Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies

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ISSN / EISSN : 2413-5550 / 2518-1327
Former Publisher: Oles Honchar Dnipropetrovsk National University (10.15421)
Total articles ≅ 1,438
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V. M. Nedashkivskyi, B. D. Mishchenko
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 46-49; doi:10.32718/nvlvet-a9409

Abstract:
On the development and productivity of bjolin queens, they infuse the methods of vivennya, dew vivification, weather conditions, and the appearance of food - bee bread and honey, or a square file and nectar. On a practical basis, the preparation of the large family of creepers with food, additional supplies of small private substitutes, one of such substitutes is glucose-fructose syrup. The purpose of this work was to study the effect of carbohydrate supplementation with glucose-fructose syrup in foster families on the number and weight of reared queens in the context of supportive honey. In the statistic, it has been infused into the adolescent bjolin family with glucose-fructose syrup on the efficiency of the growth of bjolin queen bees. The pre-session was carried out at the base of the PP “Volodymyr” with. Shershny Tivrivsky district of the Vinnytsia region. In the past year, in the year of the bdzholin family, glucose-fructose syrup was revealed, which shortens the result of the reception of larvae per vykhovannya to preserve in the previous groups by 12.5 % more portions of the control group in bjolins. The water hour revealed a decrease in vibrating mothers by 10.5 %, depending on the control group, in which case the indicator of the increase in changes was made. In the results of the last few days, it was established that the introduction of glucose-fructose syrup to the last group of glucose-fructose syrup to the last group was increased, and the increase in the intensity of the growth of the bjolin queens became freely average, to a maximum of 20.3 % for the group. Thus, the obtained data indicate the effectiveness of the effect of glucose-fructose syrup during feeding in foster families on the number and weight of reared queens.
O. P. Rudenko, N. A. Lytvyn, B. V. Gutyj
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 81-85; doi:10.32718/nvlvet-a9415

Abstract:
There are a number of springs on the territory of Lviv, water from which is used by the city residents. It should be noted that natural resources cannot be systematically used by the population for drinking purposes, as they are not sources of centralized water supply and, accordingly, do not have service personnel. No sanitary protection zones have been established for such sources, within which economic activities are prohibited. In this regard, the quality of water from such natural sources has unstable indicators, which depend on many factors of man-made and natural factors (sanitary-chemical and sanitary-microbiological indicators can sometimes fluctuate within one week), which makes it unfit for human consumption and can lead to acute intestinal infections of bacterial and viral etiology, a number of infectious diseases. The article considers the current ecological, microbiological state of natural sources of the city of Lviv. The necessity, and hence the relevance of the analysis and detailed microbiological assessment of water quality of springs in the city of Lviv, namely: springs № 1 in the park "Zalizna Voda" (Lviv, Ternopilska Street); springs № 2 in the park "Zalizna Voda" (Lviv, Myshuhy Street); springs № 3 in Stryiskyi Park (Lviv, Stryiska Street). A sociological survey of city residents on the use and consumption of water from the studied sources was conducted. The results of the analysis were compared with the list of maximum concentration limits of harmful substances in water bodies in accordance with the order № 400 from 12.05.2010 on approval of State sanitary norms and rules “Hygienic requirements for drinking water intended for human consumption” (DSanPiN 2.2.4-171-10).
A. V. Hunchak, S. M. Medvid, O. M. Stefanyshyn, Ya. M. Sirko, S. I. Koretchuk
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 50-55; doi:10.32718/nvlvet-a9410

Abstract:
The paper presents a fragment of a systemic study on the efficiency of replacing inorganic salts of microelements in guaranteed mineral premixes used in quail feed with a complex supplement of said elements in nanocitrate form produced by Nanomaterials and Nanotechnologies LLC (Kyiv). The study is, in particular, focused on the levels of microelements (Mn, Zn, Cu, Fe, Co, І) in quails and their egg yolks depending on the form and concentration of those microelements in the poultry diet. The tests have been conducted on 14-day old Pharaoh quails divided into three groups. Raising conditions – cages. Test duration – 2 months. Quails of all groups were fed complete feed with a balanced content of nutrients and biologically active compounds. Control group birds were given mineral premix containing inorganic salts of (g/t of feed): Mn – 50, Zn ‒ 50, Cu ‒ 2.5, Fe ‒10, Co ‒1 and І ‒ 0.7. Test group quails were fed a mineral complex of aqua citrates of the same elements with a concentration of (calculated for each element) 1/10 (D1) and 1/20 (D2) or 10 and 5 % of their content in a standard mineral premix (SP). It is established that the form and dosage of microelements introduced into the diet affect their levels in quails and their egg yolks. Nanocitrates of bioelements have a relatively high cumulative potential in poultry compared with the control group (an inorganic form of microelements). The best results are achieved with citrate elements being fed in a concentration of 10% of their regular content in a standard mineral premix. It is proven that the use of optimal concentration of aqua citrates of microelements in poultry diet promotes increased levels of manganese, iron, zinc, copper and cobalt (Р < 0.05‒0.001) in the liver and hip muscles as well as iron and copper levels (Р < 0.01‒0.001) in breast muscles of quails; increased body weight by 15.7 % during the testing period; an enhanced biological value of the eggs (increased calcium (Р < 0.05), iodine (Р < 0.05), zinc (Р < 0.01) levels) compared to analogs in the control group.
V. M. Volovich
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 20-24; doi:10.32718/nvlvet-a9404

Abstract:
The results of studies on the effect of synthetic tryptophan supplements to goose feed during the reproductive period on the incubation qualities of eggs, the level of total protein, albumin and α-, β- and γ-globulins in the blood of embryos and newborn goslings are presented. The study was conducted on four groups of geese-analogues of the Obroshyn gray breed group during the 3-month period (January ‒ March). The control group did not receive synthetic tryptophan supplements. The level of this amino acid in this group of geese in accordance with current regulations in Ukraine was 0.16 g per 100 g of feed. To the diet of birds 2, 3 and 4 experimental groups were additionally administered, respectively, 0.04; 0.09 and 0.14 g of synthetic tryptophan per 100 g of feed. As a result of research it was found that increasing the level of tryptophan from 0.16 g to 0.30 g per 100 g of feed due to its synthetic analogue in the diet of geese during egg-laying significantly improves the incubation quality of eggs and has a positive effect on protein levels. daily embryos and 5-day-old goslings. It is shown that the most pronounced effect on improving the quality of hatching eggs and optimizing the effect on the level of protein in the blood of embryos and goslings reveals the content of tryptophan in feed during intensive laying of geese in the amount of 0.25 g per 100 g of feed. Studies have shown that the quality of hatching eggs and the content of total protein, albumin and α-, β- and γ-globulins in the blood of embryos and newborn goslings has a pronounced positive effect on the level of tryptophan in the diet of geese during intensive laying. In particular, increasing the amount of this essential amino acid in goose feed during the reproductive period from 0.16 to 0.30 g due to its synthetic analogue improves such incubation qualities of eggs as: egg-laying, weight, length, width, egg shape index, strength and thickness eggshells, and increases in the serum of 25-day-old embryos and 5-day-old goslings contents of total protein, albumin and α-, β- and γ-globulins (P < 0.05). It is shown that the most pronounced positive effect on the optimization of protein ingredients in the serum of both embryos and goslings was found for the content of tryptophan in the feed of geese of the parent flock in the amount of 0.25 g per 100 g of feed. The results show that increasing the level of such essential amino acids as tryptophan in goose feed during intensive egg-laying from 0.16 to 0.25 g per 100 g of feed activates the synthesis of egg components in the ovary of birds, improves their quality and has a stimulating effect on the biosynthesis in hepatocytes of embryos and newborn goslings of total protein, its albumin, α-, β-globulin fractions and immune γ-globulins.
, S. V. Sliusarenko, A. O. Sliusarenko, R. А. Petryshak, I. P. Golodyuk, O. S. Naumyuk, O. І. Petryshak, O. V. Kuliaba
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 3-8; doi:10.32718/nvlvet-a9401

Abstract:
According to modern classification of trace elements, which is based on their biological significance for living organisms, selenium is classified as a group of vital or biogenic elements. As a biotic element, it has unique physico-chemical and biochemical properties and, with adequate intake into the body of farm animals and poultry has a positive effect on a number of physiological processes. The discovery of biological properties of selenium became the basis for its use first in the prevention and treatment of many diseases associated with a deficiency of this trace element, and later – as a stimulator of growth and development of young animals, as well as in order to increase egg production, poultry safety, improve the incubation characteristics of eggs and several other productive qualities. Scientists who have studied the effects of selenium on poultry have paid relatively little attention to meat quality. The effect of additives of different selenium doses in compound feed on the chemical composition, energy and biological value of Ukrainian white breed ducklings’ meat was studied in the scientific and economic experiment. Four groups of ducklings with 100 heads in each groups were formed to conduct the scientific and economic experiment. The duration of the experiment was 56 days and corresponded to the period of raising ducklings for meat. The ducklings of the first control group did not receive selenium supplementation. Selenium was additionally introduced into compound feed for poultry of the experimental groups in the following amount, mg/kg: the second group – 0.2; the third – 0.4 and the fourth – 0.6. It was established that the introduction of selenium into compound feed in the studied dose did not significantly affect to the quality of ducklings’ meat, although it had a positive effect on some indicators that characterize its chemical composition, nutritional and biological value. Among the experimental groups, ducklings of the third and fourth groups, which were injected with selenium into compound feed at the rate of 0.4 and 0.6 mg/kg, stood out in terms of meat quality.
K. O. Skoryk, S. V. Girin, S. Е. Kokish, І. І. Tatsyuk, O. V. Dobrovolskyi
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 31-35; doi:10.32718/nvlvet-a9406

Abstract:
The presented work presents the results of the effectiveness of the bioregulatory approach in stimulating sports horses. The aim of the study was to find a way to safely restore the energy balance and prevent the occurrence of inflammatory processes in the musculoskeletal system during significant physical exertion. Sport horses spend a lot of energy not only in competition, but also during daily training. Such loads require a high supply of energy. Often the body uses its last reserves to compensate for its energy needs, which leads to exhaustion and the appearance of various diseases. First of all, diseases occur in the musculoskeletal system, because it is the limbs that bear the greatest load during training. As you know, various synthetic doping is introduced to keep animals in a sporty tone. Their action is not long-lasting, but it has many side effects for the animal's body and leads to disruption of the functioning of organs and systems. Today, there is an alternative to such stimulation methods thanks to a bioregulatory approach in the treatment of animals using preparations containing exclusively natural ingredients. To confirm the effectiveness of the use of bioregulatory drugs, clinical and laboratory studies of animal blood (general, biochemical, cytochemical) were carried out. The main changes in laboratory parameters were obtained by cytochemical analysis of such enzymes as GPDH, SDH. These enzymes are among the main indicators of the intensity of energy metabolism in the horse's body. The quantitative composition of basophils and eosinophils has also changed, which indicates a decrease in the level of the inflammatory process in the body of experimental animals. The results obtained prove the effectiveness of the investigated drugs and their direct influence on the energy balance of the “horse-athlete”. Thanks to the natural composition of the components of the preparations used, side effects on the body can be avoided and used without restrictions throughout the horse's sports career.
A. S. Kramarenko, S. I. Lugovoy
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 25-30; doi:10.32718/nvlvet-a9405

Abstract:
The main aim of this paper was to estimate the piglet stillbirth incidence distribution in the Large White (LW) sows. The population used for the present study is from a pig farm managed by the Limited Liability Company (LLC) “Tavriys’ki svyni” located in Skadovsky district (Kherson region, Ukraine). The experimental materials used for this study consisted of 100 inds. of productive parent sows of the Large White breed. The estimation of reproductive performance was conducted for each animal included in this study. The total number of piglets born (TNB), number of stillborn piglets (NSB) and frequency of stillborn piglets (FSB) per litter were monitored in the first eight parities in the period of eleven years (2007–2017). The 800 farrowings averaged 11.3 piglets per litter. Of the 9037 piglets born, 7895 were live born and 1142 were stillborn. Stillborn pigs were observed in 63.3 ± 1.7 % of the litters and the pig stillbirth incidence was 12.6 ± 0.4 %. The percentage of pigs born dead in total pigs born, obtained in this study is within the range of rates (5 to 15 %) reported for commercial pig farms in other countries. The frequencies of litters with 1, 2, 3, 4 and 5 or more stillborn piglets were 27.1, 14.8, 10.6, 5.8 and 5.0 %, respectively. The frequency of litters with at least one stillborn piglet varied from 44 % (2nd parity) to 76 % (6th parity). Significant factor in the analysis for frequency of litters with at least one stillborn piglet was parity number in sow (Chi-squared test: χ2 = 51.35; df = 7; P < 0.001). This frequency increased as parity number in sow increased (Pearson correlation coefficient: r = 0.929; P < 0.01). We noted that the piglet stillbirth incidence in the LW sow’s litter took place already during the first farrowing for almost half of the sows (46%). In general, this distribution is well approximated by the exponential model equation (y = 73.567*exp(-0.551x); R2 = 97.09 %). The LW sows with stillborn piglets in four out of eight farrowings were the most reported. The value of the entropy of the traits NSB and FSB varied significantly among sows of different parities (Chi-squared test: in both cases P < 0.001). At the same time, a significant increase in the entropy estimates for both traits from the 1st parity to the 8th parity was noted (Spearman's rank correlation: P < 0.001…0.002).
I. I. Kushnir,
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 9-15; doi:10.32718/nvlvet-a9402

Abstract:
Data on the study of the effect of a consortium of lactic acid bacteria on the morphological parameters of blood and the state of the intestinal microflora of white rats using a composition of probiotic strains of L. lactis, Lb. plantarum and E. durans SB18 in a ratio of 50:40:10. Thus, when determining the morphological parameters of the blood of rats at 14 days of use found a positive effect of probiotic strains of lactic acid bacteria on the hematopoietic function of laboratory animals. In particular, a significant increase in hemoglobin concentration, erythrocyte count, leukocyte count and hematocrit value was found by 14.9, 14.0, 22.5 % (P < 0.05) and 5.7 %, respectively, compared with the control group. In addition, the use of the drug for 14 days caused an increase in the average concentration of hemoglobin in the erythrocyte (MCНS) by 9.8 % (P < 0.05) and a tendency to decrease the average hemoglobin in the erythrocyte (MCН) and the average erythrocyte volume (MCV) by 0.45 and 9.0 %, respectively, compared with the control group. Both central and peripheral organs of the immune system were also significantly affected. In particular, the relative weights of the thymus and spleen increased by 21.1 (P < 0.05 ) and 9.8 % (P < 0.05), respectively. When determining the leukogram of peripheral blood of rats, it was found that the use of the study drug in animals of the experimental group caused a tendency to increase the number of neutrophils, lymphocytes and monocytes by 6.0, 0.5 and 19.8 %, respectively, with a slight decrease in eosinophils. The use of a composition of a consortium of lactic acid bacteria stimulated an increase in lacto- and bifidobacteria content of the large intestine of white rats. In particular, a significant increase in the number of bifidobacteria and lactobacilli was found by 4.3 and 5.2 % (P < 0.001), respectively, relative to the animals of the control group. In addition, 14-day application of the studied composition of probiotic strains contributed to a probable reduction in the number of opportunistic and putrefactive microflora. The number of Escherichia coli, Staphylococcus aureus and fungi of the genus Candida significantly decreased relative to control by 4.7 (P < 0.01), 2.6 (P < 0.05) and 13.3 % (P < 0.001), respectively, all this indicated the recovery of the body of laboratory animals. The positive effect of lactic acid bacteria of the formed composition persists until the 21st day of the experiment.
V. V. Senechyn, E. O. Yakimova
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 56-60; doi:10.32718/nvlvet-a9411

Abstract:
We have studied the possibility of increasing the production of young carp by applying a set of intensification measures and the introduction of innovative production methods, when growing it in artificial reservoirs. Works that has been carried out works provided with technology of cultivation of young fishes as one of the first stages of production of marketable products in aquaculture, secures the selection of qualitative and viable breeding material, providing it with appropriate environmental conditions for normal growth and development, search of optimum and balanced compound feeds which will supplement natural fodder base of ponds, and carrying out of complex measures concerning intensification of fish farming taking into account natural-climatic and geographical-zonal features of a separate economy. The main purpose of the work was to improve the technology of growing young fish in the carp farm of Ltd "Mykolaivska RMS" when using in the feeding of this year's granular feed. Generally accepted hydrochemical, hydrobiological and fish farming methods were used in order to achieve this goal. The technological features of growing carp fish planting material at low planting densities were studied in the ponds of the experimental fishery. The effect of land reclamation, fertilization of ponds to improve the natural feed base, feeding fish with granular feed with a well-balanced content of nutrients and biologically active substances, with constant control of temperature and hydrochemical regimes of ponds on morphometric parameters expediency of carrying out such measures in fisheries. Our research results indicate that the natural fodder basis of ponds of the experimental carp farm Ltd "Mykolaivska RMS" does not fully meet the regulatory conditions for growing young carp and carrying out the above set of intensification measures in combination with feeding young carp granular concentrated organic feed environmentally friendly and economically feasible.
O. O. Borshch
Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, Volume 23, pp 36-41; doi:10.32718/nvlvet-a9407

Abstract:
Recently, an important element in improving the genetic traits of dairy cattle on commercial farms is the use of interbreeding (crossbreeding). The use of crossbreeding makes it possible to use a variety of additive genetic levels between breeds to grow individuals with higher economic potential. The aim of this study was to analyze the development of udder parameters in crossbred first-borns compared to purebred counterparts and to study the indicators of milk production during milking. The research was conducted on the first-born Ukrainian black-spotted dairy breed and first-generation crossbreeds obtained by crossing with the Brown Swiss breed and on the Ukrainian red-spotted dairy breed and first-generation crossbreeds obtained by crossing Ukrainian red-spotted dairy breed with Montbeliarde breed. Two groups of purebred and local first-borns were formed in both farms. Both farms use the same type of year-round feeding of cows with complete feed mixtures. The study was performed on first-born cows during weaning (2–3 months of lactation). It was found that local first-borns obtained by crossing red-spotted and Montbeliarde breeds outnumbered purebred red-spotted analogues in terms of measurements and udder indices. The opposite situation was observed in local first-borns obtained as result of crossing black-spotted and Brown Swiss breeds, which were inferior in these characteristics to purebred black-spotted analogues. In purebred black and red-spotted cows, the rates of single and daily milking were higher than in local ones. Also, purebred first-borns had a longer duration of single milking, indicators of average and maximum intensity of milk production and milk yield in the first 3 minutes. Prospects for further research are to study the productivity for completed lactation, the quality of milk and the duration of the service period.
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