Jurnal Ilmu Ternak dan Veteriner

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ISSN / EISSN : 08537380 / 2252696X
Total articles ≅ 226
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Nurul Afzan Hilda Zakiya, A H Yanti, T R Setyawati
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 68-73; doi:10.14334/jitv.v25i2.2026

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.
Forough Masoumi, Mehrdad Shariati, Mokhtar Mokhtari
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 91-98; doi:10.14334/jitv.v25i2.2467

As an organophosphorus, Diazinon (DZN) impairs liver tissue function by inhibiting acetylcholinesterase and causing oxidative stress. In this study, the effects of Silybum marianum aqueous extract (SMAE) and L-carnitine (LC) on the stereological and histopathological changes of the liver in DZN-treated male rats were investigated. The rats in this study were placed into 9 groups of 8 each containing control, placebo, and a combination of DZN, SMAE, and LC. The animals received SMAE and chemicals orally for 30 days. At last, the liver tissue of all animals was removed. Then, tissue sections from the liver were provided to study the stereological markers including liver volume and weight, hepatocytes’ volume, central venous volume, sinusoidal volume, connective tissue volume, inflammation rate, and a number of the hepatocytes’ nuclei. Also, the sample tissues were evaluated histopathologically. Treatment with DZN significantly reduced the liver volume and weight, hepatocyte volume, central venous volume, sinusoidal volume, and hepatocyte nucleus number compared to placebo and control but it significantly increased the inflammation and volume of liver’s connective tissue. However, co-administration of SMAE and LC with DZN improved liver volume and weight, hepatocyte volume, central venous volume, sinusoidal volume, connective tissue volume, and hepatocyte nucleus number alone compared to the DZN treatment. Liver inflammation was also significantly decreased compared to the DZN treatment but comparing to the placebo and control groups, it increased significantly. Simultaneous administration of SMAE and LC has protective effects on liver tissue and can reduce DZN-induced liver injury in rats.
Anneke Anggraeni, F Saputra, A Hafid, Abl Ishak
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 48-59; doi:10.14334/jitv.v25i2.2498

Information on non-genetic and genetic factors is required in the selection program. Indonesian Research Institute for Animal Production (IRIAP) has been conducting a selection of the growth traits of Sapera goat (50% Saanen, 50% PE). This research was aimed to study non-genetic and genetic effects on growth traits from birth to the age of 120 days old of the 2nd generation (G2) of Sapera goat. Data on body weight and measurement were collected from kids at birth (105 head.) to the age of 120 days old (51 head). The 30 days interval growth data were calculated by linear interpolation. Non-genetic effects were analyzed by General Linear Model for unbalanced data by considering sex, type of birth, the month of kidding, and year of kidding as fixed variables. The genetic component was analyzed by a mixed linear model by considering sire as a random variable. Heritability was estimated by the paternal half-sib method. Non-genetic factors mostly had no significant effect (P> 0.05) on body weight and measurement. The 90 days old and 120 days old males had higher weights than females (P<0.05). Birth type and year of kidding had significant effects (P<0.05) on body weight and some measurements at certain ages. No significant months of kidding effect on the growth traits (P>0.05). Heritability values of body weight (h2 = 0.11-0.19) and body sizes (h2 = 0.03-0.24) were relatively low. Except high heritability values for birth weight and for body weight at 30 days old (h2 = 0.59 and 0.29), and for hip girth at 30 days old and at 60 days old (h2 = 0.13-0.54). The growth traits of G2 Sapera kids were affected by sex and year of kidding and slightly influenced by genetic (sires) factors.
Omar R Mardenli, Ms Mohammad, Ha Hassooni
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 60-67; doi:10.14334/jitv.v25i2.2459

The use of cryoprotectants in vitrification would reduce the critical damages to the embryos, thus increase the survival rates. This research was conducted in the laboratory of reproductive biotechnology at the faculty of Agriculture of Aleppo University. The study aimed to evaluate the viability and survivability of early Syrian Awassi embryos under the influence of dimethyl sulphoxide (DMSO) and ethylene glycol (EG) following vitrification. Embryos were vitrified in three solutions of cryoprotectants (A: DMSO (3 ml), B: EG (3 ml), and C which was composed of a combination of DMSO (1.5 ml) and EG (1.5 ml)). After thawing, embryos that had been vitrified in C solution achieved the highest rates of cleavage (P< 0.01) comparing with A and B solutions for 2-16 cell stage (50.00% Vs 30.77% and 36.36%), morula (9.00% Vs 44.44% and 40.00%) and blastocyst stage embryos (92.86% Vs 58.33% and 50.00%) respectively. Down to the hatching blastocyst stage, 2-16 cell stage vitrified embryos in C solution achieved an encouraging rate comparing with A and B solutions (39.20% Vs23.08% and 22.73% respectively). The rates of arrested embryos decreased significantly (P< 0.05) after thawing across the three solutions especially the morula and blastocyst stage (0.00 and 3.70% respectively) (C solution). No significant differences were observed in the three types of embryos across all stages and solutions despite the large range among these rates. Given the apparent benefit of the participatory effect of cytoprotectants, it is advised to use a mixture of DMSO and EG (1:1) in vitrification of ovine embryos.
Arnold Parlindungan Sinurat, Tiurma Pasaribu, Tresnawati Purwadaria, Tuti Haryati, Elizabeth Wina, Tri Wardhani
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 81-90; doi:10.14334/jitv.v25i2.2501

Antibiotics (AGP) have been used as feed additives to promote growth and feed efficiency in poultry production. However, many countries include Indonesia now ban the use of AGP and attempts are made to replace the antibiotic to maintain good performances of broilers. Plant bioactives is one of the alternatives that could replace the AGP. An experiment was conducted in an attempt to replace the AGP in broiler feed with a mixture of some plant bioactives (liquid smoke of cashew nutshell, Phyllanthus niruri, and clove leaves). Eight (8) dietary treatments were formulated to have similar nutrients consist of negative control (NC), positive control (NC+AGP), diets supplemented with liquid plant bioactives in 3 levels and diets supplemented with powder plant bioactives in 3 levels. Each diet was fed to 6 replications of 10 birds each from 1 to 35 days old. The performances and the immune response of the broilers due to the treatments were observed. Results showed that the powder plant bioactives could not improve the performance of broilers. None of the feed additives (AGP or plant bioactives) affect the immune systems of the broilers. However, liquid plant bioactives in low dose improved the performance of broilers better than the AGP and therefore is suitable to replace the antibiotic as feed additives in broiler diet.
Zuratih Zuratih, Sps Budhi, Z Bachruddin
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 74-80; doi:10.14334/jitv.v25i2.2066

Methane is one of the gases produced by ruminants during feed fermentation in the rumen. This experiment was aimed to investigate the production of monacolin K in rice bran fermented by Monascus purpureus mold and the influence of the supplementation of fermented rice bran using Monascus purpureus mold on elephant grass basal diet on fermentation products and methane production in an in vitro gas production method. The study consisted of two experiments. The first experiment analysis of monacolin K production in fermented rice bran using Monascus purpureus. Fermentation is done by the addition of Monascus purpureus at levels 0, 4, 8, and 12% (v/w) of substrate (rice bran) with 3 replications. Monacolin K in the substrate was analyzed using HPLC. The second experiment was the evaluation of supplementation of fermented rice bran to elephant grass basal diet using in vitro gas production. The treatment diet evaluated were Pennisetum purpureum (control), Pennisetum purpureum:rice bran (1:1 ratio), and Pennisetum purpureum:rice bran fermented. Each treatment was replicated 3 times. Results from the first experiment shows that rice bran with the highest monacolin K content was in rice bran fermented at 12% by Monascus purpureus. Result from the second experiment showed that supplementation of fermented rice bran to Pennisetum purpureum basal diet did not affect rumen ammonia concentration, VFA, protein microbial production, and dry matter and organic matter digestibility. However, methane production (CH4) was reduced (P<0.05) by 50%, and the protozoal population was decreased (P<0.05) by 80%. It is concluded that supplementation of fermented rice brands containing monacolin K was able to reduce methane production and the protozoa population without affecting feed fermentation.
Peni Wahyu Prihandini, A Primasari, M Luthfi, J Efendy, D Pamungkas
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 39-47; doi:10.14334/jitv.v25i2.2496

Information on the genetic diversity of native and local cattle in Indonesia is vital for the development of breeding and conservation strategies. This study was aimed to assess the genetic diversity and phylogenetic relationship of the Indonesian native (Bali) and local [(Donggala, Madura, Sragen, Galekan, Rambon, dan Peranakan Ongole Grade x Bali (POBA)] cattle populations. Genomic DNA was extracted from blood samples (n= 75). Partial sequences of mtDNA cyt b, 464 bp, were amplified using the polymerase chain reaction technique (forward primer: L14735 and reverse primer: H15149). Thirty-four reference sequences of Bos taurus, Bos indicus, and Bos javanicus were included in the phylogenetic analyses. A total of 55 polymorphic sites and 13 haplotypes were observed in the whole breeds. No variable sites of mtDNA cyt b were observed in Galekan (kept in BCRS) and Rambon cattle. Overall haplotype diversity and nucleotide diversity were 0.515 ± 0.070 and 0.0184 ± 0.0045, respectively. The highest (0.092) and the lowest (0.000) genetic distances were between Bali and Donggala cattle populations and among Galekan (kept in BCRS), Rambon, and POBA cattle populations, respectively. Both mtDNA network and phylogenetic analyses revealed two major maternal lineages (A and B) of the studied population. Most of the sampled individuals (69.33%, present in haplotype H8-H19) were linked to lineage B, which belonged to the same cluster with Bos javanicus. Overall, most of the Indonesian native and local cattle populations had a considerable genetic diversity and shared a common maternal origin with Bos javanicus.
Dela Ria Nesti, Ahmad Baidlowi, Achmad Fauzi, Ida Tjahajati
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 19-25; doi:10.14334/jitv.v25i1.2079

The purpose of this research was to determine the effect of the combination of mix culture bacteria (EM4®:E) and fungi (Trichoderma viridae:TV) on physical quality and in vitro rumen fermentation parameters of peanut hull-based feed supplements. Basal feed was divided into four treatments, which were: P0 (BF); P1 (E:25%+TV:75%); P2 (E:50%+TV:50%),; and P3 (E:75%+TV:25%); and each treatment had three replications. Feeds were fermented facultative anaerobically for nine days. The observed parameters were physical qualities (color, odor, fungi appearance and pH), and in vitro rumen fermentation parameters (rumen pH, microbial protein content, and total volatile fatty acid content). Data were analyzed using one way ANOVA design, and the significance of differences were tested using Kruskall-Wallis test for the physical qualities data and Duncan’s New Multiple Range Test (DMRT) test for in vitro rumen fermentation parameters. Results showed that the combination of E and TV at different level did not affect odor and the fungi appearance parameters (P≥0.05), however, it significantly affected P≤0.05) color change from blackish (1.64) in P0 to brownish in P1, P2 and P3 of 2.44; 2.69; and 2.80, respectively. The pH also decreased significantly (P≤0.05) by 10.67%. Treatment also did not affect the rumen pH, microbial protein content, and total volatile fatty acid content (P≥0.05). It is concluded that the combination of the EM4® 25%: Trichoderma viridae 75% on peanut-hull based feed supplement fermentation gives the best result on color and pH fermented feed product without affecting the rumen fermentation process.
Marziyeh Naimi, Mehrdad Shariati, Sirous Naeimi, Mohammad Amin Edalatmanesh
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 11-18; doi:10.14334/jitv.v25i1.2161

Acrylamide (AA) is a toxic and carcinogenic compound produced in cooking process. The purpose of this study is to evaluate the effect of N-acetylcysteine (NAC) on extracellular signal-regulated kinase (ERK) gene expression level and ovarian histopathological changes in AA-treated rats. Thirty-six female adult Wistar rats were randomly divided into 6 groups including control, positive control (+VE Con), negative control (-VE Con), experimental 1 (Exp1), experimental 2 (Exp2) and experimental 3 (Exp3). Twenty eight days after the treatment, ERK gene expression level was measured by real-time PCR method and ovarian histopathological changes were evaluated. The ERK gene expression level was significantly decreased in the +VE Con, Exp1 and Exp2 groups as compared to the control group (p˂0.05), but not in the -VE Con and Exp3 groups (p˃0.05). Histologically, the +VE Con group showed a significant decrease in the number of primary, secondary and Graafian follicles as well as corpus luteum as compared to the control group (p˂0.05), but not in the negative, Exp2 and Exp3 groups (p˃0.05). In the Exp1 group, the number of primary and secondary follicles as well as corpus luteum significantly decreased (p˂0.05), however, the numbers of Graafian follicle and the corpus luteum were significantly increased as compared to the +VE Con group (p˂0.05). The AA was supposed to increase the apoptosis and folliculogenesis degradation in the rat ovarian tissue by decreasing ERK gene expression. Administration of NAC ameliorated the deleterious effects of AA in a dose-dependent manner and improve folliculogenesis by reducing apoptosis level. Thus, the NAC supplement could be helpful in ameliorating animal fertility.
David Kurniawan, Citra Dewi Yulia Chistie
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 34-38; doi:10.14334/jitv.v25i1.2053

This study was aimed to investigate performance and quality of broiler duck carcasses fed with Morinda citrifolia and Arthrospira plattensis. A total of 168 two-week-old broiler duck with an initial average body weight of 463 ± 29.38 g and a diversity of 6.35 % were randomly allotted to 7 experimental groups with 4 replications each with 6 bird per replication. Treatments were T0 (basal diet as a control), T1 (basal diet + 0.2% of Morinda citrifolia powder (MP)), T2 (basal diet + 0.5% of Arthrospira plattensis powde(AP)) , T3 (basal diet + 0.2% of MP+ 0.5% of AP), T4 (basal diet + 0.4% of MP + 0.5% of AP), T5 (basal diet + 0.2% of MP + 0.1% of AP), T6 (basal diet 0.4% of MP + 0.1% of AP). Variables measured were feed intake, body weight gain, feed conversion ratio, carcass percentage, abdominal fat, and visceral organ. Data were analyzed for variance based on a Completely Randomize Design and continued with Duncan’s multiple Range Test for differences. Result showed that the treatments did not affect (P>0.05) on feed intake, body weight gain and feed conversion ratio. The treatments also did not affect (P>0.05) carcass percentage, abdominal fat, and visceral organ. The diet did not significantly improve performance and quality of broiler duck carcasses.
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