MedPulse International Journal of Biochemistry

Journal Information
ISSN / EISSN : 2550-763X / 2636-4573
Total articles ≅ 112

Latest articles in this journal

MedPulse International Journal of Biochemistry, Volume 19, pp 34-37;

Background: Acute pancreatitis is a sudden inflammation that lasts for a short time. It may range from mild discomfort to a severe, life-threatening illness. Methods: It was Prospective and observational study. All the patients admitted in medicine department and ICU and diagnosed as acute pancreatitis. Study period was January 2018 to September 2019. And sample size was 30. Investigation was done in the department of Biochemistry. Results: The prevalence rate of acute pancreatitis was higher in male than female with a ratio of 1.72:1 and we found maximum patients were in the age group of 41-50 years. While analysing the biochemical parameters we found very obvious a high lipase, amylase and triglyceride level in the study subjects accounting most of the cases as these biochemical parameters are directly related to acute pancreatitis. Conclusion: Pancreatic necrosis may lead to the development of pancreatic pseudocysts or tissue abscess, common complications associated with pancreatitis. A better understanding of the nature of cardiac dysfunction in this setting may allow more accurate diagnosis, prognostication, and management.
MedPulse International Journal of Biochemistry, Volume 19, pp 12-15;

Background: The thyroid hormones are involved in controlling metabolism, growth and functions of various organ systems of our body. Hypothyroidism, a very common endocrinopathy features reduced production of thyroid hormones. In hypothyroidism we encounter abnormal circulating lipid fractions which is termed as ‘Dyslipidemia’. The coexistence of dyslipidemia and hypothyroidism is associated with development of atherosclerosis. Aim: This research is undertaken to assess the potential of TC/HDL-C ratio to disclose dyslipidemia in hypothyroidism. Methodology: The study consists of two major groups, the ‘Cases’ comprising of Fifty hypothyroid patients and ‘Controls’, with fifty age and gender matched healthy adults. The Cases are further subdivided into Twenty ‘Known cases’ of Hypothyroid patients, already receiving treatment and thirty ‘New cases’. The ‘New cases’ sub group further is subdivided as Twenty-one ‘Overt hypothyroids’(OH) and nine ‘Subclinical Hypothyroids’ (SCH). The Biochemical parameters estimated in all subjects of the study includes, Serum TSH, T3, T4, Total Cholesterol (TC) and High density lipoprotein-cholesterol(HDL-C). TC/HDL ratio was then calculated . Result: The study shows a significant elevation (p = 0.05) in the TC/HDL-C ratio in hypothyroid patients (3.67 ± 1.02) when compared to healthy controls (3.29 ± 0.95). TC/HDL-C ratio is more elevated in OH (4.01 ± 1.28) than in SCH (3.46 ± 0.92). The levels in ‘New cases’ sub-group and OH sub sub group have significantly higher ratio than in healthy controls (p = 0.02 and p = 0.01 respectively). TC/HDL-C ratio value in “known cases’ group who are those hypothyroid patients on Levo-thyroxine therapy is (3.40 ± 0.61) less than those who are newly diagnosed. Conclusion: TC/HDL-C ratio is not only raised in hypothyroid patients but it is seen increasing with severity and prolonged duration of disease. The calculation of TC/HDL-C ratio should be routinely put into practice to detect hypothyroid patients with dyslipidemia.
Joel Sabu
MedPulse International Journal of Biochemistry, Volume 19, pp 22-25;

Background: Alteration in levels of copper, iron and zinc variate the enzymatic actions in our body and thereby play a major role in etiopathogenesis of oral carcinogenesis. Aim of the study: The present study was mainly aimed to estimate, compare and correlate the serum levels of copper, iron and zinc with haematological parameters in oral cancer patients in comparison to normal controls. Methods: The collected serum of cases and controls were analyzed by using standard spectrophotometric methods in spectrophotometer analyzer and the data obtained was analysed and represented as Mean ± SD, mean difference was analyzed by Student’s T-test and Chi- square test for significance and strength of association by Karl Pearson’s correlation using SPSSv23 software. Results: Mean serum level of iron was significantly lower and the levels of zinc and copper in patients with oral cancerous lesions were significantly higher than that of healthy individuals. Pearson’s r data analysis, revealed a significant negative correlation between iron with platelets, and MCHC (Mean Corpuscular Haemoglobin Concentration). A significant positive correlation was seen between iron with haemoglobin and lymphocytes; between copper with leucocytes. Conclusions: The serum levels of zinc and copper in group I were significantly higher when compared to controls. There was a significant lower serum level of iron in Group-I when compared to controls. A significant correlation was seen in between serum trace elements and haematological parameters in oral cancer. This suggests the involvement of trace elements in variations of haematological parameters in the pathogenesis of oral carcinogenesis.
X Lourdes Sandy
MedPulse International Journal of Biochemistry, Volume 19, pp 26-29;

Background: The most common endocrine disorder is hypothyroidism which accounts to 11%. Thyroid hormones have a wide array of functions such as physiological growth and development of skeletal system, maintenance of basal metabolic rate and regulation of various metabolisms, including mineral metabolism. Nowadays due to its direct action on bone turn over, thyroid hormones are considered to have an important role on bone mineral metabolism. Thyroid disorders are important cause for secondary osteoporosis. So the present study was done to know the levels of bone minerals, calcium and phosphorus in hypothyroidism and its relation with thyroid hormone levels. Methods: A case-control study was conducted on 30 hypothyroid patients and 30 euthyroid healthy controls in the age group of 20-60 years. Blood samples were collected from all the study population. Serum total triiodothyronine, total thyroxine and TSH by Enzyme-Linked Immunosorbent Assay, Serum calcium by Arsenazo III method, phosphorous by ammonium molybdate method were estimated. Results: Serum calcium levels in cases was found to significantly reduced when compared to controls (p<0.001). Serum phosphorous levels also showed considerable elevation in cases when compared to controls (p<0.001). There was a significant negative correlation between TSH and serum calcium in cases. Conclusion: The present study indicated the important role of reduced thyroid hormone status on bone mineral metabolism. This study concludes that serum calcium was significantly reduced and phosphorus levels were significantly increased in hypothyroid patients when compared to euthyroid control subjects. So frequent monitoring of serum calcium and phosphorus in hypothyroid patients would reduce the burden of bone pathologies.
MedPulse International Journal of Biochemistry, Volume 18, pp 39-42;

Background: Iron deficiency anemia (IDA) is most common cause of anemia in pregnancy. In order to prevent this iron supplementation is routinely practiced as a prophylactic measure in pregnant women all over. The biochemical parameters assessed in IDA comprise an iron profile evaluation which consists of estimation of serum ferritin, serum iron, total iron binding capacity(TIBC), serum transferrin levels and calculation of transferrin saturation. These biochemical parameters are subjected to variations due to maternal adaption phenomenon. Aim: In the current study we have chosen three iron indices, serum iron, serum TIBC and transferrin saturation percent to note their performance in diagnosing and monitoring the response to iron therapy in pregnant women. Methodology: The study population are thirty- six pregnant women in their early second trimester, who are diagnosed with mild iron deficiency anemia (Hemoglobin between 9 and 11 g %). Iron parameters, serum iron, serum TIBC and transferrin saturation levels were analyzed in these women. They are then given oral iron preparation in the form of Ferrous sulphate for a period of twelve weeks. After this the Hemoglobin level, serum iron, TIBC and transferrin saturation levels are re-analyzed in these women. Results: The hemoglobin levels increased (p= 0.002). as expected after oral iron intake. Serum iron levels improved from 58.19±39.07 to 64.78±34.96 μg/dl. Serum TIBC value before supplementation 234.22±134.49 increased to 437.33±94.95 after, which contradicts the expected pattern seen in response to therapy in general population. Similarly absurdity prevails in transferrin saturation index levels which dropped from 36.8 ± 31.8 to 16.3 ± 10.6. Conclusion: The iron status during pregnancy is highly influenced by the maternal changes. And a blind interpretation of the report can lead to erroneous diagnosis. The interpretation of values should be based on the trimester specific reference ranges during pregnancy.
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