Journal La Lifesci
ISSN / EISSN : 2721-1304 / 2721-1207
Published by: Newinera Publisher (10.37899)
Total articles ≅ 42
Latest articles in this journal
Journal La Lifesci, Volume 2, pp 1-16; https://doi.org/10.37899/journallalifesci.v2i4.425
The aim of this research was to produce Streptomyces-extracellular chitinase and screen its antifungal activity on a clinically isolated Candida albicans. The Streptomyces were isolated from an agricultural farmland; they were identified and screened for the chitinase production. Effects of time, temperature, pH and nitrogen sources on the chitinase production were determined using standard methods. Ammonium sulphate precipitation was used to partially purify the chitinase. Protein concentrations were determined spectrophotometrically using bovine serum albumin as standard. Agar-well diffusion method was used to evaluate the antifungal activity of the chitinase on C. albicans. The isolated Streptomyces were of three (3) strains, and all the strains are Gram positive, catalase positive, oxidase positive while, Strain A and C are indole positive and only Strain B is citrate positive. The maximum chitinase production was at 72 h, 40°C and when yeast extract was used as the nitrogen source. Ammonium sulphate (80%) precipitation yielded the highest enzyme activity of 39.0U/ml. The maximum enzyme activity was observed at temperature of 40oC, pH 5.5 and 1.0% colloidal chitin (substrate). The partially purified chitinase showed a zone of inhibition of 20.11 ± 1.26 mm against the Candida albicans. This result has no significant difference (P>0.05) when compared with that of the standard drug (Fluconazole) with 21.42 ± 0.08 mm zone of inhibition. These findings suggest that Streptomyces at favourable conditions produce chitinase, and this enzyme can be used as an antifungal agent on Candida albicans and other chitin containing fungi.
Journal La Lifesci, Volume 2, pp 17-24; https://doi.org/10.37899/journallalifesci.v2i4.451
Jackfruit is used as a meat substitute by some consumers but reports on its use are scanty. Jackfruit is becoming popular among meat lovers who want non-soy-based meat alternative. Thus, this study aims to 1) discover a healthy and affordable option to meat and 2) assess if consumers candifferentiate between pulled pork and jackfruit in a sandwich. Three types of sandwiches were prepared (i.e., jackfruit-in-water; pork tenderloin; jackfruit-inbrine). Sensory analysis using 300 untrained panelists was conducted to examine selected quality attributes (flavor, texture, aroma) and identify the meat-based sample. Panelists scored the sandwiches on a scale of 1-3 with 1 being most favored. The mean scores were calculated as follows: pork = 1.54), jackfruit in brine = 2.16), and Jackfruit in water = 2.18. Statistical analysis (2 tailed T-test) found a significant difference (p = 0.003) in flavor between thepork sandwich (score = 1.6) and those of jackfruit in brine (score = 2.17) and water (score = 2.17). There was a significant difference (p = 0.05) in the texture of pork sandwich (score = 1.6) and jackfruit sandwich in brine (score = 2.0) and water (score = 2.3). Most tasters thought the jackfruit was a meat product although the meat product was ultimately favored. Participants did not know that the sandwiches had a meat substitute. Jackfruit could be a desirable meat substitute for consumers who are looking for low-fat meat alternative rich in antioxidants to add to meals.
Journal La Lifesci, Volume 2, pp 39-50; https://doi.org/10.37899/journallalifesci.v2i3.418
This review focuses on the environmental pollutants and contaminants of emerging concern in Africa. There is increasing reports and identification of ecotoxicological impact of contaminants of emerging concern (CECs), such as plastic, pharmaceutical products, modern pesticides, and personal care products, in terrestrial and aquatic environment within Africa. Identification of CECs, including pharmaceuticals, plastic wastes, pesticide residues, fungal toxins, and personal care products, have been documented in African region including in sediments, sludge, treated drinking water, surface water, wastewater, groundwater, land, solid deposits, etc. Some of the emerging pollutants of concern to environment and humans include lindane, heptachlor, endrin, endosulfan, dieldrin, dichlorodiphenyltrichloroethane (DDT), benzaldehyde, aldrin, bisphenol A, phthalates, bisphenol S, etc. There is need for more studies to identify and quantify the existing and more emerging pollutants.
Journal La Lifesci, Volume 2, pp 27-38; https://doi.org/10.37899/journallalifesci.v2i3.416
This research was designed to evaluate the antipyretic activity of K. senegalensis leaf extract with the view of isolating and identifying the active components. The K. senegalensis leaf was extracted using 90% methanol and further fractionated with hexane, ethylacetate, n-butanol and distilled water.The qualitative phytochemical screening was carried out using standard methods.The crude extract and the fractions were screened for antipyretic activity using 15%w/v brewer’s yeast induced pyrexia on Albino rats. The components of the most active fraction were further separated using column and thin layer chromatographic techniques on silica gel. LD50 of the most active fraction was determined using probit analysis. The results of the phytochemical screening revealed the presence of tannins, phenols, steroids and cardiac glycosides in both the crude extract and its fractions. The crude extract at 400 mg/kg b.w. showed the highest antipyretic activity compared to the other doses tested. Hexane fraction showed the highest antipyretic activity among the other fractionated extracts. The LD50 of the hexane fraction was found to be 831.76 mg/kg b.w. The column chromatographic separation of the hexane extract yielded 60 fractions (F1 to F60). After TLC separation, fractions with similar profile were pooled together yielding eleven (11) pooled fractions (PF1 to PF11). Antipyretic activities of the pooled fractions showed that PF8 exhibited the highest activity. These findings suggested that, K. senegalensis leaf has significant antipyretic activity which can be considered for the development of antipyretic agent from natural resources.
Journal La Lifesci, Volume 2, pp 1-7; https://doi.org/10.37899/journallalifesci.v2i3.362
Profit loss is a phenomenon caused by the loss of most of the harvest resulting in operating costs greater than the revenue earned. This phenomenon deserves to be analyzed the risk of losses that will be obtained due to pests and plant diseases. So that in this study an analysis of the risk of Profit loss due to yellow mosaic disease on papaya calina was carried out. This research was conducted in October 2020 in Bengkulu Province on 31 Calina Papaya farmers. The location selection was carried out purposively based on the existence of the Calina papaya garden. Meanwhile, the selection of respondents was carried out using the Snowball sampling method in several districts in Bengkulu Province. Data analysis was carried out to determine the Profit loss. Based on the results of the analysis, it was found that there were differences in the benefits of papaya calina which were attacked by the Yellow Virus Mosaic and those that were not attacked by the Yellow Virus Mosaic. Profit loss for a year is Rp 9,135,203,-. This value is a big value, so farmers must handle this disease better.
Journal La Lifesci, Volume 2, pp 8-14; https://doi.org/10.37899/journallalifesci.v2i3.377
This study discusses profits and break even point of jamnapari goat farming. Goat and sheep husbandry offers an excellent image that may demonstrate how it might promote a wide range of economic activity. Goats are simple to breed, need little initial capital, require little animal feed, and are easily adapted to their surroundings. The goat is a kind of animal that provides many advantages to the society. Goats are an alternate source of revenue for goat farming businesses looking to expand their operations. The Jamnapari Crossbred goat is a kind of indigenous goat that has strong development potential and may help to boost the income of the surrounding area's farmers. Diversification (multi-commodity farming) is an alternative to carrying out business efficiency on relatively fixed land areas while increasing productivity.
Journal La Lifesci, Volume 2, pp 15-26; https://doi.org/10.37899/journallalifesci.v2i3.389
Cassava is an important staple food around the world, especially in Africa. It is cultivated throughout the Ivorian territory with predominance in the South. Due to the high demand, some producers opt for an early harvest, neglecting the impact of the harvest stage on the sensory parameters of cassava root derivatives. The objective of this work was to determine the stage of harvest allowing obtaining products of good sensory quality. The sensory characteristics of the attiéké were evaluated at different stages of harvest (11th, 12th, 13th and 14th months after planting the cuttings) of the cassava roots of the Yacé variety which were used for its production. The attiéké obtained in the twelfth month of harvest was generally much appreciated because it was less acidic, less fibrous and more homogeneous and had a better smell. Thus, cassava harvested in the twelfth month of cultivation makes it possible to obtain attiéké with the best sensory characteristics. Therefore, this stage of maturity of cassava roots is recommended for attiéké producers.
Journal La Lifesci, Volume 2, pp 14-23; https://doi.org/10.37899/journallalifesci.v2i2.374
This article discussed about the conversion of agricultural land into other uses is one of the occurrences that has occurred fairly often in recent years. The method utilized with the purposive sampling , including but not limited to certain criteria that have been established. The informants that participated in this research were ten (10) individuals who were chosen based on a variety of factors. The kind of data that was utilized was qualitative data of a certain kind (descriptive). The result of this research is the factors that influence land use change: I external factors, which include economics and demographics, which cause the conversion of community citrus plantations to oil palm plantations under the pretext of abundant yields and income, in order to meet the daily needs of the community; (ii) internal factors, where farmers are greatly aided by the presence of this type of palm tree, due to its high productivity; and (iii) internal factors, which cause the conversion of community citrus plantations to oil palm plant Economically, the conversion of citrus farms to oil palm plantations has had a positive effect since the revenue from oil palm farming is higher than that from citrus farming.
Journal La Lifesci, Volume 2, pp 31-39; https://doi.org/10.37899/journallalifesci.v2i2.375
The aims of the study is to nutritional content of bromelain enzyme fermented coconut dregs as feed for oreochromis niloticus. The research procedure bromelain enzyme preparation, coconut dregs fermentation process, experimental design. The result of the research is the proximate test that has been carried out on coconut pulp that has been fermented using the bromelain enzyme, it reveals many things. Namely, the water content and ash content of the coconut pulp that has been fermented using the bromelain enzyme is deemed excellent since it displays a value of less than 12 percent . It is different with crude protein which is less than the National Norm on feed since it only displays a value of 6.20 percent when the standard is 20-35 percent . Another with crude fat and crude fiber. Crude fat and crude fiber in coconut dregs that have been fermented with bromelain enzymes have risen and are far from standard. The normal crude fat is only 2-10 percent , whereas the crude fat in coconut pulp after fermentation with bromelain enzymes is more than 20 percent . And the normal crude fiber is only 4-18 percent , this is less than the crude fiber in fermented coconut pulp because it displays more than 29 percent.
Journal La Lifesci, Volume 2, pp 1-7; https://doi.org/10.37899/journallalifesci.v2i2.334
This research aims to identify the process of adopting innovation of calina papaya into Papaya Sauce for women farmer groups to increase the income of women farmer groups. The research was conducted in the village of Rindu Hati with a purposive method. The location selection was due to the existence of a farmer business group that cultivates and manages calina papaya. In this study, the determination of respondents was carried out by census with 32 female subjects who were cultivating and cultivating papaya plants. Data analysis used descriptive methods to assess the characteristics of the data. The results of the research are: First, the knowledge stage is in the medium category for the knowledge function and the high category for the knowing process. Second, persuasion forms a good attitude towards the adoption of innovation in the high category. Third, the getting to know stage is still in the Medium category. Fourth, the Implication stage using innovation is still relatively moderate. Fifth, confirmation of the adoption of papaya sauce innovation is still in the Medium category.