Jurnal Ilmu Ternak dan Veteriner

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ISSN / EISSN : 0853-7380 / 2252-696X
Total articles ≅ 239
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Lailatul Rohmah, Sri Darwati, Niken Ulupi, Isyana Khaerunnisa, Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 173-181; doi:10.14334/jitv.v25i4.2525

Abstract:
The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.
Gunawan Sitanggang, Raden Iis Arifiantini, J Jakaria
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 147-152; doi:10.14334/jitv.v25i4.2526

Abstract:
The objective of this study was to evaluate effect of genetic and non-genetic factors on semen characteristics including ejaculate volume, sperm concentration, total sperm number and sperm moetility of Bali cattle. Semen data were collected from the National Centre of Artificial Insemination at Singosari, Malang, East Java, Indonesia. A total of 3,847 ejaculates of 17 Bali bulls from 2014 to 2016 were collected and evaluated. Data were analyzed by restricted maximum likelihood (REML) method using mixed models which the bull was a random effect, while age of bull, season of collection, frequency of ejaculation and collection intervals were the fixed effects. Results showed that age significantly affected all semen characteristics (P<0.01). Season affected only on sperm motility (P<0.01). Effect of frequency of ejaculation and collection intervals was significant on all studied variables (P<0.01), except sperm motility. Repeatability of ejaculate volume, sperm concentration, total sperm number and sperm motility was 0.43; 0.35; 0.32 and 0.31, respectively. It is concluded that age, frequency of ejaculation and collection intervals were the most factors affected semen characteristics of Bali cattle. Repeatability estimations of semen characteristics were moderate to high.
Agus Hadi Prayitno, T H Rahman
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 191-195; doi:10.14334/jitv.v25i4.2514

Abstract:
This study aimed to determine the effect of edamame flour filler substitution on the chemical quality of culled duck meatballs. The research material consisted of culled duck meat, tapioca flour, edamame flour, albumen, garlic, onion, salt, pepper, monosodium glutamate, sodium tripolyphosphate, and ice. The edamame flour filler substitution treatments were P0 (0%), P1 (5%), P2 (10%), P3 (15%), and P4 (20%) from total filler. Each treatment consisted of five replications. The parameters which tested were moisture, protein, fat, fiber, and ash contents. Data on chemical quality results were analyzed by analysis of variance using completely randomized design and if there was significantly different (P<0.01), then it’ll be further tested by the Duncan's New Multiple Range Test. Results showed that the substitution of edamame flour filler starting from the level of 5% was significantly affected water content, protein, fat, fiber, and ash of culled duck meatballs. Edamame flour can be used as a filler substitution up to 20% level by giving a good effect on increasing the meatball protein content, but also having a negative impact with increasing the fat content of culled duck meatballs.
, M Rodiallah, T Astuti, Elfawati Elfawati
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 162-172; doi:10.14334/jitv.v25i4.2541

Abstract:
The purpose of this study was to determine reproductive efficiency, population dynamics, natural increase and estimated output of the Kuantan cattle in the Kuansing Regency, Province of Riau. A total of 311 Kuantan cattle and 99 Kuantan cattle farmers were used in this study through a survey study. Respondent samples were taken from seven districts. Data sampling using purposive sampling with survey methods. Data collection was carried out by interviewing farmers and observing and was analyzed descriptively. Parameters measured were reproductive efficiency, natural increase, estimated output and population dynamics of Kuantan cattle. Results showed that the reproductive efficiency of Kuantan cattle was 1.04%, natural increase 5.14%, the balance of male and female 1: 5, the value of male NRR 50% and female NRR 100.56%, total cattle out 18.69% and total incoming cattle 18.69%, output value 48.88% and estimated population dynamics 2.85%. In conclusion, Kuantan cattle reproduction has not been efficient with the natural increase of the Kuantan cattle was very low, and the replacement stock availability for male and female cattle has not been fulfilled. It is recommended not to release Kuantan cattle in the next 5 years to maintain population balance.
Cahya Setya Utama, Zuprizal Zuprizal, Chusnul Hanim, Wihandoyo Wihandoyo
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 196-205; doi:10.14334/jitv.v25i4.2499

Abstract:
This research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre) , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60) . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old.
Masume Ghorbani Vahed, Ramazan Khanbabaee, Mehrdad Shariati, Mohammad Amin Edalatmanesh
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 153-161; doi:10.14334/jitv.v25i4.2555

Abstract:
This research was conducted in order to determine the effects of omega-3 on oocyte in vitro maturation and the level of expression of tribbles (TRIB1, TRIB2 and TRIB3 genes) in cumulus cells. Eight-ten weeks old NMRI mice were super-ovulated using 7.5 IU pregnant mare’s serum gonadotropin (PMSG, Intraperitoneal) and they were killed after 44 hours and their ovaries were removed. The oocytes were used for in vitro maturation and the cumulus-oocyte complexes (COCs) were released. Cumulus cells and oocytes were assigned into control, ethanol-treated and groups exposed to 10 and 100 μg/ml of omega-3. The cells were prepared to assess the maturation stage in order to evaluate the gene expression level. The data were statistically analyzed. Exposing oocytes to low dose (10 μg/ml) and high dose (100 μg/ml) of omega-3 resulted in a reduced rate of GV-stage oocytes, decreased MI-oocytes and increased MII-oocytes. The enhanced maturity of COCs was also detected in response to a high dose of omega-3 (100 μg/ml). Exposure of cumulus cells to omega-3 (10 and 100 μg/ml) induced TRIB2 and inhibited TRIB3 gene expression level; however, TRIB1 gene expression level increased and decreased in response to low (10 μg/ml) and high (100 μg/ml) concentrations of omega-3, respectively. The addition of omega-3 to the environment of oocytes or cumulus cells affected the maturation of oocytes and cumulus cells, which was followed by the differential expression of TRIB genes, suggesting that there was a role of fatty acid metabolism in the differentiation and maturation of cumulus cells.
Fitra Aji Pamungkas, Bagus Priyo Purwanto, Wasmen Manalu, Ahmad Yani, Riasari Gail Sianturi
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 120-130; doi:10.14334/jitv.v25i3.2522

Abstract:
Infrared thermography (IRT) is an alternative solution that can be applied to replace invasive methods currently used in the monitoring of goats' physiological and hematological parameters. This study was done to compare and correlate the physiological and hematological conditions of young Sapera dairy goats and their correlations with results obtained by IRT. Four young Sapera dairy goats (weight of 26-28 kg) were kept in the individual rearing cage. Skin surface temperature (TS), rectal temperature (TR), body temperature (TB), heartbeat (HR), respiration rate (RR), and IRT at eyes, mouth, nose, legs, left body, right body, vagina, and vulva were monitored from 6 a.m. to 6 p.m. in 2 h intervals. Blood samplings were done at the beginning and the end of the obsevation time. Results showed that IRTs at several body parts were positively correlated with physiological parameters, except for heartbeat. Negatively correlation was observed in hematological parameters. The highest correlation (r = +0.85) was observed in the correlation between the results of the left rear leg IRT on TB. It was concluded that IRT can be applied to examine goats’ physiological conditions especially body temperature.
Andi Saenab, Komang G Wiryawan, Y Retnani, Elizabeth Wina
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 139-146; doi:10.14334/jitv.v25i3.2475

Abstract:
The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 treatments and 4 replications. A series of biofat (BF) and biochar (BC) combination were added each to substrate as followed BFBC1 = 0: 100%; BFBC2 = 25:75%; BFBC3 = 50:50%; BFBC4 = 75:25%; BFBC5 = 100: 0%. While, a series of biofat (BF) and biosmoke (BS) combination as followed BFBS1 = 0: 100%; BFBS2 = 25:75%; BFBS3 = 50:50%; BFBS4 = 75:25%; BFBS5 = 100: 0%. Both series used a control treatment which contained only substrate. The in vitro experiment was repeated 4 times and each treatment was done in duplicates. The measured variables were: total gas and CH4 productions, dry matter, organic matter, NDF degradability, NH3 and partial VFA concentrations. The results showed that the combination of biofat and biochar levels resulted in a significant decrease (P
Santiananda Arta Asmarasari, Cece Sumantri, A Gunawan, E Taufik, Anneke Anggraeni
Jurnal Ilmu Ternak dan Veteriner, Volume 25, pp 99-111; doi:10.14334/jitv.v25i3.2502

Abstract:
Protein content in milk is an important indicator of milk. Accordingly, genetic improvement to produce Holstein Friesian (HF) dairy cattle is important. The objective of this study was to evaluate the genetic variant of milk protein genes and its effect on milk component traits of Holstein Friesian (HF). A total of 100 HF were used in this study. The HF cattle used have physiological status in the lactation period 1 up to 3 and lactation change of 1 up to 12 months. Genotype variants of milk protein genes were identified using Real Time-Polymerase Chain Reaction method. Analysis of milk component was carried out covering the component of protein, fat, lactose, and solid non-fat (SNF) by using a milk quality measuring device (Lactoscan). Genotyping of cattle blood samples consisted of DNA extraction, genes amplification using the RT-PCR method. The result showed that protein milk was significantly affected (p<0.05) by the genetic variants of CSN1S1-192 and CSN2-67 genes. Fat milk was significantly affected (p<0.05) by the genetic variants of CSN1S1-192 and CSN3 genes. Meanwhile, solid non-fat milk was significantly affected (p<0.05) by the genetic variants of CSN-BMC9215, CSN-BMC6334, CSN1S1-14618, CSN2_67, and CSN3 genes. Lactose milk was significantly affected (p<0.05) by the genetic variants of CSN-BMC9215 and CSN2-67 genes. It was concluded that genetic variants of the milk protein genes have an association with the component of cow's milk (protein, fat, solid non-fat, and lactose).
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