International Journal of Bioprinting

Journal Information
ISSN / EISSN : 24247723 / 24248002
Current Publisher: Whioce Publishing Pte Ltd (10.18063)
Total articles ≅ 88
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Krishna C. R. Kolan, Julie A. Semon, Bradley Bromet, Delbert E. Day, Ming C. Leu
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.2.204

Abstract:Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12 × 12 × 1 mm3 in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alg-Gel composite hydrogel is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate the feasibility of the solvent-based bioprinting process to fabricate 3D cellularized scaffolds with more than 80% viability on day 0. The decrease in viability after 7 days due to glass concentration and static culture conditions is discussed. The feasibility of modifying Alg-Gel with 13-93B3 glass for bioprinting is also investigated, and the results are discussed.
Sanjairaj VijayaVenkataRaman, Novelia Vialli, Jerry Y.H. Fuh, Wen Feng Lu
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.1.229

Abstract:Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing and other biomedical applications. The success of this technology lies with development of suitable bioinks and hydrogels that are specific to the intended tissue application. For applications such as neural tissue engineering, conductivity plays an important role in determining the neural differentiation and neural tissue regeneration. Although several conductive hydrogels based on metal nanoparticles such as gold and silver, carbon-based materials such as graphene and carbon nanotubes (CNTs) and conducting polymers such as polypyrrole (PPy), and polyaniline (PANi) were used, they possess several disadvantages. The long-term cytotoxicity of metal nanoparticles and carbon-based materials restricts their use in regenerative medicine. The conductive polymers on the other hand are non-biodegradable and possess weak mechanical properties limiting their printability into 3D constructs. The aim of this study is to develop a biodegradable, conductive and printable hydrogel based on collagen and a block copolymer of PPy and Polycaprolactone (PCL) (PPy-b-PCL) for bioprinting of neural tissue constructs. The printability including the influence of the printing speed and material flowrate on the printed fiber width, rheological properties and cytotoxicity of these hydrogels were studied. The results prove that the collagen/PPy-b-PCL hydrogels possessed better printability and biocompatibility. Thus, the collagen/PPy-b-PCL hydrogels reported in this study has the potential to be used in the bioprinting of neural tissue constructs for repair of damaged neural tissues and for drug testing or precision medicine applications.
Yi-Wen Chan, Hsin-Yuan Fang, Ming-You Shie, Yu Fang Shen
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.197

Abstract:With the development of three-dimensional (3D) printing, many commercial 3D printing materials have been appliedin the fields of biomedicine and medical. MED610 is a clear, biocompatible PolyJet material that is medically certified forbodily contact. In this study, the polydopamine (PDA)/hydroxyapatite (HA) coating was added to the printed MED610 objectsto evaluate its physical properties, cell proliferation, cell morphology, and alkaline phosphatase expression level. The resultsshow that the PDA/HA coating helps printed objects to enhance the hardness, biocompatibility, and osteogenic differentiationpotential. We expect that PDA/HA coatings contribute to the applicability of MED610 in biomedical and medical applications
Ming-You Shie, Hsin-Yuan Fang, Yen-Hong Lin, Alvin Kai-Xing Lee, Joyce Yu, Yi-Wen Chen
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.210

Abstract:In recent years, the additive manufacture was popularly used in tissue engineering, as the various technologies for this field of research can be used. The most common method is extrusion, which is commonly used in many bioprinting applications, such as skin. In this study, we combined the two printing techniques; first, we use the extrusion technology to form the ceramic scaffold. Then, the stem cells were printed directly on the surface of the ceramic scaffold through a piezoelectric nozzle. We also evaluated the effects of polydopamine (PDA)-coated ceramic scaffolds for cell attachment after printing on the surface of the scaffold. In addition, we used fluorescein isothiocyanate to simulate the cell adhered on the scaffold surface after ejected by a piezoelectric nozzle. Finally, the attachment, growth, and differentiation behaviors of stem cell after printing on calcium silicate/polycaprolactone (CS/PCL) and PDACS/PCL surfaces were also evaluated. The PDACS/PCL scaffold is more hydrophilic than the original CS/PCL scaffold that provided for better cellular adhesion and proliferation. Moreover, the cell printing technology using the piezoelectric nozzle, the different cells can be accurately printed on the surface of the scaffold that provided and analyzed more information of the interaction between different cells on the material. We believe that this method may serve as a useful and effective approach for the regeneration of defective complex hard tissues in deep bone structures.
Yi Zhang
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.192

Abstract:As microfluidic devices are designed to tackle more intricate tasks, the architecture of microfluidic devicesbecomes more complex, and more sophisticated fabrication techniques are in demand. Therefore, it is sensible to fabricatemicrofluidic devices by three-dimensional (3D)-printing, which is well-recognized for its unique ability to monolithicallyfabricate complex structures using a near-net-shape additive manufacturing process. Many 3D-printed microfluidic platformshave been demonstrated but can 3D-printed microfluidics meet the demanding requirements in today’s context, and hasmicrofluidics truly benefited from 3D-printing? In contrast to 3D-printed microfluidics, some go the other way around andexploit microfluidics for 3D-printing. Many innovative printing strategies have been made possible with microfluidicsenabled3D-printing, although the limitations are also largely evident. In this perspective article, we take a look at the currentdevelopment in 3D-printed microfluidics and microfluidics-enabled 3D printing with a strong focus on the limitations of thetwo technologies. More importantly, we attempt to identify the innovations required to overcome these limitations and todevelop new high-value applications that would make a scientific and social impact in the future.
Cijun Shuai, Wenjing Yang, Youwen Yang, Chengde Gao, Chongxian He, Hao Pan
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.207

Abstract:Mg alloys degrade rather rapidly in a physiological environment, although they have good biocompatibility andfavorable mechanical properties. In this study, Ti was introduced into AZ61 alloy fabricated by selective laser melting,aiming to improve the corrosion resistance. Results indicated that Ti promoted the formation of Al-enriched eutectic α phaseand reduced the formation of β-Mg17Al12 phase. With Ti content reaching to 0.5 wt.%, the Al-enriched eutectic α phaseconstructed a continuous net-like structure along the grain boundaries, which could act as a barrier to prevent the Mg matrixfrom corrosion progression. On the other hand, the Al-enriched eutectic α phase was less cathodic than β-Mg17Al12 phase inAZ61, thus alleviating the corrosion progress due to the decreased potential difference. As a consequence, the degradationrate dramatically decreased from 0.74 to 0.24 mg·cm-2·d-1. Meanwhile, the compressive strength and microhardness wereincreased by 59.4% and 15.6%, respectively. Moreover, the Ti-contained AZ61 alloy exhibited improved cytocompatibility.It was suggested that Ti-contained AZ61 alloy was a promising material for bone implants application.
Fan Feng, Jiankang He, Jiaxin Li, Mao Mao, Dichen Li
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.202

Abstract:Multimaterial bioprinting provides a promising strategy to recapitulate complex heterogeneous architectures of native tissues in artificial tissue analogs in a controlled manner. However, most of the existing multimaterial bioprinting techniques relying on multiple printing nozzles and complicate control program make it difficult to flexibly change the material composition during the printing process. Here, we developed a multicomponent bioprinting strategy to produce heterogeneous constructs using a microfluidic printhead with multiple inlets and one outlet. The composition of the printed filaments can be flexibly changed by adjusting volumetric flow rate ratio. Heterogeneous hydrogel constructs were successfully printed to have predefined spatial gradients of inks or microparticles. A rotary microfluidic printhead was used to maintain the heterogeneous morphology of the printed filaments as the printing path direction changed. Multicellular concentric ring constructs with two kinds of cell types distribution in the printed filaments were fabricated by utilizing coaxial microfluidic printhead and rotary collecting substrate, which significantly improves the printing efficiency for multicomponent concentric structures. The presented approach is simple and promising to potentially print multicomponent heterogeneous constructs for the fabrication of artificial multicellular tissues.
Rui Yao, Ahmed Yousef F Alkhawtani, Ruoyu Chen, Jie Luan, Mingen Xu
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.194

Abstract:Rapid reconstruction of functional microvasculature is the urgent challenge of regenerative medicine and ischemia therapy development. The purpose of this study was to assess whether hydrogel based microspheres coated by human umbilical vein endothelial cells (HUVECs) can direct rapid and efficient in vivo angiogenesis without the addition of exogenous growth factors or other supporting cells. Uniform alginate microspheres with adjustable diameter were biofabricated by electro-assisted bioprinting technology. Collagen fibrils were evenly coated on the surface of alginate microspheres via simple self-assembly procedure, and collagen concentration is optimized to achieve highest HUVECs adhesion and proliferation. Immunofluorescence staining and gene analysis confirmed the formation of prevascularized tubular structure and significantly enhanced endothelial gene expression. HUVECs-coated hydrogel microspheres with different diameters were subcutaneously injected in immune-deficient mice, which demonstrated rapid blood vessel regeneration and functional anastomosis with host blood vessels within one week. Besides, microsphere diameter demonstrated influence on blood vessel density with statistical differences, but showed no obvious influence on the area occupied by blood vessels. This study provided a powerful tool for rapid and minimal-invasion angiogenesis of bioprinting constructs and a potential method for vascularized tissue regeneration and ischemia treatment with clinically relevant dimensions.
Yi-Hsuan Ou, Yi-Hui Ou, Jing Gu, Lifeng Kang
International Journal of Bioprinting, Volume 5; doi:10.18063/ijb.v5i2.203

Abstract:Topical anesthetics are widely used in dental procedures. However, most commercially available medications are in the form of liquid or semisolid, which cannot provide prolonged effect intraorally. To address this issue, we proposed the use of three-dimensional printing (3DP) to fabricate a customizable dental anesthetic patch loaded with lidocaine that can be fitted perfectly onto the affected tooth. It has been shown that that patch can adhere on the tooth for more than 1 h, while releasing lidocaine from the patch made of hydrogels. In addition, the results illustrated the possibility of controlling the drug release profile by altering the shape of the patch, as well the use of a 3DP tooth model as the drug testing platform. Taken together, these data further reinforce the vast potential of the application of 3DP technology in personalized medicine.