Journal of Aquaculture and Fish Health
ISSN / EISSN : 2301-7309 / 2528-0864
Current Publisher: Universitas Airlangga (10.20473)
Total articles ≅ 129
Latest articles in this journal
Journal of Aquaculture and Fish Health, Volume 10, pp 9-16; doi:10.20473/jafh.v10i1.18234
Digestibility is food substances from feed consumption that are not excreted into the feces, and the difference between food substances consumed and those excreted in the feces is the amount of food that can be digested. Information about the digestibility value of food is very important as a basis in assessing the quality of food and designing fish feed rations.This research was conducted to determine the level of the feed digestibility in nirvana tilapia (Oreochromis niloticus) feed with a combination of fish meal and maggot flour. This research was conducted at the Hatchery Campus of BAPPL STP Serang, Banten from September-December 2019. This study used a Completely Randomized Design (CRD) with 5 treatments and 3 replications. There were 225 fish used in the testing with an average weight of 0.16 grams. The culture media used was a plastic container with a size of 60 x 41 x 34 cm. Feeding was carried out for 50 days, with a frequency of twice a day, and the dose of feeding was 3% of the biomass.The results showed the total digestibility parameter of feed, protein digestibility of feed and the highest protein retention in the treatment of 50% of the combination feeds , with the highest total digestibility value of 56.97 ± 3.41 and the lowest of 42.08 ± 0.42, the highest protein digestibility with a value of 87.33 ± 0.95and the lowest of 83.11 ± 1.30 and the highest protein retention with a value of 14.83 ± 0.22and the lowest of 11.69 ± 1.05.
Journal of Aquaculture and Fish Health, Volume 10, pp 85-94; doi:10.20473/jafh.v10i1.20876
Pangasius catfish is freshwater fish for consumption that can be cultivated through fish farming. In order to produce optimal profits, Pangasius catfish farming must pay attention to the use of inputs in the form of seeds, feed and electricity. The purpose of this study was to analyze the optimization of Pangasius catfish production in Tondo Lestari fish cultivator group, Pagersari Village, Tulungagung Regency. Data collection techniques were in the form of interviews, observation and documentation. The data analysis used was linear programming using the POM-QM for Windows 5 software tool. The Pangasius catfish farming activities of the Tondo Lestari fish cultivator group have been optimal because they resulted in a low production difference between factual and optimal conditons of 3,05 kg or a profit of Rp. 10,502. . The optimal production of Pangasius catfish in a pond size of 200 m2 was 3,173.21 kg and the optimal production in a pond size of 300 m2 was 4,839.83 kg. Therefore, it can be concluded that Pokdakan Tondo Lestari can take advantage of additional feed and electricity input by reducing the use of seed input by 1.487 heads to increase profits.
Journal of Aquaculture and Fish Health, Volume 10, pp 1-8; doi:10.20473/jafh.v10i1.17088
Euchema cottoni or Kappaphycus alvarezii is one type of seaweeds that is widely cultivated in Indonesia. Water pollution caused by the accumulation of Pb heavy metals will affect the seaweed to grow and develop. This study is aimed to determine the tannin levels in K. alvarezii and the relationship of Pb heavy metal pollution level with tannin levels in K. alvarezii in different waters. This study used an observational experiment, and samples were taken from two different locations with 4 stations in each. From each sample in both locations, K. alvarezii was taken and was then measured for the tannin levels, water quality, and heavy metals (Pb) in water and in K. alvarezii. The results were analyzed at the Laboratory of PT. Sucofindo and the Laboratory of Testing Service Unit, Airlangga University Surabaya. The data were analyzed using a statistical test in the form of a t-test to determine the differences in samples in both waters, and a C-square test was used to determine the relationship between PB and tannin levels in K. alvarezii. The results showed that the level of Pb heavy metal pollution in the two waters did not show a significant difference due to water quality which was quite similar in both locations. Moreover, the tannin content in K. alvarezii in Bluto waters (0.053±0.004%) was higher than that in Talango waters (0.020±0.009%). Meanwhile, a t-test showed a significant difference in seaweed tannins in both waters. The relationship between Pb heavy metal pollution and tannins of seaweed in Bluto and Talango waters was that they did not affect each other, and tannin concentration was more influenced by chemical oxygen demand (COD), salinity of both waters, and the cultivation methods used.
Journal of Aquaculture and Fish Health, Volume 10, pp 17-24; doi:10.20473/jafh.v10i1.18102
In intensive shrimp culture, oxygen consumption of shrimp is an important indicator that greatly affects the physiological condition of shrimp as a reared organism. The purpose of this study was to dynamically determine the oxygen consumption of shrimp in intensive culture as well as the variables of water quality and shrimp growth. This research was conducted with the concept of ex-post facto causal design in intensive aquaculture ponds during the shrimp cultivation period. During the shrimp culture period, the rate of oxygen consumption of shrimp is inversely related with the sigmodial average increase in shrimp body weight. Meanwhile, based on the simulation analysis with the dynamic modeling concept, it is shown that oxygen consumption was linear to the dynamics of average daily gain and inversely proportional with the increasing rate of shrimp biomass in the ponds. In addition, oxygen consumption rate of shrimp in intensive ponds had a close relationship with water salinity and total organic matter. In conclusion, dynamically, the fluctuation of oxygen consumption rate and average daily gain of shrimp in intensive culture are closely related to the stability of the water quality conditions on the shrimp habitat.
Journal of Aquaculture and Fish Health, Volume 10, pp 75-84; doi:10.20473/jafh.v10i1.19567
Chinese pond shell, Sinanodonta (Anodonta) woodiana (Lea, 1834), is a freshwater bivalve that has essential ecological and economic functions. The microstructure of the nacre is of great interest and is the main attraction for the development of pearl farming. This study aims to describe the microstructure and composition of biomineral elements of the nacre at several shell sizes of S. woodiana. The shell is cut with a small forcepon the ventral margin with a size of 3-5 mm for Scanning Electron Microscpy (SEM). SEM images display that a shell layer consists of periostracum, prismatic and nacre layers. The surface of the nacre layer is an irregular or labyrinth patterned. The nacre tablets are hexagonal, glued to each other, so the nacre tablets become polygonal. Moreover, the microstructure of the nacre tablets is like a brick wall, and the thickness of tablets from 0.43 μm to 0.59 μm. The composition of the biomineral elements are C, O, Ca, and the mineralization mechanism is under the control of aquatic environmental factors that help the process of microstructure formation in nacre.
Journal of Aquaculture and Fish Health, Volume 10, pp 109-116; doi:10.20473/jafh.v10i1.23424
Scaffold, as one of the components for bone tissue engineering, requires formulated biomaterials that are both structurally and compositively similar to bone composition. Among others, chitosan, gelatin and chondroitin sulfate are known as potential candidates for scaffold composites that can be easily obtained from waste-based resources. This study aims to investigate the cytotoxicity of different scaffold composition and concentration regimes derived from waste-based chitosan, gelatine and chondroitin sulfate, in vitro. The composition regimes used were (Chitosan : Gelatin : Chondroitin Sulfate) 50 : 50 : 0 (A); 50 : 40 :10 (B); 50 : 35 : 15 (C); 50 : 30 : 20 (D); 50 : 25 : 25 (E). Meanwhile, the final concentrations of scaffold used were 2000, 1000, 500, 250, 100, 10 and 0,1 mg/ml. The different compositions and concentrations of scaffold was tested against Hepatocellular Carcinoma (Huh7it / Human Hepatocyte It). After 48-hour incubation in the scaffold solution, the percentage of cell viability was evaluated using 3-(4,5-dimethylthiazol-2yl)-5(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTT) assay. The result shows that there is no difference observed among different scaffold compositions on the cell viability (p > 0.05). However, different concentrations of scaffold show significant differences in cell viability in composition C and E (p < 0.01), suggesting possible dose- dependent effect of scaffold on cell viability. Overall, all the waste-based scaffold compositions show no toxicity against the Hepatocellular Carcinoma cells as exhibited by the cell viability that is above 70%, at least with the concentration up to 2000 mg/ml.
Journal of Aquaculture and Fish Health, Volume 10, pp 34-45; doi:10.20473/jafh.v10i1.18926
Eyestalk ablation inflicted upon crustacean is generally performed in captivity to induce ovarian maturation. However, ablation is a hurtful action that may impose physiological stress and even affects survival. The immunologic repercussions of the ablation have not been well understood and thus this study aims to analyze the effect of ablation on immunologic variables through comparing the ablated Litopenaeus vannamei with those of unablated shrimps (control). Unilateral left eyestalk ablation was inflicted on shrimp samples. Shrimp immune responses were observed based on the Total Haemocyte Count (THC), Differential Haemocyte Count (DHC) composing of hyaline cell (HC) counts and granular cell (GC) counts, glucose and uric acid levels, along with the weight gains of shrimps. Observed parametres were taken twice from 10 shrimps for each of the observed and control samples (i.e., on 6 h and 5 d elapsed time). The results show that the ablated shrimps group, after 6 h elapsed time, experienced significant decrease in THC (3.12 ± 0.75 x 106 cells ml-1), but recovered after 5 days (3.89 ± 0.96 x 106 cells ml-1) and were not significantly different from the control (5.16 ± 2.20 x 106 cells ml-1). Meanwhile, glucose level show significant decrease over 6 h and 5 d post ablation (108 and 93 mg dL-1, respectively) compared to the resulting responses from the control (35-36 mg dL-1). Differences in DHC for all cell types as well as uric acid in hemolymph were not significant between the observed and the control samples. Moreover, the ablated treatment did not affect the growth of vannamei after 5 d post ablation. The results revealed that changes occur on the physiological and immune parameters of shrimps due to the eye ablation treatment as shown by the decrease in the THC and glucose levels, especially within the early moments post treatment. However, at the end of the observation (5 d post ablation), shrimps exhibit signs of recovery from stress indicated by an increase in THC close to normal.
Journal of Aquaculture and Fish Health, Volume 10, pp 66-74; doi:10.20473/jafh.v10i1.20905
Swordfish (Xiphias gladius) is a migratory fish commercially exploited due to high export value. Presence of parasites in fish lead to economic losses in the export market and public health issues. This study was conducted to identify the parasite larvae inhabiting swordfish and to determine its phylogenetic origin using ribosomal subunit gene sequence. Parasite samples were extracted from swordfish muscles and five larvae belong to Molicola genus, confirmed by scolex morphology, were used for genomic DNA extraction. Polymerase chain reaction (PCR) was performed to amplify 18S and 28S ribosomal RNA (rRNA) subunit genes followed by Sanger sequencing. DNA sequences were edited by BioEdit software and assembled by CLC genomics version 8.0. Consensus sequences were aligned with NCBI blast to determine the species status. Isolated larval sequences were best aligned with genus Molicola followed by genus Gymnorhyncha. Out of the two published Molicola rRNA gene sequences, 99% identity was observed with Molicola sp. HP5 isolate from Indonesia. Due to lack of sequence data on other Molicola species (except M. thyristes) for comparison, our sequences were published as Molicola sp. Sri Lankan isolates. This is the first record of Molicola sp. in swordfish from Sri Lanka and the results will enhance the knowledge on the distribution of Molicola species while contributing to expand the genetic information on rRNA coding sequences.
Journal of Aquaculture and Fish Health, Volume 10, pp 95-100; doi:10.20473/jafh.v10i1.22222
Marketing is the main problem felt by salt farmers in East Lombok Regency, especially during the harvest season, which has an impact on the conversion of the salt pond land to cultivation land. This research aims to: 1) identify salt marketing channels in East Lombok Regency, 2) develop salt marketing strategy in East Lombok Regency. This research uses qualitative methods and the data will be analyzed by SWOT analysis.Efficient salt marketing occurs when the marketing channels include producers (salt farmers)-cooperatives /micro, small and medium enterprises-consumers, because it can shorten marketing channels and-reduce operational costs. Marketing of salt in East Lombok Regency can be carried out with several strategies, namely: 1) Maximizing the role of cooperatives and micro, small and medium enterprises in salt marketing, 2) Improving the quality of human resources in managing the salt business, 3) Formulating regulations on salt trading, and 4) Providing production technology training to salt farmers and salt micro, small and medium enterprises.
Journal of Aquaculture and Fish Health, Volume 10, pp 56-65; doi:10.20473/jafh.v10i1.20794
Fish farming including freshwater crayfish still has a dependency on the availability of fish meal as the main source of protein in feed. The purpose of this study was to evaluate alternative protein sources including poultry by-products, feather meal, lupine flour, soybean meal and meat, and bone meal for smooth marron (Cherax cainii) freshwater crayfish feed. A total of 150 juvenile marrons (9.09 ± 0.21 g) were kept for 90 days and distributed into 15 rearing tanks consisting of five treatments with three replications. The results indicated that there was no significant difference in the increase in biomass, survival rate, moulting rate (weight), feed efficiency ratio, and feed digestibility (P> 0.05). However, marron juvenile fed with a protein source of poultry by-products meal had the highest specific growth rate (0.31 ± 0.05 g / day). In addition, marron fed with fish meal protein resulted a significant increase in carapace length (P 0.05). Overall, the results of this study indicate that the general growth performance of marron is not affected by the replacement of fish meal in the feed. Alternative protein materials can be used as a protein source to replace fish meal in marron feed so that the use of fish meal can be reduced.