AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Journal Information
ISSN / EISSN
:
2615-2207 / 2579-843X
Published by: Universitas Bangka Belitung
(10.33019)
Total articles ≅ 75
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Latest articles in this journal
Published: 30 June 2022
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 6, pp 1-11; https://doi.org/10.33019/agrosainstek.v6i1.192
Abstract:
The development of the Ulap Doyo weaving industry in East Kalimantan is constrained by doyo plants availability as raw material. Cultivation of Doyo or Marasi plant conventionally faces obstacles due to the low germination rate and deteriotation of doyo seeds. Tissue culture is considered as an important technology for mass propagation producing disease-free, high quality, uniform and rapid production of planting material. This study aims to induce regeneration and increase the multiplication of doyo plant for mass multiplication to support the doyo plant conservation program. The research was conducted in 3 stages: shoot initiation, shoot multiplication and plant regeneration (root induction). Shoot initiation and multiplication experiments were carried out using several combinations of 6-Benzylaminopurine (BAP) and Indole-3-butyric acid (IBA) in solid MS media, and two explants types, shoots and rhizomes. The root induction was carried out by growing the plants in MS media containing 0.25 mg L-1 IBA. The results showed that the rhizome was the best part of the plant as a source of explants in doyo plant tissue culture. Initiation medium of 3.75 mg L-1 BAP + 0.50 mg L-1 IBA and the multiplication medium of 37.50 mg L-1 BAP + 0.50 mg L-1 IBA, gave the best effect resulting in the highest survival and responsiveness percentage (80%), percentage of shoot producing explants( 60%, and number of shoots per explant ( 4.00). The plant regeneration has good potential for doyo plant mass propagation, in which 87.50% of the resulting shoots were able to form good root system.
Published: 30 June 2022
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 6, pp 43-52; https://doi.org/10.33019/agrosainstek.v6i1.202
Abstract:
Komoditas bawang merah merupakan komoditas strategis dan memiliki nilai ekonomis tinggi serta tidak dapat disubstitusi dengan komoditas lain. Penelitian dilakukan untuk mengetahui korelasi dan pengaruh langsung dan tidak langsung antara karakter pertumbuhan dan komponen hasil terhadap hasil bawang merah di dataran tinggi. Penelitian dilakukan dari bulan September sampai dengan November 2019 di Lembang (Kab. Bandung Barat), Pacet (Kab. Bandung), dan Samarang, (Kab. Garut). Materi penelitian ini meliputi 12 genotipe bawang merah yaitu klon B1, klon B19, klon B63, klon B72, klon B77, klon B102, klon B222, Trisula, Bali karet, Maja cipanas, Bima brebes dan Sumenep. Rancangan percobaan yang digunakan di setiap lokasi adalah Rancangan Acak Kelompok (RAK),diulang 3 (tiga) kali. Hasil analisis korelasi menunjukkan terdapat korelasi positif sangat nyata antara karakter panjang daun, dan berat basah umbi per rumpun terhadap hasil umbi basah per hektar di semua lokasi pengujian. Karakter panjang daun merupakan karakter yang berkorelasi positif sangat nyata dan memiliki pengaruh langsung positif sangat tinggi terhadap hasil umbi basah per hektar di seluruh lokasi pengujian di dataran tinggi. Seleksi bawang merah untuk meningkatkan hasil umbi basah per hektar di dataran tinggi dapat dilakukan secara langsung melalui karakter pertumbuhan yaitu panjang daun.
Published: 30 June 2022
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 6, pp 12-22; https://doi.org/10.33019/agrosainstek.v6i1.286
Abstract:
Biotic and abiotic stress during cultivation is one of the challenges in increasing upland rice production. Stress can be mild to severe, potentially reducing yield. Knowing the ability of plants to adapt to stressful environments from the start is essential information in the assembly of new high-yielding varieties. This study aims to determine stress in 36 upland rice lines and the adaptability of several upland rice lines to environmental stress. The genetic material used was 36 upland rice lines and two comparison varieties with four replications. The line is planted in Lampung, DI. Yogyakarta and East Java, two locations each. That area has different soil types and elevations. Data were analyzed descriptively and tabulated. In addition, the average scoring of biotic and abiotic stress for each location was calculated. The results showed that biotic stresses found in the plantations were Leaf Blast, Neck Blast, Bacterial Leaf Blight, Brown Spot, Red Striped, Rats, Birds, Rice Leaf Roller, and Stem Borer. Meanwhile, the abiotic stresses found were drought and salinity. From 36 tested lines, it showed that G26 was resistant to biotic stress caused by pests and diseases, G29 was drought-tolerant, and G6 was salinity tolerant.
Published: 31 December 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 100-108; https://doi.org/10.33019/agrosainstek.v5i2.162
Abstract:
Lahan pasca tambang timah termasuk dalam karakteristik lahan marjinal karena memiliki tingkat kandungan hara yang rendah. Upaya pemanfaatan lahan pasca tambang timah pada pertanian yaitu budidaya tanaman seperti sorgum dengan pemberian berbagai dosis pupuk kotoran ayam dan N,P,K. Tujuan dari penelitian ini adalah mengetahui respon pertumbuhan dan produksi sorgum di lahan pasca tambang timah dengan pemberian berbagai dosis pupuk kotoran ayam dan N,P,K. Penelitian ini menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Faktor pertama adalah dosis pupuk kotoran ayam yang terdiri dari 100 %, 50 % dan 25 % dosis acuan. Faktor kedua adalah dosis pupuk N,P,K yang terdiri dari 100 % dan 50 % dosis acuan. Hasil penelitian menunjukkan pupuk kotoran ayam berpengaruh nyata terhadap parameter jumlah daun. Pupuk N,P,K berpengaruh sangat nyata terhadap parameter tinggi tanaman, diameter batang, bobot berangkasan basah, panjang akar, hasil biji per tanaman dan produktivitas. Pupuk N,P,K berpengaruh nyata terhadap parameter total padatan terlarut. Pengurangan dosis pupuk kotoran ayam dari 100 % ke 50 % menunjukkan hasil berbeda tidak nyata terhadap jumlah daun. Pengurangan dosis pupuk N,P,K menunjukkan hasil berbeda nyata terhadap semua parameter. Perlakuan A1K1 (pupuk kotoran ayam 100 % + pupuk N,P,K 100 %) merupakan kombinasi perlakuan terbaik untuk pertumbuhan dan produksi sorgum di lahan pasca tambang timah.
Published: 31 December 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 124-132; https://doi.org/10.33019/agrosainstek.v5i2.211
Abstract:
Central Bangka Regency is one of central orange production in Bangka Belitung Islands Province. One of the important pests of sweet orange plants is fruit flies (Diptera: Tephritidae). This research aimed to study the diversity of fruit flies (Diptera: Tephritidae) on sweet orange plantations in Central Bangka Regency, Bangka Belitung Islands Province. The fruit flies were collected using Lynfield traps which installed in three villages producing sweet oranges in Central Bangka Regency. The types of attractants used to trap male fruit flies are methyl eugenol and cue lure. The fruit flies collected during the study were 4795 individuals, 3 genera, and 14 species. Fruit flies collected from the methyl eugenol were different compared to those collected using lure attractant trap. Most species (13 species) of fruit fly were found in Terentang village, and the rest was same (9 species). Bactrocera carambolae and B. dorsalis were the dominant fruit fly species in the three research locations.
Published: 31 December 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 114-123; https://doi.org/10.33019/agrosainstek.v5i2.238
Abstract:
Several research on foxtail millet starch have been studied, but there is no recommended method for isolation of foxtail millet starch. In this study, isolation of Bencoolen foxtail millet starch, using sodium hidroxyde with 1x, 2x and 3x cycles, hexane-Sodium hydroxide treatment, and destilled water were done. The purpose of this study were to determine isolation method of Bencoolen foxtail millet starch which minimum impurities, pasting and morphological properties. This research was conducted with a randomized complete design with isolation method as a factor. The result of this study shown that method of starch isolation with NaOH 0.3% three cycle, produces the highest of starch and amylose content were 83.15% and 19.40%. The moisture, ash, protein, fat and crude fiber content of foxtail millet starch were 8.48%, 0.47%, 4.45%, 0.09%, and 0.21%, respectively. The gelatinization temperature, peak time, viscosity, and break down viscosity value of this starch were 78 oC, 7.6 minutes, 4228 cp, and 2738 cp, respectively. It has the highest brightness (L*), and polygonal shape of starch granule.
Published: 31 December 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 89-99; https://doi.org/10.33019/agrosainstek.v5i2.247
Abstract:
Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).
Published: 31 December 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 109-113; https://doi.org/10.33019/agrosainstek.v5i2.196
Abstract:
Barangan banana (Musa acuminata L.) is a plant that has the biggest contribution to national fruit production. The increasing demand for the banana requires the availability of seedlings, while conventional cultivation is unable to produce healthy, disease-free seedlings in a short time and large amount. In vitro culture is considered to be able to handle the constraints of the conventional seed supply. The purpose of this study was to determine the effect of foliar fertilizers on in vitro multiplication of barangan bananas. The method used was a single factor of Completely Randomized Design (CRD), the addition of leaf fertilizer consisting of five levels of media treatment, media MS (control), and 0.50 ml L-1 (p1); 0.75 ml L-1 (p2); 1.00 ml L-1 (p3); 1.25 ml L-1 (p4); 1.50 ml L-1 (p5) of leaf fertilizer media repeated five times. The data were analyzed with Analysis Variance then followed by Duncan Multiple Ranged Test of 5%. The results showed MS media still gave the best results compared to other leaf fertilizer media. Meanwhile, the leaf fertilizer media used in this study still gave good results on the initial time of buds appearance (p3 treatment), the number of shoots and the number of leaves in the p5 treatment, as well as the height of the plant in the p4 and p5 treatments. Therefore, the leaf fertilizer can be used to substitute MS media
Published: 30 June 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 81-88; https://doi.org/10.33019/agrosainstek.v5i1.265
Abstract:
Tanaman nilam merupakan tanaman penghasil minyak atsiri dengan nilai ekonomi tertinggi dibandingkan tanaman penghasil minyak atsiri lainnya. Keragaman genetik tanaman nilam tergolong rendah, karena di Indonesia baru terdapat lima klon unggul, dan keterbatasan ini menyebabkan pilihan varietas untuk budidaya juga terbatas. Oleh karena itu perlu dilakukan upaya untuk menghasilkan klon unggul baru yang memenuhi standar nasional. Penelitian ini menguji sembilan genotipe unggul nilam hasil mutasi pada dataran rendah. Tujuan utama penelitian untuk mendapatkan kestabilan hasil, variabilitas yang luas, mengetahui korelasi tiap parameter, dan kedekatan kelas sepuluh genotipe nilam. Penelitian menggunakan bahan tanam sembilan klon tanaman nilam hasil koleksi dan satu varietas nilam yang telah rilis di Indonesia sebagai pembanding, yaitu Lhoksemauwe. Perlakuan disusun dalam Rancangan Kelompok Teracak Sempurna dengan tiga ulangan. Berdasarkan hasil penelitian tidak terdapat perbedaan signifikan pada hampir seluruh parameter pengamatan kecuali pada parameter diameter batang. Genotipe yang digunakan sudah menunjukkan kestabilan hasil pada lokasi Rajabasa Lampung Selatan. Variabilitas pada hampir seluruh parameter menunjukkan kriteria luas kecuali pada parameter turgiditas sel dan rendemen minyak. Korelasi positif yang signifikan terjadi antara beberapa parameter pengamatan. Kelas yang terbentuk sebanyak empat kelas dan yang memiliki kedekatan lebih dari 90% ada tiga kelas.
Published: 30 June 2021
Agrosainstek: Jurnal Ilmu Dan Teknologi Pertanian, Volume 5, pp 72-80; https://doi.org/10.33019/agrosainstek.v5i1.233
Abstract:
Bamboo are known having a high adaptive ability to tolerate environmental changes or stresses. Endogenous microorganisms in several parts of bamboo have been reported used as organic fertilizer and biocompost. However, bacterial potential as auxin (IAA) producer has not been widely report, especially for Torajas’ local bamboo. In this study, rhizosphere and endophytic bacteria were isolated from six different bamboo. Bamboo samples were obtained from the bamboo forest station area of North Toraja. Rhizosphere bacterial isolates were obtained from the area around the roots of bamboo plants, while endophytic bacterial isolates were obtained from roots and shoots bamboo tissue. Six rhizosphere isolates and 12 endophytic isolates were obtaind. All isolates were indentified for morphological, physiological, biochemical tests and IAA’s activities. There are 12 IAA-producing isolates, which where dominated by endophytic bacterial isolates. Based on 16S molecular identification, it was found that K12 isolates were similar to Bacillus cereus, with an IAA concentration value was 1.301 mg L-1. While K14 isolated has similiarities with Stenotrophomonas maltophilia with the abiliy to produce IAA was 2.737 mg L-1. The reconstruction of the phylogeny tree showed that K12 isolate had similiarity with Bacillus wedimannii, and K14 isolate was related to Stenotrophomonas sp.