Ukrainian journal of veterinary sciences

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ISSN / EISSN : 2663-967X / 2663-9688
Total articles ≅ 118
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V. A. Gryshchenko, National University of Life and Environmental Sciences of Ukraine, J. Illek, V. A. Tomchuk
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.006

Abstract:
Diseases of the digestive system are extremely common among animals, especially young animals. The purpose of this work was to determine the features of the corrective effectiveness of reparative agents based on phospholipids of various origins based on the results of a comprehensive study of clinical, hematological, immunological parameters and the structural and functional state of immunocompetent organs in CBA mice with experimental gastroenteropathology.As a result of the reproduction of an acute form of hemorrhagic gastroenteritis with diarrheal syndrome in CBA mice, the development of characteristic clinical symptoms was noted. Regardless of the nature of the origin of phospholipids, preparations based on them exhibited a pronounced reparative effect on cell damage. At the same time, the effectiveness of the dietary supplement "FLP-MD" based on phospholipids from milk according to some indicators turned out to be better than the preparation Essentiale-forte based on phospholipids from soybeans. Thus, the peculiarity of the effect of the dietary supplement "FLP-MD" on the organism of sick mice is the acceleration of the restoration of the general clinical state of the animals (on the 8-10th day from the beginning of the introduction of the dietary supplement into the body versus the 10-12th day when using the drug essentiale-forte), restoration of the mass of lymphoid organs (thymus and spleen); an increase in the relative number of T-lymphocytes in the blood by 1.4 times due to the activation of proliferative processes in the organs of the immune system (4.8 times in the spleen, 1.5 times in the thymus).
V. F. Radchikov, D. M. Bogdanovich, A. N. Kot, V. P. Tsai, V. A. Tomchuk, V. A. Gryshchenko, V. I. Karpovskyi, National University of Life and Environmental Sciences of Ukraine
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.003

Abstract:
Calf rearing is critical to a successful dairy or beef cattle breeding. Maximum productivity at the lowest cost can be obtained from healthy calves only. Zootechnical, biochemical and economic research methods were used during the experiments.It has been determined that feeding calves with experimental milk replacer with milk period duration of 65 days allowed to obtain 692 g of the average daily weight gain for the experiment period, that is 2.3% lower than the control indicator. Feeding calves with WMR helps to reduce the cost of diet by 6.0% and the price cost of weight gain by 3.6%.Use of dry skimmed milk replacer and whole milk replacer during the rearing period, with milk period duration of 115 days, contributed to decrease in weight gain by 1.8 and 1.2% compared to the control variant. Feeding calves with DSMR and WMR helped to reduce the cost of diet by 10.3 and 5.8% and the price cost of weight gain by 8.5 and 4.8%.Feeding with DSMR and WMR in diet for young animals over 115 days of age during the post-milk period increases concentration of erythrocytes in blood by 10.0 and 9.8%, hemoglobin - by 5.8 and 3.9%, amount of glucose - by 11.0 and 9.5%. Inclusion of DSMR and WMR in diet for young cattle with milk period duration of 65 and 115 days contributed to increase in the average daily weight gain in the post-milk period by 9.2 and 10.7%, while reducing the cost of feed by 5.8 and 7.6%, price cost of weight gain by 6.1 and 7.7% and increase in additional profit in the amount of 8.35 (3.5 USD) and 10.58 (4.5 USD) rubles per animal during experimental period.In the future, it is necessary to improve the feeding system of young cattle using new developments in this area.
J. Illek, D. Kumprechtová, V. A. Tomchuk, V. A. Gryshchenko, I. V. Kalinin, National University of Life and Environmental Sciences of Ukraine
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.001

Abstract:
The aim of the study was to compare effects of two levels of selenium yeast (SY) and sodium selenite (SS) supplementation on blood, colostrum and milk Se levels, blood chemistry parameters in dairy cows under field conditions. The study included 36 Holstein cows from 5 weeks prepartum to 12 weeks postpartum. The cows were divided into 4 treatments (n = 9): 1. Negative Control (NC) – no Se supplement; 2. Positive Control (PC) – 0.3 ppm Se (from sodium selenite); 3. S1 – 0.3 ppm Se (from selenium yeast – SELSAF®, Lesaffre Feed Additives, France); 4. S2 – 5 ppm Se (SELSAF®, Lesaffre Feed Additives, France). At 5 and 1 weeks prepartum, on the calving day, and 4, 8, 12 weeks postpartum, blood samples were taken. Colostrum and milk were collected on the calving day and 4, 8 and 12 weeks postpartum and analysed for Se content. Initial Se status in all the cows was marginal. Negative Control cows showed suboptimal blood Se levels for the whole period under study. S1 and S2 showed significantly (P < 0.01) higher values than NC and PC cows. Colostrum Se levels were significantly higher in S1 (128.17 ug/L) and S2 (155.58 ug/L) than in NC (94.68 ug/L) and PC (99.3 ug/L) cows.Overall mean milk Se levels in S1 (20.4 ug/L) and S2 (50.0 ug/L) were higher than in NC (10.9 ug/L) and PC (16.6 ug/L). Biochemical indicators of blood did not significantly differ between the treatments and were within the physiological ranges.Se levels in whole blood, colostrum and milk, and GSH-Px activity were significantly increased by selenium yeast supplementation. The response was dose dependent and consistent. Selenium from organic source was more bioavailable than selenium from sodium selenite. Dietary Se at a tolerance dose (5 ppm) had no detrimental effect on the blood chemistry parameters analysed.
M. O. Zhukovsky, V. V. Nedosekov
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.008

Abstract:
The article examines the foundation , stages of formation and development of the economyanimal health . The main scientific schools and their contribution to the development of animal health economics are highlighted .It is established that the economics of animal health is a dynamic and relatively new field of research , currently little explored are economic tools that cover the problems of animal health and the functioning of the veterinary service. Relevant issues that directly engaged and involved , now , a small group of scientists, consultants and Narrow specialists.Currently, new priority areas for the development of animal health economics are the study of the economics of animal husbandry systems and animal health problems due to the impact of these systems, in particular transboundary emergent animal diseases.Undoubtedly, one of the main criteria for sustainable animal husbandry is animal welfare and biosecurity, which are priorities of the One World, One Health concept established by the FAO, OIE and WHO Triumvirate in 2009. It is the consolidation of these criteria and the laws of economics that will ensure the sustainable development of animal husbandry.It is extremely necessary to establish our own scientific school that will take into account the world experience, realities and peculiarities of veterinary medicine and animal husbandry and be implemented in modern science and production of Ukraine.
A. O. Oripov, N. E. Yuldashev
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.007

Abstract:
The modern strategy to control the main, widespread helminthiasis of farm animals provides for a complex of anthelminthiasis measures, including: planned therapeutic and prophylactic deworming, based on data from the study of the epizootology of helminthiasis in each specific climatic-geographical zone; the implementation of measures for the pasture prevention of geohelminthiasis and the fight against the main, intermediate and additional hosts of pathogens of biohelminthiasis, including zoonotic helminthiases (echinococcosis, cenurosis and сycticercoses); the implementation of measures for chemoprophylaxis of helminthiasis in certain branches of animal husbandry, for example, in sheep breeding; by using anthelmintic-salt mixtures; the use of agents that increase the immunobiological protective properties of the animal body against helminthiases and other diseases through the widespread use of vitamins, micro- and macroelements and other biological stimulants.Keywords: helminths, helminthiases, deworming, molluscicides, anthelmintics, anthelmintic-salt mixtures, immunostimulants
О. М. Yakubchak, Т. V. Таran, S. V. Міdyk, V. V. Danchuk, N. Ya. Dudchenko, L. M. Vygovska
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.004

Abstract:
The percentage of fatty acids in cow's milk is an indicator of its naturalness, which is now relevant given the prevalence of adulteration of milk and dairy products with vegetable fats. The aim was to study certain quality indicators and fatty acid composition of milk obtained in the conditions of NULES of Ukraine "Agronomic Research Station". Materials, methods. Collective chilled milk obtained at different times of the year on the basis of ULYABP APC NULES of Ukraine was studied. Organoleptic and chemical parameters as well as fatty acid composition of milk were determined. Extraction of lipids from milk was performed by the method of Folch. Analysis of LCD methyl esters was performed on a Trace GC Ultra gas chromatograph (USA) with a flame ionization detector. Identification of fatty acids was performed using a standard sample Supelco 37 Component FAME Mix. Quantitative evaluation of the fatty acid spectrum of milk fat lipids was performed by the method of internal normalization, determining their content in percent. The study was performed in 2 parallels. Statistical processing of experimental data was performed by conventional methods of variation statistics. The probability of the difference in indicators was assessed by Student's t-test. Differences between the compared indicators were considered significant at the significance level P≤0.05. Results and Discussion. Studies have shown that milk is natural because its fatty acid composition corresponds to the fatty acid composition of milk fat. - 1.7, spring - 1.6 times. In summer and autumn, the content of unsaturated and monounsaturated fatty acids in milk increases, in autumn - polyunsaturated, which is due to the presence in the diet of cows green mass and corn silage and the lack of concentrated feed and vitamin and mineral supplements. The obtained results can be used as a basis for compiling a diet to obtain milk with optimal content and ratio of fatty acids, which can become competitive in the market of raw milk for the production of milk and dairy products of improved chemical composition, dietary, functional products, baby food.
S. A. Tkachuk, I. V. Yatsenko, L. V. Tkachyk
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.002

Abstract:
It is known that feed additives with Omega -3 improve the productivity and health of pigs. Evaluation of morphological and biochemical parameters of pig blood with the use of feed additives is a necessary study to establish scientific evidence of the effects on the body of additives used in pig feeding.The article presents scientific research to determine the morphological and biochemical parameters of blood in young pigs and pigs for fattening for the use in the diet of organic feed additives Lg-Max and Sel-Plex.Feed additives Lg-Max together with Sel-Plex, was introduced as part of a premix to animal feed of the experimental group, taking into account the need of animals in Omega-3 unsaturated fatty acids (daily requirement of pigs in Omega-3 is 672 mg. In 1 g of experimental feed additive contains 353 mg of Omega-3), and the drug Sel-Plex – in the amount of 0.5 mg / kg.According to the results of the study, it was found that the studied morphological parameters of pig blood were within physiological limits according to age.Biochemical parameters in the serum of the experimental group of pigs in comparison with the control of the corresponding age are in the following dynamics: in pigs of 45 days of age probably increases – the concentration of globulins; α 2-globulins and β-globulins, and probably decreases the content of AST; in pigs 120 days of age is likely to increase – the content of total protein and magnesium; probably decreases – the content of cholesterol and alkaline phosphatase; in 155-day-old pigs, the concentration of calcium probably increases, and the concentration of cholesterol probably decreases.
N. B. Kolych, National University of Life and Environmental Sciences of Ukraine, N. Hudz
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.03.005

Abstract:
In recent years, there is a tendency to increase the incidence of the disease in dogs with signs of diarrhea, not only in Ukraine, but also in Europe. In general, diseases with the manifestation of such specific clinical sign as diarrhea can be caused by viruses, bacteria and helminths. In general pathology of dogs, enterovirus infections of puppies occupy a leading place. The practice of specific prophylaxis suggests that in the etiological aspect diseases emerging with this symptom can be caused not only by parvo-, but also corona- and rotavirus enteritis. These diseases are extremely dangerous for puppies and miniature dogs, due to rapid dehydration, and as a result, death.The article presents the results of a study on histological changes in organs and tissues of dogs with coronavirus infection. A histological study of pathological material sampled from 6 dogs of various breeds and sex, aged from 2 to 6 months, died from diarrheal syndrome was carried out. The presence of the coronavirus was confirmed in fecal samples by polymerase chain reaction.The most expressed injuries and typical changes in all dead dogs we recorded in the small intestine, namely in the jejunum and ileum, regional lymph nodes, and also in the spleen.Morphological manifestations of coronavirus infection in dogs at macroscopic level are the following signs: the presence of exudative inflammation in the small intestine in form of serous-fibrinous jejunoileitis, hyperplasia and serohemorrhagic lymphadenitis of the mesenteric lymph nodes, multiple foci of hemorrhage in the parenchyma of the spleen and serous membrane of the small intestine, passive venous hyperemia of the liver and kidneys, dilatation of the right ventricle of the heart, pulmonary edema, cachexia and dehydration of the body.At the microscopic level we registered the following: serofibrinous jejunoileitis, hyperplasia of the lymphoid nodules of the spleen and lymph nodes, hemorrhagic infarctions in the spleen, hyperplasia of individual and aggregated lymphoid nodules of the mucous membrane in the small intestine, degenerative processes in the parenchyma of the liver and kidneys.
Yakubchak О. М., National University of Life and Environmental Sciences of Ukraine, Таran Т. V., Ushkalov V. О., Міdyk S. V.
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.02.003

Abstract:
Introduction. Ukraine is actively implementing safety legislation and certain indicators of food quality, in particular raw milk, to the requirements of the European Union. Modern requirements for raw milk require careful analysis of hygienic indicators. Materials and methods. Raw milk materials supplied to Bila Tserkva Dairy Plant LLC and PJSC Vita Dairy Plant of Kyiv Region were studied. The number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAM) and the species composition of the milk microflora, in particular, bacteria of the genus Salmonella, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli bacteria (psychotrophs) and Physico-chemical methods were used to determine: density, mass fraction of dry matter, somatic cell content, acidity, purity group, mass fraction of protein and fat. Results and Discussion. According to research, the quality of farm milk is an order of magnitude better than milk obtained from private farms, in particular, KMAFAM. The technology of obtaining farmer's milk ensures its production of the highest and first grades, while the milk obtained in the conditions of private peasant farms – the first grade and non-grade. According to physicochemical parameters, milk obtained under different conditions did not differ significantly. Microbiological parameters differed significantly. The average number of MAFANM milk from private farms was 4361.25±241.15, which is 12.6 times higher than the number of MAFANM even milk of the first grade obtained in a dairy farm. Irrespective of the season and conditions of raw milk production, all tested samples met the requirements of the current DSTU for the absence of bacteria of the genus Salmonella in 25 cm3, Staphylococcus aureus, 0.1 cm3 and Listeria monocytogenes, 25 cm3. Bacterial bacteria were not detected in farm milk during the year, in contrast to milk from private farms, where this group of bacteria was detected in spring and autumn (20% of cases). Both in farming and in milk from private farms, a group of mesophilic microorganisms prevailed over spore-forming and psychrotrophic ones. However, their number was different. Prospects for further research are to determine the sources of various types of microorganisms in raw milk and to develop procedures to eliminate the possibility of contamination of milk with foreign microflora. Key words: raw milk, farm milk, milk from private farms, milk microflora, quality indicators
Stybel V. V., Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Gutyj B. V., Said W. S., Kubiak K., Jankowski M., Maksymovych I. A., Guta Z. A., Martyshuk T. V., Karpovskyi V. I., et al.
Ukrainian journal of veterinary sciences, Volume 12; https://doi.org/10.31548/ujvs2021.02.001

Abstract:
The article presents the results of studies of the effect of fenbensyl and fenbendazole on the antioxidant status of dogs in the experimental infestation of the pathogen toxocariasis. The experiments were performed on 18 dogs, two and four months old. Three groups of six animals in each were formed: control and two experimental groups. Puppies of all groups were experimentally infected with the pathogen toxocariasis at a dose of 5,000 invasive T. canis eggs per kg of body weight. The control group of dogs was as untreated control. Puppies of the first experimental group were fed with the drug “Fenbendazole” at a dose of 150 mg per 3 kg of animal weight once a day for three days in a single doze. Puppies of the second experimental group were fed with the drug “Fenbensyl” (TU U 00492990-027: 2020 The drug “Fenbensyl”) at a dose of 350 mg per 3 kg of animal weight once a day for three days. While studying the activity of the enzymatic link of the antioxidant defense system, namely catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, as well as non-enzymatic link: the level of reduced glutathione in animals of experimental groups found an increase in the activity of these indicators. It should be noted that the use of the drug “Fenbensyl” in infected dogs contributed to a better normalization of the antioxidant system in animals than the use of the drug “Fenbendazole”. The use of fenbensyl in infected dogs contributed to a more likely increase in the antioxidant status of the dogs of the second experimental group, as this drug includes milk thistle, which exhibits antioxidant properties due to the presence of silymarin, which restores damaged liver cell cells. Studies confirm the effectiveness of the use of milk thistle in the drug “Fenbensyl” in dogs with the development of toxocariasis invasion to activate the protective systems of their body.
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