Jurnal Iktiologi Indonesia

Journal Information
ISSN / EISSN : 16930339 / 25798634
Current Publisher: Masyarakat Iktiologi Indonesia (10.32491)
Total articles ≅ 67
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Renny Kurnia Hadiaty, Mf Rahardjo, Gerald R Allen
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.446

Abstract:Indonesia is one of the biggest archipelago country in the world, These islands lined up, stretching from Sabang to Merauke. Some government institutions studied the number of islands in Indonesia. In March 2nd 2017 President of Republic Indonesia issued Keppres No. 6 2017 about 111 numbers of outer islands. Two of the islands belongs to Bengkulu Province, i.e Enggano and Mega islands. Stretching in an east-west direction embracing nearly 18,000 islands, the Indonesian Archipelago features a seemingly endless array of marine habitats. The country is the global leader with regards to area occupied by coral reef, estimated at 51,090 km2. The total number of coral reef fishes around 2600 species. The highest diversity of coral reef fishes with about 1766 species is Papua Barat. The dominant family is Gobiidae with 405 species, Labridae about 200 species and Pomacentridae with about 176 species. The islands in Indonesia have a unique history and inhabit by numbers of endemic fish species, i.e Muna island, Aru islands, and Enggano island. LIPI conducted an expedition in Enggano in 2015, the result indicated some undescribed species there. The new species found in Indonesia since a long time ago, started on 16 century up to now. The research on freshwater fish in Indonesia found 66 new fish species. Until now there are still a lot of unexplore waters in Indonesia, and there are still awaiting undescribe fish species to be named. Fish conservation in the small islands need to be done, as most of them migrate to the sea. Some aphidromous species growth and reproduction in freshwaters but the juvenile migrate downstream towards the sea, spreading at sea, metamorphosis phase they enter upstream migration and settlement in the freshwater.AbstrakIndonesia merupakan negara kepulauan yang terbesar di dunia, pulau-pulau ini berjajar, membentang dari Sabang sampai Merauke. Kajian jumlah pulau di Indonesia telah diteliti oleh beberapa lembaga di negeri ini. Sebagai negara kepulauan, Indonesia seolah-olah merupakan habitat laut yang tak berujung. Terumbu karang negeri ini sekitar 51.090 km2 merupakan yang terluas di dunia. Jumlah total spesies ikan di perairan terumbu karang Indonesia sekitar 2600 spesies. Wilayah tertinggi keragaman jenisnya adalah perairan Papua Barat dengan 1766 spesies. Famili yang terbesar adalah Gobiidae 405 spesies, selanjutnya Labridae 200 species dan Pomacentridae 176 spesies. Banyak pulau mempunyai riwayat unik dan dihuni oleh spesies-spesies endemik, diantaranya Pulau Muna, Pulau di Kepulauan Aru, juga Pulau Enggano. Hasil penelitian LIPI di P. Enggano mengindikasikan adanya beberapa jenis baru ikan. Penemuan spesies baru telah berlangsung lama, sejak penelitian iktiofauna di Indonesia dimulai pada abad 16 sampai saat ini. Hasil penelitian ikan air tawar di perairan Indonesia telah mendapatkan 66 spesies baru, sampai saat ini masih banyak wilayah perairan belum diteliti dan kemungkinan besar ada spesies baru yang menanti diungkap...
Erlangga, Zulfikar, Hariyati
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.431

Abstract:Slow growth is obstacles to achieving the target of goldfish production (Carassius auratus auratus). The process of metabolism and growth in fish is influenced by hormonal factors. This research was conducted in February-March 2016 at Hatchery and Aquaculture Technology Laboratory Aquaculture Study Program of Faculty of Agriculture University of Malikussaleh. This study aims to examine the effect of thyroxine (T4) and hormone recombinant Growth Hormone (rGH) hormone by immersion method with different doses on growth and survival of goldfish larvae. The experimental method using a complete non factorial randomized design with four three replication treatments. The treatment is: A= control, B= T4 0.05 mgL-1 + rGH 5 mgL-1, C= T4 0.1 mgL-1 + rGH 10 mgL-1, D = T4 0.15 mgL-1 + rGH 15 mgL-1. Result of research with giving of recombination of hormone T4 and hormone rGH with different dose have an effect on to decrease of yolk volume, egg yolk absorption rate, growth and survival with p value
Muhammad Sami Daryanto, Odang Carman, Dinar Tri Soelistyowati Soelistyowati, Rahman
Jurnal Iktiologi Indonesia, Volume 19, pp 43-52; doi:10.32491/jii.v19i1.405

Abstract:The variation in the maximum number of nucleoli per cell in diploid and tetraploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 in attempts to verify the validity of nucleoli counting as an indirect method for polyploidy identification in fish. The aims of this research is to determine ploidy level of striped catfish based on maximum number of nucleoli per cell. Diploid striped catfish was produced by fertilizing eggs without thermal-shock and tetraploid fish was obtained by fertilizing eggs and applying thermal-shock induction with 4oC for 25 minutes at zygotic age 28 minutes after fertilization prior to first cleavage stage of zygote. The hatching rate of diploid group and tetraploid group were 81,35±0,73% and 3,39±1,78% and survival rate during 15 days rearing were 88,67±5,25% and 83,33±5,73%, respectively. The frequency of one, two, three, and four nucleoli per cell were counted based on each sample observation of 450-550 cells. Cells of diploid individuals had one, and maximum two nucleoli per cell, while tetraploid there were one, two, three, and maximum four nucleoli per cell. Anomaly in the silver-stained appearance at the maximum number of three nucleoli per cell indicated tetraploid individuals as verified by chromosome counting method. Ploidy level determination of striped catfish using the number of nucleoli per cell has a potential for rapid identification.AbstrakPenentuan variasi jumlah maksimum nukleolus per sel pada individu diploid dan tetraploid patin siam Pangasianodon hypophthalmus Sauvage, 1878 dilakukan dalam upaya verifikasi ketepatan penghitungan nukleolus sebagai metode tidak langsung dalam identifikasi ikan poliploid. Penelitian ini bertujuan menentukan tingkat ploidi ikan patin siam berdasarkan jumlah maksimum nukleolus per sel. Diploid patin siam dihasilkan melalui fertilisasi buatan antara telur dan sperma tanpa kejutan suhu sedangkan tetraploid patin siam dihasilkan melalui fertilisasi antara telur dan sperma serta di induksi menggunakan kejutan suhu 4oC dengan durasi kejutan 25 menit pada umur zigot 28 menit setelah fertilisasi sesaat sebelum mitosis I. Persentase penetasan kelompok perlakuan diploid 81,35±0,73% dan kelompok perlakuan tetraploid 3,39±1,78%. Sintasan kelompok perlakuan diploid 88,67±5,25% dan kelompok tetraploid 83,33±5,73% selama 15 hari pemeliharaan. Frekuensi satu, dua, tiga dan empat nukleoli per sel dihitung berdasarkan pengamatan 450-550 sel setiap individu yang diamati. Sel individu diploid memiliki satu dan maksimum dua nukleoli per sel, sedangkan individu tetraploid memiliki satu, dua, tiga, dan maksimum empat nukleoli per sel. Anomali muncul melalui pewarnaan perak nitrat pada jumlah maksimum tiga nukleoli per sel menunjukkan individu tersebut tetraploid yang diverifikasi menggunakan metode penghitungan kromosom. Penentuan ploidi pada ikan patin siam menggunakan penghitungan jumlah nukleolus per sel memiliki potensi dalam identifikasi secara cepat.
Munawar Khalil, Yunidar, Mahdaliana, Munawwar Khalil, Rachmawati Rusydi, Zulfikar
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.434

Abstract:Introduction fish in Indonesia has been negatively impact to the water ecology, such as declining in the number of native fish species. Handling the negative impacts of introduced fish can be minimized by reducing fertility rates through the use of plant compound extracts. This study was conducted in May - June 2016 which aimed to assess the effectiveness of the papaya seed flour (Carica papaya L) for the reproductive function of introduction fish Oreochromis niloticus. The research design used in this study was completely randomized design, non-factorial with five treatments and three replications, namely A: control (without giving the flour), B: 40 mg, C: 50 mg, D: 60 mg, E: 70 mg of papaya seeds flour mixing in 100 grams of artificial feed. The fish sample used were 45 mature tilapia fish, 4-5 months old with 200 g in weight for female and 250 gram in weight for male. Parameters measured in this research were feed consumption level, fecundity, fertilization rate, hatching rate and sperm morphological structures. The results of this study indicated that the papaya seed flour gave the multiple effect on the sperm abnormality and decreased the motility level of sperm. Statistical analysis showed that the application of papaya seed flour gave significantly different effect (P 0.05. The lowest sperm motility rate was found in treatment E, which was 00.45 ".032 /second. The average eggs number in this study ranged from 994,33 to 1.416 grains, whereas the lowest fertilization level and the lowest hatching rate was in treatment E with the percentage 58.58% and 99.24%.AbstrakIkan introduksi di Indonesia telah menimbulkan beberapa dampak negatif terhadap ekologi perairan, diantaranya adalah menurunnya spesies ikan asli. Penanganan dampak negatif ikan-ikan introduksi dapat diminimalkan dengan cara menurunkan angka fertilitas melalui penggunaan ekstrak senyawa tumbuhan. Penelitian ini dilaksanakan pada bulan Mei – Juni 2016 yang bertujuan untuk menganalisis efektivitas tepung biji pepaya (Carica papaya L) dalam menurunkan fungsi reproduksi ikan nila Oreochromis niloticus. Rancangan penelitian yang digunakan adalah rancangan acak lengkap non-faktorial dengan lima perlakuan tiga ulangan yaitu A: Kontrol (tanpa pemberian tepung), B: Pemberian 40 mg, C: 50 mg, D: 60 mg, E: 70 mg tepung biji pepaya dalam 100 g pakan buatan. Ikan sampel yang digunakan adalah induk ikan nila sebanyak 45 ekor yang berumur 4-5 bulan dengan berat bobot tubuh 200 g untuk induk betina dan 250 g untuk induk jantan. Parameter yang diukur ialah daya konsumsi pakan, jumlah telur (fekunditas), tingkat pembuahan telur, tingkat penetasan telur, dan morfologi sperma. Hasil penelitian menunjukkan bahwa tepung biji pepaya memberikan efek terhadap abnormalitas sperma dan menurunkan sintasan sperma, dan penurunan tingkat pembuahan telur (fertilitas rendah). Analisis statistik menunjukkan bahwa pemberian tepung biji pepaya memberikan pengaruh yang berbeda nyata (P0,05). Angka motilitas...
Yoke Hany Restiangsih, Nur’Ainun Muchlis
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.349

Abstract:Coral fish is the one of the important fish resources in Indonesian waters, but the potential and exploitation rate needs to be obtained. Pink ear emperor is one of coral fish that many caught in Bangka. Research was conducted to determine biological aspect of pink ear emperor caught by fish traps, that includes length distribution, length-weight relationship, sex ratio, maturity stage, food habits, and length at first mature. This research was conducted from February 2014 to November 2015 with sampling location in Sungailiat fishing port. The observed aspects included fork length, weight, sex, gonad maturity and fish stomach contents was done monthly. The results showed the growth pattern are allometric negative, folk length ranged from 12 to 58 cm. The condition of sex ratio of males to females was 1:2,06. The gonade maturity stage of males and females were stage I, II, III, and IV for all months of observation. Stomach contained was shellfish, crap, shrimp and fish. Length at first capture(Lc) was 28 cm and length at first maturity(Lm) was 26 cm. Size length at first capture was bigger than the size length at first maturity, so the pink ear emperor at Sungailiat available to maintained the balance of fish stock on population.AbstrakIkan karang merupakan salah satu sumber daya yang penting di perairan Indonesia, namun tingkat potensi dan tingkat pemanfaatannya masih perlu untuk dikaji. Ikan lencam merupakan salah satu ikan karang yang banyak tertangkap di perairan Bangka. Tujuan penelitian ini mengkaji beberapa aspek biologi ikan lencam hasil tangkapan bubu, meliputi sebaran ukuran panjang, pola pertumbuhan, nisbah kelamin, dan ukuran rata-rata pertama kali matang gonad dan kebiasaan makan. Penelitian ini dilaksanankan di PPN Sungailiat pada bulan Februari 2014 – November 2015. Aspek yang diamati meliputi panjang cagak, bobot tubuh ikan, jenis kelamin, kematangan gonad, dan isi lambung ikan. Hasil penelitian menunjukkan pola pertumbuhan ikan lencam bersifat allometrik negatif. Sebaran ukuran panjang berkisar antara 12 – 58 cm. Nisbah kelamin jantan terhadap betina sebagai 1 : 2,06. Pada setiap bulan pengamatan tingkat kematangan gonad jantan dan betina berada pada stadia I, II, III, dan IV. Isi lambung ikan lencam selama pengamatan terdiri dari kerang-kerangan, kepiting, udang, dan hancuran ikan. Panjang pertama kali ikan tertangkap (Lc) sebesar 28 cm dan pertama kali matang gonad (Lm) sebesar 26 cm. Rata-rata ukuran pertama kali tertangkap lebih besar dbandingkan dengan ukuran pertama kali matang gonad sehingga ikan lencam di Perairan Bangka masih dapat mempertahankan keseimbangan stok dalam suatu populasi.
Nyoman Dati Pertami, M.F. Rahardjo, Ario Damar, I.W. Nurjaya
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.444

Abstract:Bali Sardinella (Sardinella lemuru Bleeker, 1853) was the main catch for fishermen around the Bali Strait waters. The unwell management of the catch effort and the unavailable of allowable catch size were suspected as the factors which made this fish became rare to find in Bali Strait nowadays. In addition, environmental factors were also thought to play a role, for example, the availability of food. This study aimed to reveal the food and feeding habit of Bali Sardinella in Bali Strait waters and their connectivity to the availability of natural food in nature. This study was held for 13 months, from May 2017-May 2018 in three areas of the Bali Strait waters, namely Pengambengan, Pekutatan, and Yeh Leh. Organisms found in the digestive tract of fish were identified until the genera level. The method used for feeding habit was the Important of Relative Index (IRI). Meanwhile, for determining the electivity of the food using the Index of Electivity. Based on the IPR, the food organisms with the highest value were Cyanophyceae (Trichodesmium) and Bacillariophyceae (Nitzshia and Chaetoceros). The main food varied depending on the season and fish size. Bali Sardinella were planktivorous and chose their food. Plankton which is abundant in the waters was not always found in large quantities in the digestive tract.AbstrakIkan lemuru (Sardinella lemuru Bleeker, 1853) adalah salah satu tangkapan utama nelayan di sekitar Selat Bali. Beberapa faktor yang disinyalir menjadi penyebab turunnya produksi ikan ini adalah upaya tangkap yang tidak terkelola baik pada masa lalu dan belum adanya batas ukuran yang boleh ditangkap. Selain faktor penangkapan tersebut, faktor lingkungan juga diduga berperan, misalnya ketersediaan makanannya. Penelitian ini bertujuan untuk mengungkapkan makanan dan kebiasaan makan ikan lemuru di perairan Selat Bali dan keterkaitannya dengan ketersediaan pakan alaminya di alam. Penelitian berlangsung selama 13 bulan, sejak Mei 2017-Mei 2018 di tiga area di perairan selat Bali, yaitu Pengambengan, Pekutatan, dan Yeh Leh. Organisme yang ditemukan di dalam saluran pencernaan ikan diidentifikasi hingga tingkat genera. Untuk kebiasaan makanan ikan, metode yang digunakan adalah Indeks Penting Relatif (IPR). Sementara itu, metode yang digunakan untuk menentukan makanan yang dipilih ikan adalah Indeks Pilihan Jenis Makanan. Berdasarkan nilai IPR, organisme makanan yang nilainya paling tinggi adalah dari kelas Cyanophyceae (Trichodesmium) dan Bacillariophyceae (Nitzshia dan Chaetoceros). Organisme yang paling banyak dimakan berubah-ubah bergantung kepada musim dan ukuran ikan. Lemuru adalah ikan planktivora dan memilih makanannya. Plankton yang melimpah di perairan belum tentu ditemukan dalam jumlah yang banyak dalam saluran pencernaannya.
Jusmaldi, Dedy Duryadi Solihin, Ridwan Affandi, Mf Rahardjo, Rudhy Gustiano
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.387

Abstract:Reproductive biology of silurid catfish O.miostoma(Vaillant 1902)as one of endemic species in Mahakam River East Kalimantan is not yet known. This study aimed to analysisreproductive aspect related to changing seasons, including sex-ratio, spawning season, spawning location, length at first gonad maturity, gonado-somatic index, and fecundity. Total fish samples (n=1214)were collected monthly from November 2013 to October 2014 at four locations in the Mahakam River, using many fishing gears.The results of this research showthe total length of fish ranged from 132.19 to 227.30 mm and weight ranged from 20.00 to 70.40 g. The overall sex ratio of male and female was1: 1.56, while at gonad maturity stages were1:1.77. The spawning season range from November to January and peak spawning occurs in December. The highest spawning location was found at swamp flood Semayang Lake. The length at first gonad maturity of male ranged of 191.05-202.60 mm, while it was in 179.56-198.50 female. Maximum average gonado-somatic index (GSI) values obtained for male and female were 0.32 and 2.07 respectively during spawning period in November and declined to minimum in February. The total fecundity and eggs diameter rangedfrom 2648 to 12495 eggsind-1and 0.61 to 1.30 mm respectively. There was a positive correlation between fecundity andtotal length and weightof fishes.AbstrakBiologi reproduksi ikan lais O. miostoma (Vaillant 1902)sebagai salah satu spesies endemik di Sungai Mahakam Kalimantan Timur belum pernah diketahui. Tujuan penelitian ini adalah menganalisisaspek reproduksi ikan laisberkaitan dengan perubahan musimyang mencakupnisbah kelamin, musim pemijahan, lokasi pemijahan, ukuran kali pertama matang gonad, indeks kematangan gonad dan fekunditas.Total ikan contoh 1214 ekor telah dikumpulkan setiap bulan mulai dari bulan November 2013 sampai Oktober 2014di empat lokasi perairan Sungai Mahakam, menggunakan berbagai alat tangkap. Hasil penelitian menunjukkanukuran panjang total ikan berkisar dari 132,19-227,30 mm dan bobot berkisar dari 20,00-70,40 gram.Nisbah kelamin seluruh ikan jantan dan betina yang diamati 1 : 1,56, sedangkanpada tahap kematangan gonad 1:1,77. Musim pemijahan terjadi mulai dari bulan November sampai Januari dan puncak pemijahan pada bulan Desember. Lokasi pemijahan tertinggi ditemukan di rawa banjiran Danau Semayang. Ukuran ikan pertamamatang gonad pada jantan berkisar dari 191,05-202,60 mm dan betina berkisar dari 179,56-198,50 mm. Rata-rata indeks kematangan gonad (IKG) tertinggi ditemukan pada jantan dan betina berturut turut 0,32 dan 2,07 selama musim pemijahanpada bulan November dan menurun hingga terendah pada bulan Februari. Fekunditas total berkisar dari 2.648-12.495 butir telur per individu.Ada korelasi positif antara fekunditas dengan panjang total dan bobot ikan.
Ris Dewi Novita, Kukuh Nirmala, Eddy Supriyono, Idil Ardi
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.410

Abstract:Orange clownfish is one of the most desired marine ornamental fish by global market due to the orange color on it. The obstacles of the clownfish farming is the changes of the orange color on clownfish. Manipulation of the light spectrum exposure may affect the amount of chromatophore, that can change the color of clownfish become brighter. The present study was aimed at determining an appropriate spectrum of LED light toward growth and color quality of Amphiprion percula juvenile farming. The study was conducted in five treatments with three replication. The treatment consists of four types of LED light with different wavelengths i.e., white (P), red (M), green (H) and blue (B) with 12 hours and control. Clownfish with an average body weight of 0.91±0.19 g and length of 3.28±0.24 cm were used in this study. The result after 60 days showed that the blue LED light give the best glucose levels of 40.00±2.65 mg dL-1, malondialdehyde levels of 9.30±0.29 nmol mL-1, specific growth rate of 1.71±0.05% and feed efficiency of 78.23±1.97%. The best color quality parameters on blue LED light treatment with the Toca color finder (TCF) score reach the orange color on the dorsal, caudal and anal, RGB ratio on the color of the dorsal, caudal and anal each of 64.59±1.00%, 68.12±0.74% and 72.56±0.20% as well as the number of chromatophore each of 346±10 cells/ 0.1 mm-2. The spectrum blue LED light was able to result the growth and quality of best color for clownfish Amphiprion percula juvenile.AbstrakIkan badut Amphiprion percula merupakan ikan hias air laut yang diminati pasar global ikan hias karena memiliki daya tarik tersendiri pada warna jingga yang dimilikinya. Ikan badut hasil budi daya memiliki kualitas warna jingga yang cenderung memudar. Penggunaan manipulasi spektrum cahaya dalam sistem budi daya dapat memengaruhi perubahan jumlah kromatofor yang dapat meningkatkan warna ikan menjadi terang. Tujuan penelitian ini adalah menentukan spektrum cahaya lampu LED yang tepat terhadap pertumbuhan dan kualitas warna yuwana ikan badut. Penelitian ini menggunakan Rancangan Acak Lengkap pada lima perlakuan dan tiga ulangan. Perlakuan terdiri atas empat jenis spektrum cahaya lampu LED dengan panjang gelombang berbeda yakni putih (P), merah (M), hijau (H) dan biru (B) dengan lama penyinaran 12 jam dan kontrol. Rerata panjang total awal ikan uji adalah 3,28±0,24 cm dan bobot 0,91±0,19 g. Hasil penelitian selama 60 hari pemeliharaan menunjukkan bahwa parameter respons fisiologis dan pertumbuhan terbaik adalah pada perlakuan lampu LED biru dengan kadar glukosa sebesar 40,00±2,65 mg dL-1, kadar malondialdehyde (MDA) sebesar 9,30±0,29 nmol mL-1, laju pertumbuhan spesifik (LPS) sebesar 1,71±0,05% dan efisiensi pakan (EP) sebesar 78,23±1,97%. Parameter kualitas warna terbaik pada perlakuan lampu LED biru dengan skor dalam Toca color finder (TCF) mencapai warna jingga pada bagian dorsal, caudal dan anal, red,green and blue (RGB) ratio pada warna bagian...
Hasan Ayyub, Agung Budiharjo
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.378

Abstract:There are population of silver barb (Barbonymus gonionotus) in Bengawan Solo River, Dengkeng River, Opak River and Gajah Mungkur Reservoir. Bengawan Solo River’s flow meet Dengkeng River’s flow while Opak River’s flow separated from the two other made an interesting phenomenon of population separability to study diversity and grouping between populations through morphological markers. The study aimed to analyze the diversity of silver barb in the Bengawan Solo River, Dengkeng River, Opak River and Gajah Mungkur Reservoir based on morphological characters. Measurement of morphological characters with morphometric methods with 14 measured morphological characters. Measurement of water chemical and physical factors include temperature, current velocity, depth, pH, dissolved oxygen and substrate. The result showed that the four distinguished morphological characteristics of the population predominantly in silver barb (B. gonionotus) were the dorsal depth, snouth length and caudal pundacle depth. Group analysis showed that silver barb population of the Bengawan Solo River had morphological characters more similar to the Dengkeng River than Opak River and Gajah Mungkur Reservoir because of the similarity of habitat and proximity of the location. Based on the most influential environmental factors is the difference in current velocity.AbstrakPopulasi ikan tawes (Barbonymus gonionotus ) terdapat di Sungai Bengawan Solo, Sungai Dengkeng, Sungai Opak, dan Waduk Gajah Mungkur. Aliran Sungai Bengawan Solo bertemu dengan aliran Sungai Dengkeng sedangkan aliran Sungai Opak terpisah dari keduanya. Hal ini menjadikan sebuah fenomena keterpisahan populasi yang menarik untuk diteliti keragaman dan pengelompokan antar populasi melalui penanda morfologis. Penelitian ini bertujuan untuk menganalisis keragaman ikan tawes di Sungai Bengawan Solo, Sungai Dengkeng, Sungai Opak, dan Waduk Gajah Mungkur berdasarkan karakter morfologis. Pengukuran karakter morfologis dengan metode morfometrik dengan 14 karakter morfologis yang diukur. Pengukuran faktor fisika kimia air meliputi suhu, kuat arus, kedalaman, pH, oksigen terlarut, dan substrat. Hasil penelitian menunjukkan karakter morfologis pembeda keempat populasi tersebut yang dominan pada ikan tawes adalah tinggi sirip dorsal, panjang moncong, dan tinggi batang ekor. Analisis kelompok menunjukkan populasi ikan tawes Sungai Bengawan Solo memiliki karakter morfologis lebih mirip dengan Sungai Dengkeng daripada Sungai Opak dan Waduk Gajah Mungkur karena kemiripan habitat dan kedekatan lokasi. Faktor lingkungan yang paling berpengaruh adalah perbedaan kuat arus perairan.
Rosidah, Ibnu Dwi Buwono, Walim Lili, Ibnu Bangkit Suryadi, Ade Reza Triandika
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.435

Abstract:This study aims to reveal effective concentration of moringa leaf extract which is inducing body resistance of sangkuriang catfish against Aeromonas hydrophila. This study used as experimental method of Completely Randomized Design (CRD) with six treatments and three replications. The treatment used in this research are the addition of Moringa leaf extract into feed with concentration of 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) and 200 ppm (F). Observed Parameters are total leukocyte count, increase in leukocyte, clinical sign and survival rate. Increase in leukocyte and survival rate data were analyzed using F test and Duncan test with significance level 0,05, while clinical sign data was analyzed descriptively. The results showed that Moringa leaf extract was effective to induce the immune of sangkuriang catfish. The 150 ppm concentration resulted the highest total leukocyte count of 23.46 ± 6.46% and didn’t have clinical symptons after challenge with Aeromonas hydrophila and highest survival rate 80.0 ± 5.00%. Based on the regression analysis, 153.95 ppm of Moringa leaf extract is the optimum concentration with 95.62% on the survival of sangkuriang catfish after challenge with Aeromonas hydrophila.AbstrakPenelitian ini bertujuan untuk menentukan konsentrasi yang efektif dari ekstrak daun kelor untuk menginduksi ketahanan tubuh ikan lele sangkuriang terhadap serangan bakteri Aeromonas hydrophila. Penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap dengan enam perlakuan dan tiga ulangan. Perlakuan penelitian yang digunakan adalah penambahan ekstrak daun kelor ke dalam pakan dengan konsentrasi 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) dan 200 ppm (F). Parameter yang diamati meliputi jumlah total sel darah, peningkatan jumlah sel darah putih, gejala klinis, dan sintasan. Data peningkatan jumlah sel darah putih dan sintasan dianalisis menggunakan uji F dan uji jarak berganda pada taraf kepercayaan 5%, sedangkan data gejala klinis dianalisis secara deskriptif. Hasil penelitian menunjukkan ekstrak daun kelor efektif untuk menginduksi ketahanan tubuh benih lele sangkuriang. Konsentrasi 150 ppm menghasilkan rata-rata jumlah sel darah putih tertinggi 23,46±6,46% dan setelah diuji tantang tidak menunjukkan gejala klinis, serta tingkat sintasan yang tertinggi sebesar 80,0±5,00%. Berdasarkan analisis regresi konsentrasi ekstrak daun kelor memberikan pengaruh sebesar 95,62% dengan konsentrasi optimum sebesar 153,95 ppm terhadap sintasan ikan lele sangkuriang setelah di uji tantang dengan Aeromonas hydrophila.