Jurnal Ilmu Ternak dan Veteriner

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ISSN / EISSN : 08537380 / 2252696X
Total articles ≅ 196
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Hendry Tri Saragih, F. Viniwidihastuti, R. P. Lembayu, A. R. Kinanthi, H. Kurnianto, I. Lesmana
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1889

Abstract:The needs for chicken meat have been dominated by meat from exotic broilers (bred from imported parent). The potential of local broilers chicken in Indonesia is expected to be able to provide the meat for national needs for chicken meat. The objective of this research was to determine the day-old-chick (DOC) phenotypic characteristics of chickens in Indonesia that have the potential as broilers. The phenotypic characteristics are in the form of body morphometry, visceral organ weight and small intestinal histo-morphology. The chickens used were the DOC type of exotic Broiler, Kampung, exotic male Layer, KUB-1 and Pelung. A total of 125 chickens consisting of 25 chickens of each strain at posthatched age were observed for its body morphometry and its visceral organ weight, and small intestinal histo-morphology. The observed data were then analyzed by similarity analysis using MVSP 3.22 to create a dendogram with the Unweighted Pair Group with Aritmetic Average (UPGMA) method. Results showed that there were 2 different clusters from the level of similarity in their characteristics. Exotic broilers had 94.65% similarity to male exotic layer chicken and 92.26% to pelung chicken, while Kampung chickens had 90.16% similarity to KUB-1Chicken. In conclusion, it is indicated that the level of similarity of the phenotypic characteristics of pelung chickens were close to the type of exotic broiler and male exotic layer chicken. This level of similarity lead to the expectation that pelung chickens potential to be a candidate for meat-type of local chicken.
Cecep Hidayat, Sofjan Iskandar
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1913

Abstract:Sensi-1 Agrinak is a strain of the improved native chickens for meat production in Indonesia. The objective of this study was to investigate influence of different dietary energy and protein levels on performance, meat bone ratio, and meat chemical composition of Sensi-1 Agrinak chicken, reared until 10 weeks of age. Two hundred and sixteen of unsexed day old chickens (DOC) of Sensi-1 Agrinak were subjected to six experimental rations differed in dietary crude protein (CP) content,. Namely: 21;19; and 17 % and dietary metabolizable energy (ME) (2800 and 3000 kcal/kg). Each treatment combination was replicated four times and fed from day old to 10 weeks old. In each treatment combination there were nine unsexed-DOCs. The parameters observed were performance (i.e. live weight, feed intake, viability, FCR), economic index (European Production Efficiency Factor/EPEF), meat bone ratio, and meat chemical composition. Result showed that increased of dietary CP level increased live weight and EPEF (p<0.05). Sensi-1 Agrinak chicken had the best FCR (2.59), when fed diet containing 21% CP and 3000 kcal/kg. Increased dietary CP level increased the meat-bone ratio of breast, thighs, and drumsticks. Meanwhile, increased levels of dietary CP and ME did not affect (p>0.05) meat chemical composition. It is concluded that optimal dietary levels of crude protein and energy for unsexed Sensi-1 Agrinak chicken up to 10 weeks of age were 21% CP and 3000 kcal/kg.
Syahruddin Said, Setiorini Setiorini, Amaitshaa Adella, Indah Sari, Nursafira Fathaniah, Tulus Maulana
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1873

Abstract:The objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on quality of SO bull spermatozoa during freezing and thawing. In this study the DNA stability of frozen-thawed Sperm was also indentified. Three mature bulls maintained at PT. Karya Anugerah Rumpin, private cattle breeding company, West Java, Indonesia were used as semen donors. Semen was collected using artificial vagina and were evaluated prior to freezing. Semen was diluted with TCFY supplemented with different concentrations of amino acids (5, 15 and 25 mM glycine and glutamine, and 3, 5 and 7 mM cysteine) then processed for colling and freezing. Semen quality parameters (subjective motility, viability and membrane and DNA integrity). Data showed that in general the effect of addition of selected amino acids (glycine, glutamine and cysteine) into TCFY extenders on motility, viability and membrane integrity of SO spermatozoa after cooling were significantly different (p<0.05) higher than that of control. Addition of 15 mM glycine, 15 mM glutamine and 5 mM cysteine resulted in significant (p<0.05) increase post-thawing sperm motility and sperm viability as compared to that of control. Furthermore, when spermatozoa were stained with acridine orange after fixation with acetic alcohol, the DNA integrity of post-thawing spermatozoa showed that all spermatozoa were remain intact. In conclusion ,addition of 15 mM glycine, glutamine and 5 mM cysteine increase the cryoprotecting efficacy of bovine bull cryopreservation extender, and furthermore all DNA spermatozoa were remain intact.
Simson Tarigan, Sumarningsih
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1871

Abstract:The need for rapid diagnostic tools or point- of- care diagnostic tests for Avian Influenza in Indonesia is very high and the price of these imported diagnostic tools is very expensive. As a result, a large budget requires to provide the needs. The main component of a rapid diagnostic tool is the monoclonal antibody (mAb) specifically recognized influenza viruses. The objective of this study was to produce mAb that can recognize all subtypes of Avian Influenza viruses using the phage display technology. Influenza-A focused scFv commercial library was panned using alternating recombinant H1N1 NP and H5N1 virions. Whereas, bacteriophages bound to the panning baits were eluted with serum from H5N1-infected chickens. Phagemid from suppressor E. coli (TG1) infected with bacteriophage displaying anti-NP on its surface was isolated and then transformed into a non-suppressor E. coli (HB2151) to express NP-scFv. Monoclonal NP-scFv antibody with a molecular weight of about 27 kDa was purified from the culture supernatant using a nickel-chromatography column. The amount of pure NP-scFv obtained was around 1.2 mg /L culture. As an additional component for its use in immunoassays, antibody to NP-scFv was produced in rabbits. The generating polyclonal antibody recognized the NP-scFv specifically and sensitively. The anti-NP-scFv monoclonal antibody and the anti rabbit scFv polyclonal antibody produced in this study are envisaged appropriate for the development of diagnostic tools for point-of-care for Avian Influenza.
Rita S. Dewi, April H. Wardhana, Retno D. Soejoedono, Sri Mulatsih
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1864

Abstract:Surra is a disease attacking livestock caused by a flagellated protozoan, Trypanosoma evansi. Indonesia archipelago is reported as an endemic country of the disease, except Sumba Island. However, Surra outbreak occurred in this Island in 2010 due to livestock movement from the neighbour island, Sumbawa. It generated high mortality in livestock, particularly in horses and buffaloes. The aim of the study was to evaluate the effectiveness of Surra treatment strategies in East Sumba District from 2010-2016 and to estimate the incidence of Surra in the next few months (forecast). The treatment strategy of Surra in East Sumba was divided into 2 (two) periods e.g. the first period in 2010-2011 using Isomethamedium as the single drug (period I) and the second period in 2012 - 2016 using a combination between diminazene aceturate as curative and isomethamedium as a prophylactic drug (period II). All data in the present study was obtained from the local livestock agency of East Sumba District from 2010 – 2016 when Surra outbreak occurred. The effectiveness of those two treatment strategies was compared using the proportion test. The results demonstrated that morbidity and mortality of horses and buffaloes were significantly greater in the period I (2010-2011) compared to period II (2012-2016). The treatment strategy in the period II was able to decrease the proportion of morbidity in horses and buffaloes for 1.44% and 0.66%, respectively. Likewise, the proportion of mortality in period II was also less than the period I from 3.79% to 1.30% for horses and from 2.80% to 0.55% for buffaloes. Based on forecasting study analysis using the control program projected with decomposition method for the next 12 months demonstrated that the treatment strategy in the period II could reduce the incidence and death of livestock by Surra. The treatment strategy using a combination between isometamedium and diminazene aceturate in East Sumba District might be more effective compared to using isometamedium alone.
Rijanto Hutasoit, Riyadi, Juniar Sirait
Jurnal Ilmu Ternak dan Veteriner, Volume 24; doi:10.14334/jitv.v24i1.1923

Abstract:Indigofera zollingeriana (Indigofera) plant is potential feed ingredients. The propagation of this plant is through seed. The low quality of seed is a problem in its development. This study was aimed to evaluate the relationship of pod colour with quality of Indigofera seeds. The study was designed in a complete randomized design consisting of four pod colours and four replications, namely: P1= green, P2= brownish green, P3= brown, and P4= black. The parameters observed were: characteristic and morphology of pods and seeds of Indigofera, the growth of sprouts, and the growth of fungus on Indigofera seed. Results showed that the number of pests was fewest found in P2, brownish green pod (14%). The highest number of seeds was in P1, green pod (5173) and P2, brownish green pod (4944). The highest germination (62%) was detected in P2 (brownish green). The heaviest sprout was in P2, in brownish green pod (0.035g), highest sprout (2.68 cm) in P4, black pod colour. Based on fungus observation, the black pod (P4) provided the fewest result (6.63%), however most fungus grew very well in P1, the green pod (47.88%). It could be concluded that the brownish green pod colour was the best phase for harvesting good quality I. zolligeriana seed.
Endang Tri Margawati, Slamet Diah Volkandari, Indriawati Indriawati, Muhamad Ridwan
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 168-173; doi:10.14334/jitv.v23i4.1915

Abstract:Bali cattle is one of local beef cattle in Indonesia, up to present its performance indicated an inbreeding occurrence. This study was aimed to analyze the genetic diversity and relationship among Bali cattle from several locations in Indonesia based on ETH10 microsatellite marker. Ninety-four (94) DNA samples (89 Bali cattle; 5 Banteng) were analyzed. The Bali cattle samples were from 6 locations in Indonesia (15 Pulukan; 15 Nusa Penida; 14 Bima West Nusa Tenggara/WNT; 10 Mataram, WNT; 20 Riau; 15 South Borneo). DNA Banteng samples were collected from Prigen Malang of East Java. Microsatellite marker of ETH10 labelled HEX was used for amplification. Alleles were analyzed by using Cervus 3.0.7 and GenAlex 6.5. Result showed that there were five (5) alleles found in ETH10 marker i.e., 209; 213; 215; 217; and 219 bp. Average of observed (Ho) and expected (He) heterozygosity were 0.46±0.05 and 0.60±0.03, respectively. Five (5) out of 6 locations were in breeding occurrence except Bali cattle from Mataram was not inbreeding. The longest genetic relationship was between Bali cattle from Mataram and Riau whereas the closest distance was Bali cattle from South Borneo with Mataram. Banteng was closest to Bali cattle from Nusa Penida and the longest was to Bali cattle from South Borneo. This finding indicates there is inbreeding in Bali cattle, therefore it needs to be concerned in bull rotation and semen distribution for increasing the Bali cattle performance.
Harapin Hafid, A Napirah, Sm Sarifu, Rahman, Inderawati, Nuraini, Hasnudi
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 202-209; doi:10.14334/jitv.v23i4.1914

Abstract:This research was aimed to study the effect of electrical stimulation period on physical and organoleptic properties of Muscovy duck meat. This research used 20 female Muscovy ducks, 1.5-2 years of age. The ducks were divided into 5 groups treatments for 4 replications. The treatments were period of electrical stimulation: 0, 5, 10, 15, and 20 minutes. The result showed that period of electrical stimulation did not affect (P>0.05) cooking loss but significantly affected (P<0.05) the tenderness, color, flavour, aroma, pH, and juiciness of duck meat. The best treatment was 20 minutes stimulation.
A. A. Affan, Fma Amirul, Aaa Ghani, S Annas, M Zamri-Saad, Ha Hassim
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 180-188; doi:10.14334/jitv.v23i4.1922

Abstract:Pregnancy ketosis has been recognized as one of the common metabolic disease affecting goat’s meat and milk production. For the present study, sixteen (n=16) individuals of pregnant does at day 80 of pregnancy had been used. A total of 8 does were categorized as control group (healthy pregnant goats), were fed on Napier grass and goat concentrate with water ad libitum, and another 8 does were considered as treatment group which categorized as ketosis based on the clinical signs and presence of ketone body in urine. Blood sample were collected from all goats for biochemical profiles analysis which were glucose, Beta-hydroxybutyrate (BHBA), free fatty acid (FFA), calcium, electrolytes (sodium, potassium, chloride), liver enzyme and hormonal levels (cortisol and insulin). Three does from each group were slaughtered and liver samples were collected for fatty acid profiles study. In this study, the BHBA, FFA, calcium, amino aspartate transferase (AST), gamma glutamyl transferase (GGT) and cortisol hormone were significantly higher in pregnancy ketosis goats as compared to control group. Meanwhile, the concentration of glucose, sodium, potassium, chloride and insulin hormones were lower in pregnancy ketosis goats as compared to control. Furthermore, the fatty acid composition in blood plasma of pregnant goat with ketosis showed higher level of palmitic, stearic and oleic acid, while in liver, palmitic, oleic and linoleic acid was found higher.
Eslam Faid-Allah
Jurnal Ilmu Ternak dan Veteriner, Volume 23, pp 159-167; doi:10.14334/jitv.v23i4.1845

Abstract:This study was carried out to evaluate the sires and dams genetically for milk production and mastitis traits in Egyptian 12 herds of Holstein cattle using Best Linear Unbiased Prediction via MTDFREML program. The data was obtained from a commercial farm called Dena, located in Cairo-Alex Desert Road (80 Km), Menofia, Egypt. Data included 4791 cows, 4227 dams and 248 sires that represented the period from 2007 to 2014. Estimating breeding values for milk production traits as cumulative milk yield at 90 days (90-DM), cumulative milk yield at 180 days (180-DM), cumulative milk yield at 270 days (270-DM), cumulative milk yield at 305 days (305-DM), and number of mastitis infection around the season of lactation (MAST). The averages of the 90-DM, 180-DM, 270-DM, 305-DM and MAST were 3026.3±655.1 kg, 5873.3±1081.1 kg, 7891.1±2692.2 kg, 9611.2±1897.9 kg, and 0.712±1.2 time/parity, respectively. Estimates of heritability for the previous traits were 0.11±0.016, 0.15±0.014, 0.18±0.012, 0.22±0.015, and 0.09±0.029, respectively; genetic variance were 47206.2 kg, 175300.6 kg, 1304654.4 kg, 792411.6 kg and 0.12 time/parity, respectively; and phenotypic variance were 429147.6 kg, 1168670.6 kg, 7248079.9 kg, 3601870.9 kg, and 1.35 time/parity, respectively. The EBV values as average, SD, (Min: Max) for sires were 0.0±0.179 (-0.4: 0.66) for MAST, 0.0±86.176 (-263.1: 245.4) for 90-DM, 0.0±227.523 (-600.3: 800.3) for 180-DM, 0.0±413.48 (-323.3: 1277.7) for 270-DM and 0.0±440.26 (-1280.9: 1565.1) for 305-DM. Also, The EBVs for dams were 0.0±0.055 (-0.14: 0.45) for MAST, 0.033±26.24 (-142.8: 103.0) for 90-DM, 0.074±76.81 (-360.2: 289.6) for 180-DM, -0.045±139.66 (-591.9: 529.2) for 270-DM and 0.266±154.1 (-666.3: 617.6) for 305-DM. These results provide that the selection of sires and dams will improve the traits of milk production and mastitis in this herd because of the wide differences in genetic potential among sires and dams.