ASPIRATOR - Journal of Vector-borne Disease Studies

Journal Information
ISSN / EISSN : 2085-4102 / 2338-7343
Current Publisher: Badan Penelitian dan Pengembangan Kesehatan (10.22435)
Total articles ≅ 81
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Arda Dinata, Endang Puji Astuti, Suwarno Hadisusanto
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 105-114; doi:10.22435/asp.v12i2.2270

The vector-borne disease remains a health problem in Pandeglang District. Entomological data isimportant in the strategy of controlling the vector-borne disease. This study aimed to determine thedistribution of mosquito larvae based on specific habitat and ecosystem typea. This research is a secondarydata analysis of Rikhus Vektora 2016 in Pandeglang, Banten Province. This type of observational researchwith cross-sectional study design. The purposive sampling technique is used based on geographical andecosystem stratification. We found 12 types of environments of the six types of ecosystems (HDP, HJP, NHDP,NHJP, PDP, and PJP) that had larvae: forest (secondary, homogeneous, and coastal); lagoon; brackish waterswamp; bamboo clumps; rice fields; plantations; and residential areas. The most ecosystem types werelarvae in HJP (160 larvae) and the least larvae in NHDP (9 larvae). Species of larvae are 16 types: rice fields(Cx. vishui, Cx. tritaeniorhynchus, An. barbirostris); small hole in the ground (Anopheles sp., Culex sp.);coconut shell (Ae. albopictus, Ar. malayi, Ar. kuchingensis, Malaya sp, Culex sp.); armpit taro leaves andbanana leaves (Ae. albopictus, Malaya genurostris); freshwater swamp (Cx. vishnui, Cx. gelidus) andbrackish water (Anopheles sp.); riverside (Anopheles sp., Cx. quinquefasciatus); ditch (Culex sp.); pool (Cx.quinquefasciatus); lagoon (Anopheles sp., Culex sp.); bamboo stumps (Ae. albopictus); Limnocharis flavagarden (Culex sp.); and used bottles (Ae. albopictus). The characteristics of larvae habitat: temperature (25-33oC); pH 8-9 (67.3%); light intensity (115-32,000 lux); vegetation (12.7%); algae (3.6%); water istemporary (61.6%), inundated (78.2%) and clear (50,9%).Keywords: Habitat, Ecosystem, Larvae Vector, Pandeglang
Muhammad Nirwan, Upik Kesumawati Hadi, Susi Soviana, Surachmi Setyaningsih, Fadjar Satrija
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 93-104; doi:10.22435/asp.v12i2.2710

Filariasis is still a health problem in Bogor Regency. The discovery of filariasis sufferers hasgrowing during the year since 2004 until now with a total of 117 people. The mass prevention drugadministration program (POPM) has been implemented since 2015. This study aims to identify theepidemiological distribution of clinical filariasis sufferers and an overview of the achievement of filariasiselimination program in Bogor district. The research using descriptive design with a quantitative approach.The data in this study used secondary data from Bogor District Health Office and Bogor Central Bureau ofStatistics. Data were analyzed descriptively and identify differences and relationships between variablesused the chi-square test. The results of the study showed the epidemiological distribution of filariasis inBogor Regency with predominantly female patients (59.8%) and productive age (36-45 years). The resultsfrom chi-square test showed that there was a significant difference between the age groups and theincidence of filariasis from year to year with a P value (0.000) 0.05. The spread of filariasis tends to fluctuate and continues to increase (75%). The results of therelationship test showed that there was no significant relationship between the number of cases and the levelof family welfare with a P value (0.279)> 0.05. The implementation of POPM, both treatment outcomes andtreatment success rates, has exceeded the national target.Keywords: Epidemiology, Filariasis, POPM, Bogor, Indonesia
Zainul Ambiya, Martini Martini, Firda Yanuar Pradani
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 115-122; doi:10.22435/asp.v12i2.1440

Tembalang Village is one of the endemic areas of DHF in the city of Semarang (IR 479,6/100.000population). The discovery of chemical resistance cases in mosquitoes requires another alternative as an effortto control dengue that is environmentally friendly, cheap and effective, namely by using mosquito traps withattractants. This study aims to determine the differences in the types of effective attractant materials to beused in mosquito traps as an effort to control mosquitoes. This type of research is experimental with the Post-Test Only Control Group Design method. The sample of this study was 64 houses with repetitions of 6 times.Testing of attractants was carried out on 3 types of attractants, namely brown sugar yeast, sugar yeast, strawsoaking water and PAM water as a control. Environmental observations were carried out to determine thedensity of mosquitoes in Tembalang Village. The results showed that the type of attractant most favored bymosquitoes was brown sugar yeast (73,37%) and sugar yeast (26,62%). Meanwhile, the mosquito density inTembalang sub-district was low at 1.375 with the most found mosquito population being Culex (55.7%) andthe highest fishing place in the house (59%). Keywords: Tembalang Village, Vector Control, Attractant, Mosquito Density
Asti Tri Pramadani, Upik Kesumawati Hadi, Fadjar Satrija
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 123-136; doi:10.22435/asp.v12i2.3269

Dengue hemorrhagic fever (DHF) is still a problem in some countries, resulting in 390 millioninfections a year in the world. DHF vaccine has not found so the treatment is focused on vector controls.Entomological data describing the bio ecology of vectors in the region can help vector control diseases moreeffectively. The study aims at assessing distribution, characteristic and risk factors in the habitat of Aedes larvain the DHF endemic region of West Ranomeeto subdistrict of Southeast Sulawesi province. This study is ananalytic observational study with a sectional study approach. Data collection was carried out using singlelarva method in 600 houses by purposive sampling. Data analysis uses chi square’s descriptive analysis andlogistics multinomial regression. The result shows larva density in Sindangkasih higher than Jati Bali. Ae.aegypti dominated in Sindangkasih and Ae. albopictus dominated in Jati Bali. Buckets, dispenser tray,refrigerator tray, container made of cements and plastics and container with volume
Lukman Hakim, Endang Puji Astuti, Heni Prasetyowati,
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 73-84; doi:10.22435/asp.v12i2.3140

One House One Jumantik Programme (G1R1J) has been launched by the Indonesian governmentsince 2015. This programme emphasizes the participation of family members as jumantik rumah bymonitoring and controlling larvae in their houses. Family’s coaching in the G1R1J’s programme is carried outby each jumantik coordinator. Tasikmalaya and Cimahi were Dengue endemic areas with high cases in thelast five years. This study aimed to determine the effect of family empowerment by the Jumantik Coordinatorin reducing the density of Aedes spp. larvae, reducing the number of DHF cases and increasing familyparticipation in vector surveillance. The study was located in the Tasikmalaya and Cimahi areas andconducted with an intervention. The interventions included RW-level workshops, coaching, and observationby jumantik coordinator. The sample unit is a family, consist of 400 unit in the intervention area and 200unit in the comparison area. The results showed that there were significant differences in the status ofcommunity participation in eradicating mosquito nests (PSN). The presence of dengue patients and thepresence of Aedes spp mosquito larvae were different between before and after the intervention both inTasikmalaya and Cimahi. In addition, there are significant differences in the status of communityparticipation in PSN, the presence of dengue cases, the presence of Aedes spp. larvae and theimplementation of vector surveillance by families in the intervention and comparison areas. The resultsconcluded that family coaching interventions and observations by the Jumantik Coordinator, proved to havean effect on community participation in PSN, decreasing dengue cases, increasing larvae free index (ABJ)and vector surveillance implementation by families.Keywords: Society participation, Dengue cases, Jumantik House, One House One Jumantik
Aryo Ginanjar, Laksono Trisnantor, Dwi Handono Sulistyo
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 63-72; doi:10.22435/asp.v12i2.3109

Dengue Hemorrhagic Fever (DHF) is still a public health problem that is difficult to deal with inTasikmalaya City, West Java Province. In 2019, the region experienced very significant cases increase leadingto an Outbreak. Various attempts have been made without optimal results. DHF control program managersexpressed the urgent need for strong policies, but no empirical studies have been carried out. It is importantto analyze evidence as justification for urgency and impetus in the policy agenda. This policy research withnaturalistic qualitative research with Analysis for Policy approach aims to explore evidence on the urgency ofDHF control regional regulations at policy stakeholders’ aspect. The results of this study revealed the evidenceof DHF Control Regional Regulation’s establishment urgency. Those are increasing community endangeringsituation of DHF cases; the urgent need of policies for program managers; and the corroborating evidence, asthe results of stakeholder analysis, in the form of majority stakeholder are the main policy stakeholder whohave the power and strong interest in supporting the policies’ urgency. The urgency level of DHF controlRegional Regulation based on this research has reached an emergency level, so there should be efforts toestablish DHF control Regional Regulation in Tasikmalaya City.Keywords: Stakeholders, Policy Urgencies, Regional Regulations, DHF
Nurminha Nurminha, Tori Rihiantoro, Mara Ipa
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 85-92; doi:10.22435/asp.v12i2.3095

Clinical symptoms of dengue virus (DENV) infection range from asymptomatic mild dengue fever(DF), more severe dengue hemorrhagic fever (DHF) up to dengue shock syndrome. One of the determinantsof dengue infection severity was virus virulence. This study aimed to determine the clinical and virologicalcharacteristics of dengue virus infection patients based on the severity degree. A cross-sectional study wasconducted in RSUD Dr. H. Abdul Moeloek, Lampung Province between July-November 2016 with 56 denguepatients as samples selected using purposive sampling. The serological test was done using a rapiddiagnostic test. Blood samples for DENV serotype identification were examined using reverse-transcriptionpolymerase chain reaction. Classification of DENV infection severity was obtained from the patient’s medicalrecord. The results showed that the most common clinical manifestations were fever, headache, and retroorbitalpain, appearing in all patients from every degree of severity. There were Grade I DHF patients whoexperienced Myalgia (15.6%) and petechiae (22.2%). Laboratory results showed that thrombocytopeniaappeared in every grade, even though 13.3% of grade I patients did not experience it. Secondary infectionwas found in 92.9% of samples, and all DENV serotype can be detected in 39.2%samples: DENV-1 (46.7%),DENV-2 (6.7%), DENV-3 (26.7%), and DENV-4 (20%). This study concluded that the majority of clinicalcharacteristics in DHF patients are in line with the degree of severity, with the bleeding as the dominantmanifestation in patients with grade II-IV. Virological characteristics of DENV-1 are dominant in all patientswith DHF grade I-IV.Keywords: dengue virus, serotype, severity, secondary infection, Bandar Lampung
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12; doi:10.22435/asp.v12i1.3388

Acivrida Mega Charisma, Elis Anita Farida, Farida Anwari
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 11-18; doi:10.22435/asp.v12i1.2366

Dengue is a disease caused by dengue virus infection. Diagnosis of the disease is often difficult to be upheld just by the symptoms. Therefore, laboratory diagnostics is required. The general objective of this study was to detect dengue IgG in serum samples with rapid tests and in urine samples of suspected dengue patients using ELISA. The procedure of this study consisted of three stages. First, collecting urine samples from healthy individuals and collecting blood and urine samples from suspected dengue patients; second, examining dengue IgG in serum samples of suspected dengue patients with rapid tests; and third, examining dengue-specific IgG in urine samples from suspected dengue patients and from healthy individuals with ELISA technique. Data analyzed using experimental analytics to determine the cut off point used to interpret laboratory diagnostic results. The results showed that patients with positive serum dengue IgG test at the same time also showed positive results on the examination of dengue-specific IgG in their urine samples while patients with negative serum at the same time showed negative results on the examination of dengue-specific IgG in urine samples. Dengue-specific IgG examination in urine samples with ELISA technique can be used as an alternative examination in establishing the diagnosis of dengue, in which urine samples are taken from patients with more than 4 days fever. Keywords: Dengue, IgG, Urine, ELISA Abstrak. Dengue merupakan penyakit yang disebabkan virus dengue. Diagnosis penyakit ini sering sulit ditegakkan jika hanya melihat dari gejala. Oleh karena itu, diperlukan pemeriksaan laboratorium untuk penegakkan diagnosis. Tujuan umum penelitian ini adalah untuk mendeteksi IgG dengue dalam sampel serum dengan rapid tes dan pada sampel urine pasien terduga dengue dengan menggunakan metode ELISA. Prosedur penelitian ini terdiri dari tiga tahap. Pertama, pengumpulan sampel urine individu sehat dan pngumpulan sampel darah dan urine pada pasien terduga dengue; kedua, pemeriksaan IgG dengue dalam sampel darah pasien terduga infeksi dengue dengan rapid tes; dan ketiga, pemeriksaan IgG spesifik dengue pada sampel urine pasien terduga dengue dengan teknik ELISA, yang sebelumnya sudah dikelompokkan sesuai dengan hasil pemeriksaan IgG dengue pada sampel serum dengan rapid tes, dan pemeriksaan IgG spesifik dengue sampel urine individu sehat dengan teknik ELISA. Analitik eksperimental digunakan untuk menentukan cut off dari data yang kemudian digunakan untuk menafsirkan hasil diagnostik laboratorium. Hasil penelitian menunjukkan bahwa pada pasien dengan hasil pemeriksaan IgG dengue serum positif menunjukkan hasil positif juga pada pemeriksaan IgG spesifik dengue pada sampel urinnya, sedangkan pada hasil pemeriksaan serum yang negatif menunjukkan hasil negatif pula pada pemeriksaan IgG spesifik dengue di sampel urinnya. Pemeriksaan IgG spesifik dengue pada sampel urine dengan teknik ELISA dapat digunakan sebagai pemeriksaan alternatif dalam penegakan diagnosis dengue, di mana sampel urine diambil dari pasien dengan demam lebih dari 4 hari. Kata Kunci: Dengue, IgG, Urine, ELISA
Muhammad Umar Riandi, Tri Wahono, Mara Ipa, Joni Hendri, Subangkit Subangkit
ASPIRATOR - Journal of Vector-borne Disease Studies, Volume 12, pp 37-44; doi:10.22435/asp.v12i1.2765

Japanese encephalitis is a mosquito-borne zoonotic disease that has pigs as the amplifying host. It is important to study the diversity of mosquito species around pig populations to determine the potential of Japanese encephalitis vectors in the region. This study is a cross-sectional study with a single sampling method for adult mosquitoes around pigpen in Tangerang Regency. The capture of adult mosquitoes is carried out by the outdoor resting mosquitoes collection at 18:00 - 24:00 using aspirators and light traps. Female mosquitoes were identified and subsequently tested RT-PCR for JEV. Catching results obtained 223 mosquitoes from the genus Culex, Armigeres, Aedes, Anopheles, and Mansonia with a total of 10 species. Species diversity in the pigpen area is classified as moderate (H = 1.0875 – 1.292) with Culex vishnui and Culex quinquefasciatus as the most abundant species. RT-PCR test found there’s no mosquito’s samples with positive JE RNA virus. Several species of mosquitoes found around pigpens in Tangerang District have the potential to become JEV vectors, so that control to the mosquito abundance and health of pigs is needed as a preventative measure. Keywords: Diversity, Japanese encephalitis, pig, vector Abstrak. Japanese encephalitis merupakan penyakit zoonosis yang ditularkan oleh nyamuk dan babi sebagai inang penguat. Studi keanekaragaman jenis nyamuk di sekitar populasi babi penting dilakukan untuk mengetahui potensi vektor Japanese encephalitis pada wilayah tersebut. Desain penelitian ini adalah potong lintang dengan metode sampling tunggal terhadap nyamuk dewasa sekitar kandang babi di Kabupaten Tangerang. Penangkapan nyamuk dewasa dilakukan dengan metode koleksi outdoor resting pada malam hari pukul 18.00 – 24.00 menggunakan aspirator dan perangkap cahaya. Nyamuk betina diidentifikasi dan selanjutnya diuji RT-PCR virus JE. Hasil penangkapan didapatkan 700 ekor nyamuk dari genus Culex, Armigeres, Aedes, Anopheles, dan Mansonia dengan total 10 spesies. Keanekaragaman spesies pada seluruh wilayah penelitian tergolong sedang (H=1,0875 – 1,292) dengan Culex vishnui dan Culex quinquefasciatus sebagai spesies paling melimpah. Uji RT-PCR tidak menemukan adanya sampel nyamuk positif RNA virus JE. Beberapa spesies nyamuk yang ditemukan di sekitar kandang babi di Kecamatan Panongan memiliki potensi sebagai vektor JEV sehingga diperlukan pengendalian kepadatan nyamuk dan kesehatan ternak babi terhadap JEV sebagai upaya pencegahan. Kata Kunci: Keanekaragaman, Japanese encephalitis, babi, vektor
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