Biophysical Journal

Journal Information
ISSN / EISSN : 00063495 / 15420086
Current Publisher: Elsevier BV (10.1016)
Former Publisher: Elsevier BV (10.1529)
Total articles ≅ 65,542
Google Scholar h5-index: 69
Current Coverage
SCOPUS
PUBMED
MEDLINE
MEDICUS
PMC
SCIE
LOCKSS
Archived in
SHERPA/ROMEO
Filter:

Latest articles in this journal

Michael J. Saxton
Published: 3 April 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.015

The publisher has not yet granted permission to display this abstract.
Hans Zoellner, Navid Paknejad, James Cornwell, Belal Chami, Yevgeniy Romin, Vitaly Boyko, Sho Fujisawa, Elizabeth Kelly, Garry W. Lynch, Glynn Rogers, et al.
Published: 31 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.013

Shintaroh Kubo, Tomohiro Shima, Shoji Takada
Published: 29 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.012

The publisher has not yet granted permission to display this abstract.
Besian I. Sejdiu, D. Peter Tieleman
Published: 21 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.008

Alexander Rohrbach, Tim Meyer, Ernst H.K. Stelzer, Holger Kress
Published: 14 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.005

The publisher has not yet granted permission to display this abstract.
Michael Bugiel, Mayank Chugh, Tobias Jörg Jachowski, Erik Schäffer, Anita Jannasch
Published: 14 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.02.030

The publisher has not yet granted permission to display this abstract.
Rebecca F. Alford, Patrick J. Fleming, Karen G. Fleming, Jeffrey J. Gray
Published: 14 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.03.006

The publisher has not yet granted permission to display this abstract.
Maximilian A.H. Jakobs, Kristian Franze, Assaf Zemel
Published: 14 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.02.031

The publisher has not yet granted permission to display this abstract.
Bibiana Onoa, Shingo Fukuda, Masakazu Iwai, Carlos Bustamante, Krishna K. Niyogi
Published: 13 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.02.029

Abstract:
Thylakoid membranes in chloroplasts contain photosynthetic protein complexes that convert light energy into chemical energy. Photosynthetic protein complexes are considered to undergo structural reorganization to maintain the efficiency of photochemical reactions. A detailed description of the mobility of photosynthetic complexes in real time is necessary to understand how macromolecular organization of the membrane is altered by environmental fluctuations. Here, we used high-speed atomic force microscopy to visualize and characterize the in situ mobility of individual protein complexes in grana thylakoid membranes isolated from Spinacia oleracea. Our observations reveal that these membranes can harbor complexes with at least two distinctive classes of mobility. A large fraction of grana membranes contained proteins with quasistatic mobility exhibiting molecular displacements smaller than 10 nm2. In the remaining fraction, the protein mobility is variable with molecular displacements of up to 100 nm2. This visualization at high spatiotemporal resolution enabled us to estimate an average diffusion coefficient of ∼1 nm2 s-1. Interestingly, both confined and Brownian diffusion models could describe the protein mobility of the second group of membranes. We also provide the first direct evidence, to our knowledge, of rotational diffusion of photosynthetic complexes. The rotational diffusion of photosynthetic complexes could be an adaptive response to the high protein density in the membrane to guarantee the efficiency of electron transfer reactions. This characterization of the mobility of individual photosynthetic complexes in grana membranes establishes a foundation that could be adapted to study the dynamics of the complexes inside intact and photosynthetically functional thylakoid membranes to be able to understand its structural responses to diverse environmental fluctuations.
Noah R. Cohen, Can Kayatekin, Jill A. Zitzewitz, Osman Bilsel, C.R. Matthews
Published: 12 March 2020
Biophysical Journal; doi:10.1016/j.bpj.2020.02.028

The publisher has not yet granted permission to display this abstract.