agricultural, forest and ornamental plants. Since 2009 researchers in Ukraine have devoted great attention to identification and distribution of invasive species of Asian lady beetle, or Multicolored Asian lady beetle, Harlequin ladybird, Halloween ladybeetle (Harmonia axyridis Pallas, 1773). H. axyridis Pall. is characterized by its wide ecological plasticity, trophic specialization, greater gluttony, fertility and number of generations leading to the gradual displacement of local species of coccinellids. The comparative results of researches of some biology features of H. axyridis Pall. and the most common species of ladybirds are presented. The survival of coccinellids imago during the wintering, in the active period in the absence of feed, their gluttony for different species of aphids is examined. Features of oviposition and duration of various stages of coccinellids development are presented. In the laboratory the Asian lady beetle was marked among other species by the number of its laid eggs, oviposition period, duration of various stages of its development, survival and gluttony
Gregoriy Deynichenko, Tamara Lystopad, Anna Novik, Line Chernushenko, Andrii Farisieiev, Yuliiа Matsuk, Tatiana Kolisnychenko
Published: 30 September 2020
EUREKA: Life Sciences pp 43-50; doi:10.21303/2504-5695.2020.001435

Abstract:
One of the acute problems of our time is iodine deficiency in human nutrition, which is typical for both countries with economies in transition and economically developed ones. Today, about a third of the world's population live in conditions of natural iodine deficiency. The most common solution to iodine deficiency is salt iodization, but this can lead to excessive iodine intake. In addition, it is much healthier to eat organic iodine, which is found in large quantities in brown algae. It is the development of new types of food products using brown algae as a biologically active additive that is a new promising direction for solving the problem of insufficient iodine consumption. Taking into account the relevance of solving the problem of iodine deficiency and the constantly growing demand for fruit and berry sauces, the technology of berry sauces based on blueberries, cranberries, dogwood, sea buckthorn and viburnum with the addition of brown algae has been developed. However, the mass introduction into production and consumption of the developed sauces will make a significant contribution to solving the global problem of insufficient consumption of organic iodine, requires research to establish their compliance with the typical quality and safety indicators for these products. The physicochemical indicators of the developed berry sauces were investigated, confirming the high quality in terms of the content of soluble solids – 25.0 ... 28.5%, the mass fraction of titratable acids – 1.9%, and indicate the complete absence of mineral, vegetable and foreign substances in the product. It was found that the developed sauces with iodine-containing algal additive have high microbiological quality indicators when stored for up to 14 days, even without adding preservatives. The complete safety of the developed berry sauces was established in terms of the content of toxic elements, as well as the absence of genetically modified organisms in the finished product
Liudmyla Kryvoplias-Volodina, Oleksandr Gavva, Anastasiia Derenivska, Oleksandr Volodin
Published: 30 September 2020
EUREKA: Life Sciences pp 34-42; doi:10.21303/2504-5695.2020.001434

Abstract:
The complex of technical means for optimization synthesis of assembling of a packing machine of separate functional modules has been developed. The method of synthesis of a packing machine, based on criterial assessment of separate functional modules (FM), combined by two main assessment groups, has been offered. FM may be selected and calculated by the program of consumption, based on the overall equipment effectiveness (OEE) criterion. An example of synthesis, based on the offered method, takes into account variants of choice of ready functional modules, based on the hierarchic structure of a module of roll packing material supply. The method takes into account the systemic approach to analysis of equipment constructions for packing fine-piece and piece food products in a consumption package. The synthesis of FM assembling as conceptual models, abstract ones, reflecting the construction structure and connections between separate elements – functional devices (FD) – has been offered. The optimal assembling of the functional device in the structure of the functional module of roll packing material supply has been determined. As a result of solving this problem, a FM1 prototype has been created. At conducting the comparative analysis with the existent equipment, the automatic functional device has been modeled. The use of the OEE criterion with joint properties that reflects the generalized assessment of a packing machine or functional module with a maximin (minimax) criterion by the compromise principle has been substantiated. The analysis is grounded on the idea of optimality of each module or device of the machine for packing food products at adding each next functional module to its composition. The program of assessment calculation of the package equipment with the complex assessment criterion OEE for different assembling of FMi machines for packing piece and fine-piece products has been developed. The FM of roll film material supply with using a microprocessor managing device that maintains a sinusoidal law of movement of a stretching roll of the packing machine has been developed. Optimal characteristics of the technical system have been determined. Results, obtained at processing experimental data, confirm adequacy of the offered method for assessing assembling solutions
Volodymyr Khomych, Olha Fedorenko
Published: 30 September 2020
EUREKA: Life Sciences pp 16-20; doi:10.21303/2504-5695.2020.001402

Abstract:
Rabbits are an important livestock animal species, which are used for their meat and fur. Nowadays they are also becoming more popular as pets. Furthermore, rabbits are commonly used in research, inter alia in immunological studies and for studying pathogenesis of human and animal diseases. The lymphoid tissue is abundant in the rabbit intestine and a lot of it is concentrated in Peyer's patches, the majority of which is located in the jejunum. Understanding of the rabbit Peyer's patches functions is essential for the prevention and treatment of their diseases. In order to enhance it, accurate knowledge of its lymphocyte population composition is needed. At present, the cellular composition of the rabbit gut-associated lymphoid tissue remains insufficiently studied. Therefore, the aim of our study was to establish the content and localization of lymphoid populations in the domestic rabbit jejunal Peyer’s patches. We performed immunohistochemical studies using monoclonal antibodies CD3, CD10 and CD20 (DAKO, Denmark) to identify the distribution of lymphoid populations in jejunal Peyer's patches of a mature domestic rabbit. The results, obtained in our study, complement the existing data and determine the main lymphoid populations in the rabbit jejunal Peyer's patches. The presented data are fundamental for further studies of the rabbit gut-associated lymphoid tissue. We found that lymphoid nodules of jejunal Peyer's patches contains a predominant CD20+ cell population (B cells). It also contains many CD10+ cells (precursors of T cells and B cells), which are mostly concentrated in the germinal centers of lymphoid nodules. In the diffuse form of the lymphoid tissue the vast majority of cells are CD3+ cells (T cells)
Kyrylo Samoichuk, Alexandr Kovalyov, Vadym Oleksiienko, Nadiia Palianychka, Dmytro Dmytrevskyi, Vitalii Chervonyi, Dmytro Horielkov, Inna Zolotukhina, Alina Slashcheva
Published: 30 September 2020
EUREKA: Life Sciences pp 51-58; doi:10.21303/2504-5695.2020.001436

Abstract:
The competitiveness improvement of milk products is directly connected with a problem of decreasing energy consumption of the process of milk emulsion dispersion. At creating promising types of energy effective dispersers, a necessary condition is to elaborate a correct methodology for studying them that takes into account the specificity of the process of fat milk microemulsion dispersion. Based on the critical analysis of research methods for homogenizers of different types, there have been determined main directions of their improvement, taken into account in the elaborated research method for a promising jet-slot type milk homogenizer. The method of theoretical studies, including a choice and analysis of stable and changeable factors of the homogenization process in a jet-slot type homogenizer and optimization criteria, has been elaborated. The interconnection of technological, constructive, hydraulic parameters with a dispersion quality has been demonstrated. The influence of physical-mechanical properties of milk as a research object was taken into account. An optimal milk temperature was chosen for the studies. Factor variation limits have been substantiated. The constructive scheme of a chamber for the experimental homogenizer has been developed. At processing the experimental research methodology, the main stage was to develop an experimental set for studying the influence of changeable factors of the dispersion process on a homogenization quality. Dispersion quality estimation methods have been analyzed and the method of microphotography has been chosen with further computed analysis of obtained data. The elaborated methodology was used at studying the influence of cream fat and ring slot width on a dispersion quality in a jet-slot type homogenizer. The obtained data allow to determine rational parameters of cream fat – 40–50 % and slot width – 0.1 mm. The analysis of dispersed characteristics of the milk emulsion allows to make a conclusion about the high homogenization quality, comparing with processing in a valve homogenizer
Published: 24 September 2020
EUREKA: Life Sciences; doi:10.21303/2504-5695

Anastasiya Khokhryakova
Published: 23 September 2020
EUREKA: Life Sciences pp 3-15; doi:10.21303/2504-5695.2020.001404

Abstract:
The purpose of the research – 1) establishment of classification and diagnostic characteristics of soils under different functional using of the city territory; 2) parameterization of the structure, composition and properties of anthropogenically transformed and anthropogenically created soils; 3) development of a classification scheme, taking into account its use in cadastral and economic grouping. General scientific (systematic, analysis, synthesis, generalization, statistics) and traditional (soil-genetic, profile-morphological, laboratory-analytical and cartographic) research methods were used to study the genetic characteristics of soils of Odessa and their classification. The ecological-profile-genetic classification of soils of urban areas, which combines profile-genetic and factor-ecological approaches, is proposed. Within the city there are natural, anthropogenically transformed and anthropogenically created soils. Soils during cadastral and economic grouping are united into two groups of transformed and technological soils of human settlements and other territories with anthropogenically transformed soil cover, are distinguished. According to cadastral and economic grouping, within Odessa the area of transformed soils is 11,915.6 ha, technological soils is 1922.5 ha. The proposed scheme of soil classification of urban areas includes 2 supertype levels, 3 types and 13 subtypes of soils, which are divided according to their genetic profile and combined according to the characteristics of natural and anthropogenic factors of soil formation. For urban cadastre, organization, planning and implementation of land management, economic activity, a scheme of supplementing the list of agricultural soils of Ukraine with cadastral and economic grouping of soils of urban areas was proposed. The grouping of soils includes two associations: 1) transformed and 2) technological (artificially created) soils - a total of 14 cadastral and economic groups
Bogdan Putyatin, Ekaterina Kunitsia, Natalia Sytnik
Published: 31 July 2020
EUREKA: Life Sciences, Volume 4, pp 15-20; doi:10.21303/2504-5695.2020.001374

Abstract:
Development of a new generation of functional antiage cosmetics is a most important area of cosmetic industry development. An integral part of such products are biologically active substances (BAS) with essential oils, being among the sources of which. Each essential oil is characterized by a well balanced unique composition of biologically active substances. Development of new cosmetic products consists of correct selection of the required composition of components with required properties and selection of a suitable package, preserving product properties throughout its shelf life. During production of cosmetic products in aerosol package a number of issues are solved, unlike with antiage-products in form of creams in customary tubes. The degree of oxidation-reduction reactions, having negative impact on the properties of essential oils, is substantially reduced. The concentration providing for the optimum degree of evacuation and excess pressure in the packaging are essential issues. The aim of this research is to determine the degree of evacuation of the contents, excess pressure, and mass fraction of a propellant in the product test samples to find the samples of aerosol package with optimum parameters. A number of tests to determine the degree of evacuation of the contents from aerosol package, excess pressure and a mass fraction of a propellant have been performed. The greatest degree of evacuation of products was observed with mass fraction of propellant of 13.79%. Based on the results of the tests for excess pressure in aerosol packaging, it has been found, that with mass fraction of a propellant of 13.79% excess pressure is 0.4 MPa, which was taken as an optimum value. The test showed that the mass of a propellant of 16 g evacuates the product from aerosol package to the maximum extent and does not exceed the acceptable limits for excess pressure (0.2–0.6 MPa)
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